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Showing: 261 - 280 of 507 results

  1. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...different bacterial hosts (Doran et al. 1998). Regulatory elements within the ori also control copy number...own replication machinery such as Rep or other initiator proteins, they do not require host proteins for...

  2. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...versatility and standardization of genetic tools in the laboratory mouse, most optogenetics is performed in this....    References 1. Stuber, Garret D., et al. "Excitatory transmission from the amygdala to nucleus accumbens...

  4. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...It helps to come from the lab of a well-known investigator. If you only have middle author papers or no...Einstein College of Medicine in the Department of Anatomy and Structural Biology, and now serves as the Director...

  5. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...delivered through transfection of naked DNA. Other investigators who had been working to develop high efficiency...line and ~8 and 9% efficiencies in T-cells and hematopoietic stem cells respectively. Future researchers ...

  6. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...phosphorylation sites. If knowledge of a candidate regulatory kinase is known, it’s possible to generate non-phosphorylated...Often, however, researchers lack knowledge of the regulatory kinase, access to active kinase for the reaction...

  7. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...turned to the CRISPR/Cas9 Synergistic Activation Mediators (SAM) system available from Addgene. This system...W. CRISPR/gRNA-directed synergistic activation mediator (SAM) induces specific, persistent and robust ...

  8. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...know is your area code. Senators: To find contact information for your senators all you need to do is select...

  9. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    Published
    Nov. 25, 2015, 4 p.m.
    ...academic publishing goals and make you a better communicator overall. Don’t Write in a Vacuum Your research...through many iterations read by your PI, your collaborators, others you know in the field, and even people...

  10. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...history, principles, and types of ELISA, and our laboratory experience with peptide/protein analyses using...10.1016/0003-2697(81)90281-5 Pillai-Kastoori L, Heaton S, Shiflett SD, Roberts AC, Solache A, Schutz-Geschwender...

  11. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...nicking/cleaving of DNA occurs, not for deadCas activator/repressor experiments where only localization ...RNA-seq would be an appropriate method for an activator or repressor Cas. Similarly, Cas ChIP-Seq would...

  12. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...DNA transposons are most commonly used in the laboratory for genome manipulation. When transposons are...2020) Jump around: transposons in and out of the laboratory. F1000Res 9:135. https://doi.org/10.12688/f1000research...

  13. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...postdoc” is, and that “PI” stood for principal investigator. In basic science (as opposed to translational...in a lab. Position Common tasks Principle investigator (PI) Runs the lab Writes a lot of grants Varying...

  14. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...reliably detect single-base differences. The original creators of this technique visualized the ligation product... the Thermostable Ligase Reaction Temperature Calculator can be used to estimate an appropriate incubation...

  15. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...first one and can be used routinely in a research laboratory. The risk of generating RCL is low but extra ...such as Doxycycline (Dox), the Tet-controlled transactivator (tTA, also expressed by your transfer vector...

  17. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...environment? If these are pressing issues in your laboratory, then FRET experiments are a viable biophysical...and purification is a snap using thin layer chromatography (Fessenden, 2009). However, these reagents ...

  18. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ... unbiased discovery. With these screens, the investigator generates a cell population where all genes ...contexts. Most of the immune cells we study are migratory, and their exact localization is important for...

  19. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...absorbed by matter, they promote electrons within atoms to a higher energy state. This process is called.... His projects include investigating negative regulators of T cells and cytokine signaling in tumor immunity...

  20. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ... electrophysiology data too but that’s what collaborators are for). For example, if you study neural pathways...projection-specific neuronal populations using combinatorial viral approaches. Current protocols in neuroscience...
Showing: 261 - 280 of 507 results