We narrowed to 662 results for: Clu

...Acoustic Reporter Genes (ARGs) are engineered gene clusters that encode proteins that form bacterial gas vesicles...have hollow interiors and a protein shell that excludes water but is gas permeable. These vesicles are...

...biosensors as an example. Many calcium biosensors include GFP fused to calmodulin (CaM) and the chicken myosin... (ER), vacuole or cellular membranes. Examples include blue flame mitochondria-targeted calcium sensor...

...members of the ubiquitous activin ligand family, including Activin A. A commercially developed therapeutic...analytical development. His areas of expertise include early stage drug discovery and pre-clinical development...

...appreciated and valued This often stems from being included in organizational decision-making. It is imperative...things, diversity of responsibilities, which might include training or teaching others, research, and policy...

...technological biological blockbuster known as the CRISPR (clustered regularly interspaced palindromic repeats) Cas...in E. coli in 1987 (1), the concept that these clustered repeat sequences operated as a safeguard against...

...control Historically, AAV vector QC has been sparse including only titration of AAV particles and a protein ... the expression cassette that we want, it also includes a small amount of spurious DNA from the packaging...

...and is based on the pTRC99a plasmid. It does not include the PBAD promoter. If you already have a pBAD33...the plasmids deposited from Di Ventura's lab, including the popular light-inducible nuclear export system...

...Would you buy and use a plasmid if it didn’t include any plasmid sequence data? Of course not. Unfortunately... plasmid-based and offer numerous advantages, including more secure archiving, unambiguous molecular definition...

...but suffer from several important shortcomings including low processivity (ie. the probability of the polymerase...transport from the cell surface. Their toolkit includes a set of plasmids each containing an anti-GFP ...

...found in bacteria. Some common ones you might see include ColE1, pMB1 (which comes in a few slightly different...questions to get started: Will the plasmid be used exclusively in E. coli? Gram negative bacteria in general...

...association, started a speaker series or biotech club, managed their own blog or podcast, initiated and...future and the 1:1 format is good for everyone including those that find events stressful. Video chatting...

... to do the research itself. Several years of exclusively writing lab reports and empirical essays had ...of those entering science communication (myself included) come from a scientific background and bring with...

...module. As a general guideline, experiments that include growing plants require pots, trays, soil, fertilizer...efforts. Our education partnerships have grown to include local school districts, professional education ...

...standardized methods for evaluating CRISPR technologies, including the ever important on/off-target efficiency. Likewise...guest bloggers Dominik Paquet and Dylan Kwart, including a detailed look at how cut-to-mutation distance...

...number of unique features compared to Cas9. These include: More targeting flexibility Unlike Cas9 and the...base excision would alter the target sequence and preclude future HDR, which could lower HDR efficiency. ...

...napkin for paper, a small group of scientists including Stanley Falkow, Stanley Cohen, Herbert Boyer, ...eBook. Some highlights of the 2nd edition eBook include: new chapters on genome engineering, cloning, and...

...month.  We are currently focused on two projects, including one led by a talented biomedical engineering team...still able to get through to a number of experts, including Harvard/MIT faculty, venture capital and angel...

...the specificity of their luciferase assay, they concluded that components in the buffer used for protein...works in multiple types of primary mouse cells, including neuronal and gut stem cells, dendritic cells, ...

...multidirectional control, a new study repurposes the sgRNA to include modular synthetic RNA scaffolds that can recruit...RNA in the CRISPRi system can be repurposed to include modular RNA scaffolds. These RNA scaffolds can...

...creative thinking Enhance leadership skills Support inclusion, diversity, and open communication Feel valued...download the "Mentoring for Scientists" ebook, which includes the entire series of mentoring blog posts, the...