...version of PubMed.  Key features This new version has an improved search algorithm which was trained on...your search terms. Key features Semantic Scholar has several ways to focus your search results. A bar ...paper’s entry, the number of citations that paper has is displayed at the top of the page, as well as the...

...the idea that DNA methylation is linked to disease has now become much like the 1980s song “Don’t Stop Believin...none existed. Recently, however, a particular tool has gained widespread popularity – and notoriety – as...may later be excised by Cre recombinase. Addgene has several great options: pLV hUbC-dCas9-T2A-GFP for...

...a look at the secondary antibodies that your lab has on hand and choose a primary antibody that will work...secondary antibody pairing such that each target has a unique species or isotype that can be probed with...polyclonal primary antibodies.  I hope this post has explained when and why antibody isotypes matter and...

...Cas12a. This is in part due to the fact that it only has a single active site. The RuvC active site in Casɸ...rely on multiple active sites. In addition, Casɸ has a minimal PAM requirement. For instance, for Casɸ...library here A new SaCas9 ABE variant (microABEI744) has improved on-target editing efficiency and reduced...

... a raise in salary, particularly given that this has precipitated increases to the NIH recommended pay...both of these changes share a common thread that has the potential to profoundly shift the academic science...rather than as trainees in a special category, as has been the ‘labor-of-love’ philosophy of academic science...

...Epigenetics has recently been hitting the headlines, with sotires like the potential devastation of the...them. Global hypomethylation, on the other hand, has also been implicated in the development and progression...difficult to overcome until now. Alice Ting’s lab has developed a new, plasmid-based biosensor to visualise...

...    In the video above, Channelrhodopsin 2 (Chr2) has been expressed in the motor cortex of a mouse. Upon...appropriate wavelength to stimulate your opsin. Each opsin has an excitation spectrum (use the resources discussed...controlled. As discussed above, each opsin variant has slightly different activation/inactivation kinetics...

...your supervisor’s expectations Every supervisor has their own management style. A hands-on supervisor...agree with all of your ideas, but he or she probably has reasons for it. Do not take criticism personally ... of Finish Your Thesis, an online community that has supported thousands of graduate students since 2009...

... Our Editor's Choice Blog Posts The Addgene Blog has had quite the up-tick in views over the month of ...recommend taking a look at all of our posts as each one has something great to offer, I just need to get back...

...facilities, tissue culture labs and every cell culturist has to deal with this problem. It is estimated that mycoplasma...this induces a noticeable color change. This method has high sensitivity but low specificity (Degeling, 2012...affect the cell growth and viability. If mycoplasma has made permanent damage to the cell culture it is irreparable...Institute of Biosciences and Technology in India. Jadhav has a Master's degree in Molecular Biology (University...

...top of the resolving gel. A stacking gel typically has a different ionic strength and lower pH and acrylamide...Choosing your primary antibody Once the membrane has been blocked, it is stained with a primary antibody...the vendor’s website and choose an antibody that has been validated for immunoblotting. Different immunoassays..., cytoplasm and nucleus.  We hope that this blog has provided you with a good overview of the western ...

...developed for use in mammalian cells, this system has also been implemented in a variety of other organisms...Drosophila cells. Work by Wei et al. from Kang Shen’s lab has also shown that the Q system is also functional in...elegans. Subsequent work by the Luo and Potter labs has refined the Q system and introduced the second generation...multiple genes in synthetic systems. Recent work has even used all 3 in a single modular vector for ease...

...the group? Has it grown over time? If not, why not? It may not be a bad thing if the lab has stayed that...purpose, but it could also be a sign that the lab head has trouble attracting scientists. To get a better idea...intervenes if there are interpersonal conflicts PI has no idea who is dating whom Expanding your skillset...

...last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the genome engineering world by storm owing...that regulate a desired phenotype. In fact, CRISPR has already been used to engineer the genome of many ...to protoplast cells (plant cells whose cell wall has been removed). Another common delivery method is ...developed for plants and prime editing for plants has been developed for rice by Yiping Qi’s lab and for...

...sharing plasmids has become simplified through Addgene. Looking back, Addgene’s journey has been a tough ...

...Hack Day lunches These days everyone is busy and has a To-Do list long enough that there is no hope of...Cross-team “Random Lunches” With more employees, Addgene has naturally divided into smaller functional teams. ...

...DRSC/TRiP- Functional Genomics Resources website has in vitro reagents and tools to help you knock down...tools. The Vienna Drosophila Resource Center also has collections of flies for in vivo gene knock down ...finishing his immunology PhD at Harvard University. He has had nearly a decade of experience working with Drosophila...

...interference (CRISPRi) has been limited to lab-adapted bacteria because it has been challenging to introduce...the bacterial Tn7 transposon system. This system has two parts: a plasmid expressing the Tn7 transposition...

...looked hard at existing tools. As has been written here on Addgene, there has been a Cambrian-like explosion...high-throughput design and analysis of CRISPR experiments. It has certainly boosted our productivity in the Biohub....

...methods for CRISPR activation, research in the area has continued to develop. These newer methods have not... of rearranging chromatin structure. This method has shown higher activation levels than SAM in some loci...-HSF activation domains used in SAM. This method has been shown to activate genes at 2- to 3-fold higher...