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...1414 (2018). 10.1101/gr.237362.118 Chen, G. Y., Hur, S. Cellular origins of dsRNA, their recognition and ...

...reporter podcast segment Discovered in the early 1990’s, microRNAs (miRNAs) are recognized as important regulators...

...single expression cassette is advantageous to Cas9’s larger expression requirements (Figure 1), but Cas12a...

... to do once you’ve chosen a gRNA and modification(s) Congratulations! You’ve picked your perfect guide...

... consistently engage with others about your topic(s) of interest. Hopefully through these links, I’ve:...

...This Publication at Addgene 4. Petroni, G., Spring, S., Schleifer, K.-H., Verni, F. & Rosati, G. Defensive...

...Xie K, Minkenberg B, Yang Y. Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5. doi: 10.1073/pnas.1420294112...

...input. Usually that function is in the shape of an S; there is a narrow threshold like a switch that turns...

....1177/34.6.3084626  Zhang Q, Miyamoto A, Watanabe S, Arimori T, Sakai M, Tomisaki M, Kiuchi T, Takagi ...

...active or “on.” Dronpa’s color thus indicates MEK1’s current state (Green when off, dark when on). Figure...

...lentiviral backbone from the Zhang lab expressing S. pyogenes Cas9 and a gRNA cassette. It can be used...

... guide AAV Viral Preps References  Chen, Y., Hu, S., Lee, W., Walsh, N., Iozza, K., Huang, N., Preston...

...By Brook Pyhtila One drawback to the commonly used S. pyogenes CRISPR/Cas9 system is that it is too large...

...References Boguszewska, K., Szewczuk, M., Urbaniak, S. et al. Review: immunoassays in DNA damage and instability...

...Together these two experiments determined that ChRger2’s improved light sensitivity and higher-photocurrent...

...audience is expending to pay attention to the mode(s) of communication you choose.  Audience knowledge ...

...microenvironments have started to gather attention. By the 1960’s, scientists have begun to understand two main areas...

...Many thanks to our guest blogger Gary McDowell! Gary S. McDowell is currently the Executive Director of Future...

...Pubmed Central PMCID: PMC4000813. 2. McDowell, Gary S., et al. "Shaping the Future of Research: a perspective...

...that ultimately builds up to an overall conclusion(s). In some articles, the data are presented to build...