We narrowed to 812 results for: Cre

...antibody” can be used for downstream IP/ChIP assays. Creating epitope-tagged clonal cell lines remains a challenge...crRNA complementary to the template strand could increase knock-in efficiency.Note: Our TAG-IN tool can ...

..., while REC includes most of the bridge helix. Created with BioRender.com.   Cas9 has two major lobes...guide RNA, dark blue; DNA, gray; PAM site, yellow. Created with BioRender.com.   The exact conformational...

...process. To deposit plasmids online, you will need to create an account with Addgene before starting the process...plasmids, and adding all the plasmids at once increases the likelihood that the plasmids are given sequential...

... cancer and your continued work is necessary to create therapeutics to cure the cancer. Authorship:  Publishing...translations of documents that are not in English. Create a Google Scholar account Sanitize your LinkedIn...

...impressive entries, but we have faith our community of creative scientists will deliver some fabulous photos again...

...added new sections to share and describe some incredible new CRISPR technologies developed and widely ...

...gave to me: CRISPR activation and interference screens for primary human T cells🎵 On the 3rd day of CRISPR...

...can't celebrate Addgene without recognizing our incredible depositors and requestors. Thank you all so much...

...came out last year on sucrose supplementation to increase AAV2 yields and we’re eager to share more! Lastly...

...interrogate many scientific questions. But these tiny creatures do tend to come with a rather astonishing ability...

... showed that a laughter program significantly decreased immunological markers of stress in adolescents...

...Addgene Videos Page 5 Science Rap Videos and Their Creator: Tom McFadden Using Video to Share Your Science...

..., Korea, Japan, India, and Brazil.  We've also created a robust support system for scientists using materials...

...answers! via GIPHY   We’ve been working hard to create the most informative and entertaining protocol ...

... This plasmid has been used in studies of the secretome, ubiquitin ligase, cellular senescence, autophagy...

...kb ladder. Using a commercial plasmid prep kit increases the costs to $20-30 per ladder: still significantly...

...’s lab. X-Chime and Performing In Vivo CRISPR Screens Using the FITS Approach Caitlin Faulds, who introduced...

...early July the Town of Watertown announced the creation of an “electricity aggregation program” for all...

... colonies from one another in the challenge. To create a successful streak plate, first the bacteria are...

...a plethora of options, ranging from Affie to Yncredibody. After an agonizing week spent in cloistered ...

... Just click on the search bar at the top of the screen and type in what you’re looking for. After you ...

...Biosensor GENIE Project New serotype pENN.AAV.CMVs.Pl.Cre.rBG AAV5 Recombinase James M. Wilson New serotype...

...friendly and full of useful advice. Stay tuned for concrete advice on how to pick a lab and a project. Listen...

...program. We are excited to discover what new and creative ideas applicants will propose for plasmids from...

...scientists making their own cells to save money and create the tools that work best for their needs. If you...

...CRISPR Libraries Enable Genome-Scale, Knockout Screening ...

...variants of Cas nucleases. In doing so, they also created a modular toolkit for Cas-mediated genome engineering...

... of Cambridge before spending time in biotech screening direct interactions between drugs and promoters...

... bacteria in stab culture format. Our lab team creates stabs for each plasmid being shipped based on the...

... #3431 if you’re at #SfN14 and meet our Addgenie crew and sign-up for a free plasmid! Also check out the...

...washing steps to favor the removal of this agent to increase transfection success.  In conclusion, it is important...

...Deposit Materials” bar will prompt you to log in or create an account.  Figure 2: The new deposit materials...

...early career scientists taking an active part in creating their own opportunities for career development...

...readers! The chromatograms in this article were created with SnapGene.    ...

...of this has basically happened in a year. It’s incredible.” Looking for CRISPR Plasmids? CRISPR-Cas9 ...

... key advantage of KTRs is that they are easy to create and simple to multiplex. Problems with FRET kinase...

...Sadangi, a postdoc at the University of Toronto, created The Addictive Brain to spread awareness about how...

...SpCas9n (nickase), which nicks DNA rather than creating double stand breaks and may better facilitate ...

...Joanne Kamens followed up with a blog post with creative ways to continue career development virtually....

...diagnostic toolkit, called DETECTR, which has been created by the authors. This kit uses Cas12 and Cas13 to...

...research results, modifications, and inventions created by the Recipient Right to file patent applications...

...documented many new tools on the Addgene blog and have created and updated guides on various topics in biology...

... challenges. Thank you for being part of this incredible journey. Together, we're not just sharing research...

... bit of a Twitter storm surrounding NgAgo, its creator, Han Chunyu of Hebei University of Science and ...

...  Check out Joanne's Reddit AMA It should be no secret that one of the best things you can do during your...

... appearance there too) and shared fascinatingly creepy science stories. Here are some of our favorite ...

...courtesy of jorenl/Vecteezy.com Helps set concrete goals and helps you reach your goals Listens well...

...multiple cloning site in place of the epitope tag for creation of your own unique protein fusions. This collection...

...Addgene.  Addgene's new homepage While users are increasingly accessing websites other than through the ‘front...

...facts. Note: The images used in this post were created using data from or modified from Ke et al. 2017...