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We narrowed to 878 results for: IND

Showing: 261 - 280 of 878 results

  1. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    Published
    June 17, 2021, 1:17 p.m.
    ... can find all the plasmids deposited from Di Ventura's lab, including the popular light-inducible nuclear... is about a new tool called BLADE (blue light-inducible AraC dimers in E. coli) that turns expression ...promoter systems, head over to our Plasmids 101: Inducible Promoters article.) Modifying AraC to create BLADE...exposure to blue light (Fig. 1). Another blue light-inducible dimerization domain, Vaucheria frigida aureochrome1...tested different linkers between VVD and the DNA-binding domain of AraC using a mCherry reporter downstream...Adam." This bacteriograph was created using 160 individual images stitched together. Image from Romano et...resources on the Addgene blog Learn more about inducible promoters Visit the Plasmids topic overview page...

  2. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...entails. Despite being engrossed by the theory and findings of brain science, my curiosity did not ultimately...communication. The first year of the course involved a whirlwind of skill acquisition. Classes covered filmmaking... would have a high level of career satisfaction indeed.   For many venturing forth from tertiary study...discernible paths. The first leads into the world of industry. Along this road, science communication graduates...Moreover, this subject allowed me to engage in findings relevant to my background (neuroimaging, biomechanics... a survey instrument designed to measure how individuals respond to issues of unsettled science. Understanding...building, each day brushing past and chatting with individuals whose research ranged from wildlife conservation...

  3. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ... together teams of individuals who enjoy and are experts in each of the individual tasks required to develop... but through the coordinated efforts of many individuals. Check out Joanne's Reddit AMA   Benefits of...each component of the project completed by an individual who is a local expert in that task (or has the...outcomes clear? If you don’t have clear outcomes in mind, you won’t be able to communicate these well to ...articulate the desired outcomes. Begin with the end in mind. Specify desired results. Are you delegating to...Set Concrete Goals with Your Mentor through an Individual Development Plan Resources on the Addgene Website...Website Are You a Molecular Biologist? Find Plasmids for Your Research Here!        ...

  4. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...expression, turning transcription on and off by binding proximal to their target genes. Since gene expression...), barcoded for tractability. These ORFs are individually overexpressed and available for lentiviral packaging...large sequencing endeavor, a map of possible TF-induced cellular states was generated (Joung et al., 2023...association of the uncharacterized TFs is a useful finding. Transcriptional program changes from this screen...identify both up- and down-regulated pathways. Individual cellular fates were then matched with candidate...pooled library. Louvain community clusters of individual cells along with color coded nominated reference...fluorescent controls. Alternatively, you can order individual plasmids from the library to further investigate...

  5. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    Published
    Nov. 6, 2015, 3:30 p.m.
    ...instance there are few resources to help get individuals started on the path to internationalization and...development and training in a globalized world where individuals can exchange ideas, network, collaborate, and... supportive and protective environment where individuals are empowered to productively navigate through...others have faced yet, still, this challenge did not hinder them from advancing their careers and achieving...skills, positive disposition and motivation, open mindedness, and the ability to accept feedback and adapt...to 78 undergraduate/graduate students from 5 independent universities in Spain through 22 international...Country. Mentees represented a diverse group of individuals with technical and career path needs, and came...

  6. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ...implemented and how they’ll make it easier for you to find the plasmids you need. We'll be monitoring and improving...and effective changes will give you the ability to find the plasmid with the features you need quickly. ...details. Click on the “View all sequences” button to find all available sequence information from Addgene ...hover over the ampicillin resistance gene, you’ll find the full name of the gene, the name of its gene ...functionality. Restriction Enzymes Here you'll find a table of restriction enzymes that cut the given... times they cut. Use the filters on the right to find single, dual, and 3+ cutters. You can also sort ...feature names, locations, sizes, colors used to indicate them on the map, direction (if relevant), and ...

  7. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ... All Our Fluorescent Protein Resources Find FRET Plasmids Find Empty Backbones with Fluorescent Protein...Fluorescent imaging techniques have become indispensable tools for molecular and cell biologists over...sensor displaying a 500% signal change upon calcium binding. Suzuki et al. also envision eNLs as good reporter...used a linear unmixing algorithm to decode the individual colors. The development of this algorithm is ... (2012): 1262. PMID: 23232392 PMCID: PMC3535334 Find plasmids from this paper at Addgene. Takai, Akira...2015): 4352-6. PMID: 25831507. PMCID: PMC4394297 Find plasmids from this paper at Addgene. Suzuki, Kazushi...(2016): 13718. PMID: 27966527 PMCID: PMC5171807 Find plasmids from this paper at Addgene. Additional...

  8. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    Published
    July 10, 2014, 7:17 p.m.
    ... initially include a visionary, a science-minded individual, and a specialist. Notably, the team must ... Bruce Booth How to Find a Job in Biotech and Resume/CV tips - LabSpaces Blog Find a Buisness Plan Competition...into a startup company. However, like many like-minded people, I quickly realize that a company requires...transitioned away from the bench and became all about finding the best product-market fit for this set of technologies...is. While communication styles in academia and industry vary drastically, he reassures that one can learn...anywhere. The team should initially consist of 2-3 individuals that are aligned in vision and outcomes. The ...

  9. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    .... If you are interested in reading more, you can find the rest of the Plasmids 101 posts here. Now that...β-galactosidase. Expression of the lac operon is induced by lactose, and also by a lactose analogue, IPTG...thiogalactopyranoside). (To be completely accurate, IPTG binds and inactivates the lac operon repressor, thereby...dye-linked substrate called x-gal (5-bromo-4-chloro-3-indolyl-β-D-galacto-pyranoside) into galactose and an insoluble... insoluble blue pigment (4-chloro-3-brom-indigo). So far, so good, but how does this help us screen plasmids...insert. All colonies on this plate should be blue, indicating that your IPTG and x-gal are working as they ...of false positives: Blue-white screening only indicates the presence of AN insert, not necessarily YOUR...

  10. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type
    Blog Post
    Published
    June 6, 2019, 12:41 p.m.
    ...deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like...of proteins (iLID and SspB) that undergo light-induced dimerization. Upon application of blue light, iLID...a conformational change allowing high affinity binding to SspB, brings together the split botulinum neurotoxin...that it can alter motor responses in C. elegans. Find the split BoTN/B plasmids at Addgene. Liu Q et al...segment In recent years, scientists are beginning to find connections between the microbiome and diseases,...systems that are potentially problematic for imaging. Find the ARG plasmids at Addgene. For more details on...multi-input cell classifiers in one assembly step. Find the miRNA reporter collection at Addgene! Gam JJ...

  11. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...optimized with zebrafish in mind, it can easily be applied to other systems.    Find BLITZ Plasmids here! Xiong...deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like...deposit please sign up here. Here's what you'll find in this post: Improving flexibility for in vivo...GFP-tagged version of a protein of interest. The binding of the GFP nanobody to its target stabilizes the...mouse. Image from Goertsen D, et al., 2022.  Find M-CREATE plasmids here!  Goertsen D, et al. Nature...microbial SSAPs. Image from Wang, et al, 2022.  Find dCas9-SSAP plasmids here! Wang et al. Nat Cell Biol... (TRE) or kinase translocation reporters (KTRs). Find visual barcode plasmids here! Kaufman, et al. 2022...

  12. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ..., you may be curious about all of the different kinds of antibodies that are available. One common type..., a limiting dilution is performed to isolate individual hybridomas and ELISA is used to identify the ...useful when you want to ensure that the antibody binds to a specific region of your protein of interest... example, if you are studying a protein that may bind a ligand during your experiment, then you’d want...that recognizes an epitope far from the ligand binding site (Mould et al., 2016). If your antibody recognizes... monoclonal antibodies will exhibit off-target binding to unintended proteins. This is because monoclonal...between lots over time. Specifically, the antigen-binding sites of the antibody - the paratopes - may change...

  13. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    Published
    May 3, 2018, 1:48 p.m.
    ...nicknamed CasRx), as well as EsCas13d. Find the Yan et al. plasmids here Find the Konermann et al. Plasmids here...Cas13d orthologs described in these papers can independently process their own CRISPR arrays into guide RNAs...cleavage is retained in dCas13d and is thus HEPN-independent. These enzymes also do not require a protospacer...RspCas13d. RspWYL1 also increased EsCas13d activity, indicating that WYL domain-containing proteins may be broader...even when guides were tiled across a target RNA, indicating that this enzyme does not cleave at a predictable...Since catalytically dead dCasRx maintains its RNA-binding properties, Konermann et al. tested its ability...C2c2) Characterization Resources on Addgene.org: Find RNA Knockdown Plasmids Check out our CRISPR Guide...

  14. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ...with GFP Resources at Addgene.org Find KTR reporter plasmids Find photoactivatable fluorescent proteins...and MEK1 is active or “on.” Dronpa’s color thus indicates MEK1’s current state (Green when off, dark when...endogenous ERK, and its localization serves as an indicator of MEK1 activity. When MEK1 is active, it phosphorylates...Researchers commonly use rectal bacterial infection to induce tail swelling in C. elegans, but activation of ...cells by 24-48 hours exposure to blue light also induced tail swelling in ~75% of worms (see below figure...photoswitchable kinases in my experiments? You can find the plasmids from Zhou et al here. In addition, ...empty backbones in the Michael Davidson Collection Find Photoswitchable Fluorescent Proteins ...

  15. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...to autophagy-inducing conditions. Rosella makes it easier to study the mechanisms behind autophagy by... cells. Autophagy (Greek for “self-eating”) is induced by a lack of nutrients and targets cytosol and ... via proteasome degradation. Autophagy is then induced and quantified by measuring the amount of radioactivity...radioactivity in the culture supernatant (this is indicative of protein degradation). To control for protein...cytosol in an inactive form unless autophagy is induced. Then non-selective macroautophagy of the cytosol...some conditions it aggregates in an autophagy-independent manner. Additionally, tracking Atg8p or LC3 doesn...fluorescence. After 4 hours of nitrogen starvation to induce autophagy, red but not green fluorescence accumulates...

  16. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...version, but if you find yourself doing your own conjugation, it is important to keep in mind that you will ... be useful. Mainly because, well, antibodies are kind of hard to see. To solve this issue, researchers...antibody conjugates in an earlier post, where you can find some additional tips for working with these tools...affinity for avidin and streptavidin, both of which can bind up to four biotin molecules and can themselves be...with significant multiplexing potential. You will find antibody-oligonucleotide conjugates used in assays...protein-protein interactions with other peptides, like an Fc-binding domain (Figure 1).  Figure 1: Examples of...). If some of those lysines are in the antigen binding region or even just nearby, then conjugation could...

  17. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...channels, the team will be able to validate their findings in mammalian cells with both human and murine ...cytokines actually get out of keratinocytes and induce their effector functions. It’s an important process... Hospital Zurich. IL-36 cytokines are a potent inducer of an autoinflammatory loop: keratinocytes both...Lin got me interested in TS when I was a newly independent faculty member studying sex differences in thoracic...thoracic aortic disease,” Prakash says. In induced pluripotent stem cells derived endothelial cells from...thirty GNB1 missense variants identified from 58 individuals with GNB1 disorder since the identification of...allow us to enhance our biosensor repository as we find ourselves lacking several crucial biosensors that...

  18. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type
    Blog Post
    Published
    Feb. 2, 2018, 3:20 p.m.
    ...You need a lot of time to let you mind process things. Sometimes finding that uninterrupted time to be creative..., but started looking for a new job because “it kinda sucked to work remotely. I was also tired of the...integrated and part of a team.” It took her two years to find her current position at Fred Hutch because her skill...through the tough assignments, the skills that Susan  finds important for her work include: “Listening. Evaluating...advice for getting started in science communication: “Find as many opportunities as you can to get your name...unpaid work as a student. For post-grad school individuals, Susan recommends starting a blog, but with one...internship. Application information can be found here Find tips for using social media as a scientist here ...

  19. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...standardized validation techniques for academia, industry, and especially the clinic. Popular validation...enhancing confidence and reproducibility of a study’s findings. Table 1: Biased Genotyping Options Technique... Figure 1: Schematic representations of the indicated unbiased genotyping options. Figure reproduced...experiments Unbiased detection methods are excellent for finding evidence of DSBs throughout the genome. However...due to >3 bp mismatches or that are sequence-independent are rare, but they are detectable using just ... et al. "High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells." Nature...high-throughput screening strategy for detecting CRISPR-Cas9 induced mutations using next-generation sequencing." BMC...

  20. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...preliminary data to establish your own lab and get an independent investigator grant (e.g. an R01 from the NIH,...makes time for you, as needed, this is a strong indicator that this person cares about your science and ...the reagents and outcomes. This is preferable to finding out you've been doing something all wrong or that... as they can result in spurious yet intriguing findings that fail to replicate. Plan experiments logically...great discoveries in science arose from surprising findings when studying a seemingly unrelated problem. However...As you get better at telling your story, you can find clearer ways to consolidate or present your data...you give your talk or poster to enough different kinds of audiences and if you pay attention to how the...
Showing: 261 - 280 of 878 results