We narrowed to 790 results for: Cre
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TypeBlog PostPublishedMarch 2, 2021, 2:15 p.m.... colonies from one another in the challenge. To create a successful streak plate, first the bacteria are...
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New Viral Vectors - Spring 2025
TypeBlog PostPublishedApril 29, 2025, 1:30 p.m....Biosensor GENIE Project New serotype pENN.AAV.CMVs.Pl.Cre.rBG AAV5 Recombinase James M. Wilson New serotype... -
Addgene’s Blog Gets a New Look!
TypeBlog PostPublishedJan. 25, 2021, 5:54 p.m.... Just click on the search bar at the top of the screen and type in what you’re looking for. After you ... -
Grad School Advice Part 1: Picking a Lab and a Project
TypeBlog PostPublishedSept. 1, 2016, 2:30 p.m....friendly and full of useful advice. Stay tuned for concrete advice on how to pick a lab and a project. Listen... -
Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs
TypeBlog PostPublishedJune 15, 2016, 1:30 p.m....program. We are excited to discover what new and creative ideas applicants will propose for plasmids from... -
Addgene’s Top 10 Blog Posts from 2014
TypeBlog PostPublishedDec. 16, 2014, 8:01 p.m....scientists making their own cells to save money and create the tools that work best for their needs. If you... -
Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering
TypeBlog PostPublishedNov. 12, 2014, 3:54 p.m....CRISPR Libraries Enable Genome-Scale, Knockout Screening ... -
Synthetic Biology, Artificial Chromosomes, iGEM & More - Interview with Tom Ellis
TypeBlog PostPublishedNov. 29, 2017, 2 p.m.... of Cambridge before spending time in biotech screening direct interactions between drugs and promoters... -
What's New in CRISPR - Winter 2018
TypeBlog PostPublishedDec. 18, 2018, 1:17 p.m....variants of Cas nucleases. In doing so, they also created a modular toolkit for Cas-mediated genome engineering... -
500,000 Plasmids Shipped and Counting!
TypeBlog PostPublishedMarch 26, 2015, 3:24 p.m.... bacteria in stab culture format. Our lab team creates stabs for each plasmid being shipped based on the... -
Fueled by Coffee at #SfN14
TypeBlog PostPublishedNov. 18, 2014, 7:15 p.m.... #3431 if you’re at #SfN14 and meet our Addgenie crew and sign-up for a free plasmid! Also check out the... -
Plasmid Preps: Different Purity, Different Quantities, Different Uses
TypeBlog PostPublishedJune 16, 2020, 1:25 p.m....washing steps to favor the removal of this agent to increase transfection success. In conclusion, it is important... -
Addgene's Website Update
TypeBlog PostPublishedApril 8, 2024, 2:15 p.m....Deposit Materials” bar will prompt you to log in or create an account. Figure 2: The new deposit materials... -
A Conference By Postdocs For Postdocs: Future of Research
TypeBlog PostPublishedSept. 2, 2014, 3:05 p.m....early career scientists taking an active part in creating their own opportunities for career development... -
6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results
TypeBlog PostPublishedJune 26, 2014, 2:23 p.m....readers! The chromatograms in this article were created with SnapGene. ... -
Keeping Up With CRISPR/Cas9
TypeBlog PostPublishedMarch 11, 2014, 5:55 p.m....of this has basically happened in a year. It’s incredible.” Looking for CRISPR Plasmids? CRISPR-Cas9 ... -
Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)
TypeBlog PostPublishedJuly 26, 2018, 12:46 p.m.... key advantage of KTRs is that they are easy to create and simple to multiplex. Problems with FRET kinase... -
Science Communication for Everyone
TypeBlog PostPublishedApril 16, 2019, 1:05 p.m....Sadangi, a postdoc at the University of Toronto, created The Addictive Brain to spread awareness about how... -
Addgene's Top Blog Posts from 2020
TypeBlog PostPublishedDec. 29, 2020, 2:15 p.m....Joanne Kamens followed up with a blog post with creative ways to continue career development virtually.... -
CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping
TypeBlog PostPublishedNov. 29, 2018, 1:53 p.m....diagnostic toolkit, called DETECTR, which has been created by the authors. This kit uses Cas12 and Cas13 to... -
Your Top Requested Plasmid in 2016!
TypeBlog PostPublishedJan. 11, 2017, 8:47 p.m....SpCas9n (nickase), which nicks DNA rather than creating double stand breaks and may better facilitate ... -
Why Do I Need an MTA Anyway?
TypeBlog PostPublishedMay 1, 2014, 8:23 p.m....research results, modifications, and inventions created by the Recipient Right to file patent applications... -
Reflections and Looking Ahead
TypeBlog PostPublishedFeb. 25, 2025, 2:15 p.m.... challenges. Thank you for being part of this incredible journey. Together, we're not just sharing research... -
Our Most Popular Blog Posts from 2019
TypeBlog PostPublishedDec. 31, 2019, 2:26 p.m....documented many new tools on the Addgene blog and have created and updated guides on various topics in biology... -
Editor's Choice, August 2016
TypeBlog PostPublishedSept. 2, 2016, 1:32 p.m.... bit of a Twitter storm surrounding NgAgo, its creator, Han Chunyu of Hebei University of Science and ... -
Scientist Networking: What is an Informational Interview?
TypeBlog PostPublishedJuly 1, 2014, 3:42 p.m.... Check out Joanne's Reddit AMA It should be no secret that one of the best things you can do during your... -
Six Spooky Science Stories and Halloween at Addgene
TypeBlog PostPublishedOct. 31, 2019, 1:06 p.m.... appearance there too) and shared fascinatingly creepy science stories. Here are some of our favorite ... -
"What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring
TypeBlog PostPublishedDec. 17, 2013, 2:47 p.m....courtesy of jorenl/Vecteezy.com Helps set concrete goals and helps you reach your goals Listens well... -
Tag Your Favorite Yeast Genes with Ease
TypeBlog PostPublishedNov. 19, 2013, 2:37 p.m....multiple cloning site in place of the epitope tag for creation of your own unique protein fusions. This collection... -
Working 9-5: an Addgene Co-op
TypeBlog PostPublishedJune 2, 2022, 2:15 p.m....and Geneious, I was able to determine if any discrepancies between what we sequenced in house and the submitted... -
Who Gives a Tweet? 9 Facts About Scientists on Twitter
TypeBlog PostPublishedJune 26, 2017, 1:31 p.m....facts. Note: The images used in this post were created using data from or modified from Ke et al. 2017... -
Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot
TypeBlog PostPublishedDec. 5, 2019, 1:49 p.m....Addgene. Addgene's new homepage While users are increasingly accessing websites other than through the ‘front... -
A Better Way to Get Customer Support: The Help Center
TypeBlog PostPublishedDec. 10, 2015, 3:30 p.m....support for our resources with our new Help Center! Created using the same reliable Zendesk system that powers... -
pSiM24: Simplifying Plant Genetic Engineering
TypeBlog PostPublishedSept. 29, 2015, 2:30 p.m....preparation in E. coli. Addgene depositor Indu Maiti has created a new and versatile binary Ti vector for both transient... -
6 Steps to Submitting a Resume That Gets Seen
TypeBlog PostPublishedJuly 9, 2015, 2:30 p.m....of the job application process can dramatically increase the chances of being seriously considered for ... -
In Living Color: The Skinny on In Vivo Imaging Tools
TypeBlog PostPublishedMarch 27, 2014, 5:14 p.m....PAiRFP2, to the in vivo imaging toolbox, which increase their fluorescence upon illumination with far-... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog PostPublishedDec. 3, 2020, 2:15 p.m....Figure 1. The RepCap plasmid and its products. Created with BioRender.com Replication (rep) gene... -
Plasmids 101: What is a plasmid?
TypeBlog PostPublishedApril 2, 2020, 6:17 p.m.... foreign DNA into another cell. Minimally, lab-created plasmids have an origin of replication, selection... -
Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process
TypeBlog PostPublishedFeb. 1, 2018, 3:07 p.m....restriction enzymes, but you will also be able to create custom annotations and design additional experiments... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog PostPublishedJuly 16, 2019, 12:59 p.m....spinal cord neurons using the Gal4/UAS system. This created fish lines with different groups of neurons that... -
Bringing Sustainable Practices to the Lab: Recycling
TypeBlog PostPublishedFeb. 14, 2019, 2:35 p.m....packaging Packaging is a huge contributor to the increase in plastic manufacturing, accounting for 40% of... -
Build Your CRISPR Vocabulary
TypeBlog PostPublishedJuly 2, 2024, 1:15 p.m....mutating these sequences. Pro tip! Various labs have created Cas9 enzymes with looser PAM requirements, which... -
Antibodies 101: Affinity Tags
TypeBlog PostPublishedSept. 19, 2023, 1 p.m....resin. The MBP tag, although large, can actually increase expression and solubility of the tagged protein... -
Antibodies 101: Fc Effector Functions
TypeBlog PostPublishedMay 18, 2023, 1:15 p.m....immune response against a pathogen. The Fc domain creates a link between the antibody and other parts of ... -
CASTing Off for New Shores in Human Genome Editing
TypeBlog PostPublishedJune 10, 2025, 1:15 p.m....genomic DNA downstream of gRNA-specified sites. Created with BioRender.com. CASTs quickly became popular... -
Oh, The Places You Can Go: Careers in Science Communication - Science Writing
TypeBlog PostPublishedOct. 3, 2017, 12:11 p.m.... at eScience Labs, an educational company that creates hands-on science lab kits and digital curriculum... -
FastCloning: A Newer, Simpler Cloning Technique
TypeBlog PostPublishedNov. 1, 2016, 2:30 p.m.... the correct size (vector + insert). You should screen 3-4 colonies to ensure that you find the correct... -
Management for Scientists: Giving Feedback
TypeBlog PostPublishedFeb. 6, 2015, 2:46 p.m....find this hard so get used to it by making it a concrete goal to say “thank you, job well done” to one ... -
Advice for Choosing a Research Project
TypeBlog PostPublishedApril 17, 2014, 2:45 p.m.... of combining “new discoveries or technologies increase chances that your project will be fresh as well... -
The Stingy Scientist: How the Baby Gel Box Was Born
TypeBlog PostPublishedApril 15, 2014, 3:11 p.m.... paying attention to living a “green” life and creating less waste. Most communities seem to have recycling...