We narrowed to 543 results for: EPO

... assay, the pseudoviruses express a luciferase reporter which is used as a readout for infection of ACE2...robust immune response. The Balazs lab was quick to deposit their plasmids at Addgene to accelerate ongoing...

...Last month, the David Root and John Doench labs deposited new and improved libraries called Julianna, for...knockout screens. The Jonathan Weissman lab has deposited genome-wide CRISPR activation (CRISPRa) libraries...

...primary antibody that is directly conjugated to your reporter circumvents the issue. However, directly conjugated...which uses just a primary antibody that has your reporter (in this case a fluorophore) directly attached...

...money producing virus from select vectors in the repository yourself, you can now order ready-to-use virus...of the most popular lentiviral vectors in the repository are used to produce CRISPR tools, we’re starting...

...While the vast majority of CRISPR plasmids in our repository use Cas9 to cut DNA, Addgene Science Communications...Resources Learn More about Viral Vectors Browse the Repository  ...

...calcium-binding sites as possible for added sensitivity. As reported in Nature Methods, the FRET changes of these Twitch...magnesium sensor, the first of its kind. “I hope by depositing at Addgene, people will start using them,” Merkx...

...career exploration site Inventing Heron has a radio reporter challenge, and code.org has coding tutorials for...experiments carefully. Consider submitting a registered report, where peer review is based on the methods, not...

... plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. Think we missed...ubiquitous promoter drives expression of an mCherry reporter.  After using mCherry for FACS sorting of the ...

...lentiviral preps of select lentiviral vectors from the repository for you. We perform test infections with all ...lentiviral format Another popular set of tools in the repository are CRISPR pooled gRNA libraries that can be ...

... fields, including synthetic biology. Addgene depositors Christopher Voigt and Jeffrey Tabor have been...phosphorylation, promoting transcription of a GFP reporter. Subsequent exposure to red light rapidly deactivates...

...antibodies expressed from plasmids that have been deposited with Addgene. Here, we take a closer look at the... VH and VL sequence. These plasmids have been deposited with Addgene for distribution to the scientific...

...s take a look at a few examples with plasmids deposited at Addgene.  PRESTO-TANGO: The ligands that activate...which can then activate expression of a luciferase reporter in the nucleus. The Roth lab showed that this ...

...off for the day. 1:45 PM--Write final report. Work on a final report for a user research project I led. ...

...activated Ras/Erk signaling by using a fluorescent reporter for Erk kinase translocation. These results demonstrate...control protein activity Read about RANbodies, reporter nanobody fusions Learn more about using the secondary...

...populations or a huge smear across your FSH vs reporter plot. In this post, I’ll walk you through how ...Purification Protocol Video rAbs in the Addgene repository...

...ability to infect cells with a high efficiency and deposit a DNA/RNA cargo into their targets makes them attractive...Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors ...

...To make luminescent imaging a reality, Addgene depositor Takeharu Nagai and colleagues at Osaka University...binding. Suzuki et al. also envision eNLs as good reporter genes for endogenous protein fusions created using...

... information on each cancer clinical sequencing report includes rare variants that haven’t been characterized...MAPK1/ERK2 Missense Mutants. Brenan L et al. Cell Reports, 2016. PubMed PMID: 27760319. PubMed Central PMCID...

... get started with mentoring. Read this Catalyst Report “Making Mentoring Work” to learn about what makes...make your mentor want to keep working with you is report back with progress. Tell them how their advice ...

...Ramanathan et al. also used the Contamination Repository for Affinity Purification (CRAPome), to identify...identify highly biotinylated background proteins reported by other research groups that performed BirA*-based...