We narrowed to 364 results for: DUR
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TypeBlog PostPublishedMarch 24, 2020, 1:15 p.m....further streamline this process and/or stabilize RNA during tissue collection and homogenization. The most ...
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Visualizing Protein Turnover In Situ
TypeBlog PostPublishedJan. 16, 2018, 3:20 p.m....influence behavior. A conference and a breakthrough! During my graduate studies I attended a scientific symposium... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog PostPublishedMarch 19, 2019, 1:31 p.m....benthamiana or Arabidopsis thaliana (Xing et al., 2016)). During Agrobacterium-mediated DNA transfer, each GOI is... -
Reproducibility for Everyone: Lessons from an Open Science Collaboration
TypeBlog PostPublishedFeb. 28, 2019, 1:34 p.m.... everything that you would want to know before, during, and after organizing such a workshop. Finally,... -
RaPID Detection of RNA-protein Interactions
TypeBlog PostPublishedAug. 16, 2018, 12:40 p.m....events that occur on a short timescale, such as during drug action or cell signaling. Limitations of RaPID... -
dTAG - You're it!
TypeBlog PostPublishedJune 21, 2018, 2:06 p.m....additionally provides detailed information on cloning procedures used to construct the deposited plasmids. Molecules... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog PostPublishedJune 15, 2017, 2:30 p.m.... and the concentration of the protein solution. During the experiment, the solution is diluted, complicating... -
Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog PostPublishedJune 8, 2017, 2:30 p.m....or little deterioration or change of the signal during the course of the experiment. The best fluorescent... -
Plasmid Tools for Microbiome Studies
TypeBlog PostPublishedJune 27, 2019, 12:25 p.m....one bright particle that segregates into one cell during cell division. To achieve the agglomeration of ... -
Summer SciComm: Preprints
TypeBlog PostPublishedAug. 16, 2022, 1:15 p.m....He also posted two preprints of his own research during his PhD studies. References and Additional Resources... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog PostPublishedJuly 21, 2016, 2:30 p.m....sex, and it’s important to analyze these factors during an experiment. In the case of stem cells, these... -
Plasmids 101: Cre-lox
TypeBlog PostPublishedJan. 13, 2015, 3:47 p.m....are active only in certain cell or tissue types, during certain stages of development, or by making the... -
5 Steps to Writing Grant Proposals for Grad Students
TypeBlog PostPublishedFeb. 11, 2014, 12:13 p.m.... your work, your advisor may want to mentor you during the grant application process. When my PhD advisor... -
Design Tips for Prime Editing
TypeBlog PostPublishedJan. 23, 2025, 2:15 p.m....the cell to use the edited strand as a template during DNA repair. Test multiple nick sites, starting ... -
Viral Vectors 101: Chemogenetics
TypeBlog PostPublishedJuly 11, 2023, 1:15 p.m....Carpenter JC, Snowball A, Knauss S, von Schimmelmann M, During MJ, Lignani G, Schorge S, Young D, Kullmann DM,... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog PostPublishedDec. 14, 2021, 2:15 p.m....that site. So, when DNMT1 methylates the genome during DNA replication, it doesn’t methylate sites blocked... -
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog PostPublishedAug. 24, 2021, 1:15 p.m....the protein-antigen complexes will be washed away during the protocol. The amount of antibody that binds... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog PostPublishedJune 25, 2020, 1:15 p.m....it solidified some concepts that are heard often during undergrad. Lauren Flynn is the Addgenie behind... -
Split-BioID: An Improved Method for Studying Protein-Protein Interactions
TypeBlog PostPublishedMay 1, 2018, 1:57 p.m....interactions to remain stable (or to be stabilized) during cell lysis and lysate fractionation. Important ... -
Lab Automation at Addgene
TypeBlog PostPublishedMarch 22, 2018, 1:28 p.m....pipette samples in and out of it, you can set up a procedure for it using the Biomek FxP. For example, we added...