We narrowed to 285 results for: Fus
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TypeBlog PostPublishedDec. 14, 2017, 2:08 p.m.... Our Stem Cell Collection Transcription Factor Fusions for Enhanced Reprogramming Find plasmids from the...
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Hot Plasmids Spring 2024
TypeBlog PostPublishedMay 23, 2024, 1:15 p.m....Building on their earlier Voltron indicators (a fusion of Ace2N and HaloTag, Figure 4), they determined... -
An Inside Look into Addgene's Dev Team
TypeBlog PostPublishedJan. 23, 2020, 2:45 p.m....might be calling it an aliquot. This can lead to confusion as to what the code is supposed to be doing. When... -
CRISPR Activation: A Practical Guide
TypeBlog PostPublishedAug. 18, 2016, 2:30 p.m....for the upregulation of essentially any gene by fusing mutants of Cas9 deficient in DNA cutting activity... -
Tips for CRISPR Gene Editing in Mice
TypeBlog PostPublishedJune 28, 2016, 10:59 a.m....the microinjection was done as the pronuclei were fusing, you may have several mosaic mice. To figure out... -
Transferable Skills Guide: Conflict Resolution
TypeBlog PostPublishedJan. 4, 2022, 2:15 p.m....taking care of that”, because that can indicate confusion around... Ownership: Misunderstandings about who... -
Quick Guide to Near-Infrared Fluorescent Proteins
TypeBlog PostPublishedOct. 5, 2021, 1:15 p.m....less interference with the oligomeric state of a fusion partner. Therefore, we recommend using miRFPs and... -
Five Popular Model Organisms, Part 2
TypeBlog PostPublishedSept. 29, 2020, 1:15 p.m....tool-kit from the Hamdoun Lab to create fluorescent fusions in a multitude of organisms including Xenopus. ... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog PostPublishedJan. 29, 2019, 2:27 p.m.... composed of a “Top” and “Bottom” portion, each fused to a separate piece of RNA. Fluorescence is observed... -
Antibodies 101: Beyond Surface Labeling
TypeBlog PostPublishedJune 27, 2024, 1:15 p.m....visualizing DNA instead of proteins. Some stains can diffuse through the cell membrane freely, while others ... -
Tips for Getting a Faculty Position
TypeBlog PostPublishedJuly 20, 2023, 1:15 p.m....answer an expert's question than to leave everyone confused about your research because you waded too deep... -
Adeno-associated Viruses (AAVs) for Genome Editing
TypeBlog PostPublishedMarch 27, 2018, 1:32 p.m....templates for applications that require large gene fusions. The current state-of-the-art in combining the ... -
An Integrin Antibody Toolkit from IPI
TypeBlog PostPublishedMarch 28, 2024, 1:15 p.m....antibodies composed of rat or mouse variable regions fused with rabbit constant domains. All bind specific ... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog PostPublishedMay 10, 2016, 2:30 p.m....available from Addgene. This system uses the dCas9-VP64 fusion commonly used to activate transcription, but with... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog PostPublishedApril 24, 2015, 2:39 p.m....techniques, such as Golgi staining, injection of diffusible labels, or electron microscopy of stained sections... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog PostPublishedMay 23, 2023, 1:15 p.m.... antibody against your target protein, consider fusing a protein tag to the target and using an epitope-based... -
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility
TypeBlog PostPublishedMay 18, 2018, 7:32 p.m....actually performed, potentially curtailing future confusion (and hours sifting through paper lab notebooks... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog PostPublishedJuly 12, 2016, 2:30 p.m.... of the skyline of Acadia National Park. I was confused. It was supposed to be hard. I was mentally prepared... -
Important Considerations When Using AAVs
TypeBlog PostPublishedJune 13, 2017, 2:30 p.m....axonal transport desirable or not desirable? If diffuse gene expression is tolerated, then axonal transport... -
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
TypeBlog PostPublishedMarch 7, 2017, 3:30 p.m....minimize off-target effects of CRISPR-Cas9. Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF...