We narrowed to 943 results for: Gene

... popular tools for genetic screens, enabling genome-wide interrogation of the genes required for cellular...that constitutively express Cas9 and screen for genes that affect Th2 differentiation and activation, ...inserting the NanoLuc luciferase downstream of the apoB gene. Using this reporter, the researchers explore many...-LPs and accelerate the discovery of additional genes and small-molecule probes to aid in the understanding...transduction, now enabling forward genetic screens in cells with few genetic tools. Find the mouse CRISPR ...plasmids at Addgene. Liu et al., Nature Communications, 2019. PubMed PMID: 31451697. Genetically encoded ...responsible for a phenotype can be identified by next-generation sequencing of the genomic DNA. However, some ...

...ampicillin resistance gene, you’ll find the full name of the gene, the name of its gene product, and a description... plasmid features such as antibiotic resistance genes, selectable markers, promoters, origins, tags, and... Overview of SnapGene powered plasmid maps at Addgene  Three factors make our SnapGene powered plasmid...powered by GSL Biotech's SnapGene Server Software. With the backing of SnapGene’s sequence viewer software...the features you need quickly. SnapGene’s Feature Library - SnapGene’s software seamlessly identifies ...scientists) for suggesting these changes, and the SnapGene and Addgene software development teams for working tirelessly...example below. Our old map is on the left while the SnapGene powered map is on the right (click here to see...

...for its powerful promoter of a gene important for methanol usage. This gene promoter, AOX1, was co-opted...experimental model. In fact, approximately 25% of yeast genes have a human counterpart, enabling study of human-relevant...Saccharomyces cerevisiae to human: The important gene co‑expression modules. Biomedical Reports 7.2 (2017...fast (doubling time of ~ 2 hours) Amenable to genetic manipulation The first eukaryote (S. cerevisiae... new at all, but it’s not as well-known in the general research community. Phillips Petroleum Company...release the K. phaffii expression system to the general research community in the 1990s when they abandoned...the strain tractable for peroxisomal studies on biogenesis, degradation, and proliferation. Genome manipulation...

... for gene expression, turning transcription on and off by binding proximal to their target genes. Since...Since gene expression patterns determine everything from stem cell differentiation fate to tumor suppression...from the library to further investigate specific genes and TF isoforms. Due to the size of the collection...use to generate the relevant disease model. Accurate modeling of a disease depends on both genetic and expression... not yet available, just ask an Addgene scientist at help@addgene.org! References and Resources  References...scientific community. Recently, the Zhang lab generated a TF library (aka the MORF Collection) and an ...isoforms transcribed from these sites. The Zhang lab generated a comprehensive library of each unique TF open...

....  Additional resources on the Addgene blog Subscribe to the Addgene blog Read our sustainability in...requires a significant energy expenditure here at Addgene. These are critical systems that cannot be removed...only sustainability effort we’ve implemented at Addgene. The kitchen and office areas provide multiple ... the lab as well. For a more in-depth look at Addgene’s current lab-oriented environmental efforts check... about conservation in the lab Resources on Addgene.org Sign up for our quarterly newsletter Help other...resources by sharing plasmids Find plasmids at Addgene ...

...social media. #AddgeneCRISPRweek on Twitter From May 13 (today!) to May 17, the Addgene Twitter feed will...CRISPR overview and history. Tuesday will focus on generating knockouts using CRISPR and Wednesday will highlight...CRISPR. Hope you will join us this week at #AddgeneCRISPRweek. We updated the CRISPR guide! If you’re not...

...reprogramming transgenes and the up-regulation of corresponding endogenous pluripotency genes. Retroviral...disrupt the expression of tumour suppressor genes and/or oncogenes, potentially leading to the presentation... Shao, L., & Wu, W. (2010). Gene-delivery systems for iPS cell generation. Expert opinion on biological...a single polycistronic vector or multiple single-gene vectors can be used to package reprogramming factors... the transposase. The donor plasmid contains the gene(s) of interest flanked by terminal repeat sequences...demethylation and expression of endogenous pluripotency genes (i.e. Oct4) are other measures of reprogramming....pluripotency: history, mechanisms, and applications." Genes & Development 24, no. 20 (2010): 2239-263. PubMed...

...Resources from the Addgene Blog Addgene's a nonprofit? Get an Inside Look at Addgene's NGS Quality Control... In this episode of the Addgene podcast, we sit down with Addgene’s Founders - Melina Fan, Ken Fan, and...interview with Addgene's founders Podcast breakdown Intro Chapter 1 - Who are Addgene's Founders? Chapter...Chapter 2 - The process of founding Addgene, where the idea for Addgene came from and how the idea got off...Chapter 6 - Developing Addgene’s Brand and culture Chapter 7 - Building Addgene as a global organization...current roles at Addgene and beyond - Ginkgo, Tufts Launchpad, and more Chapter 10 - Addgene's Future, the ...efforts at Addgene as the Chief Financial Officer and Chief Operating Officer. Prior to Addgene, Kenneth...

...utilizes grants to support our own initiatives, Addgene is deeply concerned about these developments. These... We Are Here to Help Despite these challenges, Addgene remains steadfast in our mission to accelerate ...vital during this period of constrained funding. Addgene is uniquely positioned to help research institutions...Universities and research institutions can turn to Addgene as a trusted partner for archiving their biological...scientific progress depends on our collective response. Addgene stands ready to collaborate with partners across...work and what support would be most valuable from Addgene during this time. Your input will directly shape...scientific community. Let us know by emailing help@addgene.org. We look forward to hearing from you!...

...allow people to add a gene to a repository others could request from — Addgene!  Addgene was established as...information across the globe.  “Read the Addgene guide: https://www.addgene.org/guides/crispr/” — SirHamhands, ... which continue to grow today. “I generally refer people to Addgene for the basics of molecular biology...celebrating Addgene's 10 year anniversary.   Expanding our work In March 2015, Addgene reached the...a resource like Addgene to all get the same virus.” — Grad student visiting Addgene’s booth at the Society...Addgene is ending 2024 with big plans for our future. And while we’ve been excited to share them with...thoughts from the community that makes us… us. “The Addgene not-for-profit is an amazing service to biology...

...perhaps are a little limited on time — check out Addgene’s new Quick Tip series on YouTube! In this series...

... page of the Addgene Data Hub.   Antibody data reports can be searched and sorted by gene/target, name... on addgene.org Addgene's Antibody Collection Addgene's AAV Data Hub More resources on the Addgene blog... request for your experiment!  Addgene’s Antibody Data Hub Addgene’s Antibody Data Hub can help with ...open-access Data Hub, researchers interested in the Addgene antibody collection can look at user-deposited ...experimental conditions in which the data was generated, you can scroll down the page to the materials...each data submission we receive is checked by Addgene's curation team for accuracy and completeness. The... citation. If you’re considering requesting an Addgene antibody, the Data Hub can help you decide if an...

...group of talented individuals that makes Addgene — well, Addgene. We're so grateful for each and every one...This year marks Addgene's 20th anniversary! We've been celebrating throughout the year, and on Sunday... to have many of our remote workers travel to Addgene's headquarters in Watertown, Massachusetts, for ...enjoying the party. There are so many teams that make Addgene functional, handling everything from facilities...  Throughout the party, we shared memories of Addgene's past. Framed photos and slideshows shared some...including an album of Addgenie pet photos. The famous Addgene jacket, home to a questionable collection of memorabilia...our co-ops to our directors, we can't celebrate Addgene without recognizing our incredible depositors and...

... also modify other properties such as endogenous gene expression, which can lead to changes in normal ...neuronal firing. What is chemogenetics? Chemogenetics relies on genetically introducing engineered receptors... overview of the chemogenetics system. Why use chemogenetics Because chemogenetic receptors are activated...neuroscience Resources on addgene.org Learn more about chemogenetics in our chemogenetics guide Find viral preps...equipped using fast-acting optogenetic tools. The advantages provided by chemogenetics are instead that it is...longer-lasting effects. Building your chemogenetic system To set up a chemogenetic system, you’ll need to choose...most common target for chemogenetic manipulation are neurons where chemogenetic receptors can be used ...

...Addgene is excited to announce the launch of our partnership with the Institute for Protein Innovation... of integrin antibodies, will be available on Addgene’s website this fall. IPI antibodies will come ... antibody sequences, in line with the rest of Addgene’s recombinant antibody collection, as well as access...developed to support use of their antibody tools. Addgene also offers a growing collection of educational...

...collaboration.  In that post, we talked about Addgene’s data processing needs, our criteria for selecting...Recently, Seqera published a case study on how Addgene has used Nextflow to improve and redesign our pipeline...

...everything else we do! The goal of Addgene’s educational content Addgene truly believes that sharing speeds...you ever wondered how we choose topics for the Addgene blog? Or why we make certain videos? In this post...You probably won’t be surprised to learn that Addgene’s mission statement extends beyond plasmids, AAVs...topics. What better space to do that than in the Addgene blog?  Fig. 1: One of our favorite content...content collections is our Help Center, help.addgene.org, where you can easily search articles answering ...sure things are clear, accurate, and in line with Addgene style.        Fig. 2: Reference image (...already comes from you and the questions you ask Addgene and your research community! We work to provide...

...HIV gag, pol, rev, and tet genes. It can be used with 2nd or 3rd generation lentiviral vectors and an ...3rd generation lentiviral packaging plasmid from the Trono lab that contains the gag and pol genes. It...packaging plasmid from the Trono lab contains the rev gene. It’s meant to be used with pMDLg/pRRE and the envelope...doxycycline to the grown media resulting in target gene knockdown. Note: this plasmid was called pLKO-Tet-On... 15 plasmids requested from Addgene psPAX2  - psPAX2 is a 2nd generation lentiviral packaging plasmid...their own or through Addgene!) pX330-U6-Chimeric_BB-CBh-hSpCas9 - The S. pyogenes Cas9 (SpCas9) has been...plasmid predates Addgene’s founding. Find pCMV-VSV-G. pRSV-Rev - This 3rd generation lentiviral packaging...

... the mutagenesis reaction. Alternative approaches to site directed mutagenesis Point-mutagenesis is fairly...relevant in this age of CRISPR; site-directed mutagenesis generally applies to plasmids, but may also facilitate...modifications.   Site-directed mutagenesis of Plasmids. A) Generation of mutagenized plasmids. PCR primers...also generates 4 bp overhangs. You can then use the Klenow fragment of DNA polymerase I to generate blunt...Series Use CRISPR to Generate Specific Genomic Mutations Resources on Addgene.org Browse Our Molecular...Laursen from Cornell University. Site directed mutagenesis is a highly versatile technique that can be used...analysis of proteins (to perform alanine scanning mutagenesis or targeted substitution of key residues), and...

...work with fluorescent proteins, synthetic biology, gene cloning and expression, as well as guide pages for...plasmids made in your lab, see www.addgene.org/deposit or email deposit@addgene.org for more information or ...Addgene will be at the American Society for Microbiology Microbe meeting here in Boston next week - be... and say hello! Just in time for this meeting, Addgene is launching a new resource page for the Microbiology...organisms we should include by emailing us at help@addgene.org. If you are looking for plasmids used in E. .... coli and yeast, you’re in luck! Addgene has so many of these resources that we created filters just ...microbial technology, sign up to write about it on the Addgene blog. ...