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We narrowed to 791 results for: Kin

Showing: 261 - 280 of 791 results

  1. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ... used for protein localization, isolation, and tracking experiments (Rizzo, 2009). With many different...aggregation and cross-react with anti-GFP antibodies, making them difficult to use in a multicolor/multichannel...fluorophore-labeled antibodies with very specific reactivities making them more useful for multicolor imaging experiments...Davidson, and David W. Piston. "Fluorescent protein tracking and detection: fluorescent protein structure and...Blog Rabies and neuronal tracing Retrograde AAV: Making the journey from axon to nucleus AAVs CREATEd for...

  2. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...cargo Optogenetic proximity labeling and TransitID Tracking cellular history with protein ticker tape Teaching...effective and with no additional off-target edits, making them ideal for size-limited AAV delivery. PE6c ..., et al. (2023). Dynamic mapping of proteome trafficking within and between living cells by TransitID.... bioRxiv – doi: 10.1101/2023.02.07.527548.    Tracking cellular history with protein ticker tape By Brian...call for thin sections of tissue, the Wu Lab is working to create a three-dimensional atlas of protein ...

  3. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...unrelated host species, even from completely different kingdoms of life. That’s convenient for researchers who...example, puromycin inhibits protein synthesis across kingdoms by targeting a shared aspect of ribosomal function... Zeocin, a DNA intercalator, which affects all kingdoms of life. Of course, that includes you. Besides...plasmids are those that can replicate in multiple kinds of bacteria, including many natural plasmids. By...vectors can help you hop, skip, and jump between all kingdoms of life. A bit of careful design will help make...

  4. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...amazing 111 countries (Figure 1)! But while we were making the materials available to many countries, import...stays in transit for a certain number of days and tracking average transit time for each courier we contracted... to increase accessibility to our repository by making it easier to find materials and learn about them...information are truly accessible, not just available.   Looking forward Over the past twenty years, Addgene has... accessible to scientists across the globe?” By asking this question, and by listening to the answers ...

  5. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...make sure your plasmid has a promoter suited to working in your host organism. Because transcription machinery... and there is very little crossover. Generally speaking, promoters in bacteria are less diverse and complex...elements, insulators, and silencers; however, some "leakiness" of transcription may occur. This is normally ...expression mRNA Mammalian promoter from phosphoglycerate kinase gene.  Constitutive  Widespread expression, but...optogenetics mRNA  Ca2+/calmodulin-dependent protein kinase II promoter Specific Used for neuronal/CNS expression...

  6. Welcome to the Lab Bench! New Protocol Series Helps Trainees Get Started in the Lab

    Type
    Blog Post
    Published
    Sept. 7, 2021, 1:15 p.m.
    ...New to the lab? Or looking for ways to supplement your teaching? You’re in luck! Addgene’s Intro to the... We had many protocols for plasmid cloning and working with viral vectors, but what we lacked were videos...guiding completely new members of the lab or those taking introductory lab courses. The Intro to the Lab...science techniques. It’s also great for teachers looking to supplement their curriculum. From the details...

  7. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...express multiple integrins that bind the same ligand, making it difficult to dissect the function of one integrin...antibody is extremely rare. While many function-blocking integrin antibodies exist, almost all bind outside...Springer lab, reported the binding specificity, kinetics, and affinity of these 11 newly discovered synthetic...to the Springer lab, a highly specific function-blocking antibody, αVβ3.13, may also block function of ...important integrin on these cells. What’s next IPI is working with Addgene to accelerate biomedical research ...

  8. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...the specific Rho GTPase/GEF pair (Ridley, 2016). Taking control of a RhoGEF is a great way to stimulate..., 2023). Rab family proteins support vesicle trafficking and organelle dynamics, but with dozens of family...Optogenetic inhibition of Rab protein vesicle trafficking via CRY2-CIB1 clustering. Image adapted from ...chemical composition of the membrane itself? The Baskin Lab previously published OptoPLD, an optogenetically....2015.08.005.  Tei, R., Bagde, S. R., Fromme, J. C., & Baskin, J. M. (2023). Activity-based directed evolution...

  9. Enabling high school research at the Journal of Emerging Investigators

    Type
    Blog Post
    Published
    Sept. 5, 2018, 12:01 p.m.
    ...protein required for cytokinesis in JEI. Developing an interested in biology, working in a lab for the first...(the importance of word of mouth and personal networking!) and the manuscript review/submission process... first time, and thinking about the future. Many thanks to our guests,Olivia Ho-Shing, Mark Springel,...

  10. Addgene’s GIPHY Page is Here to Help You Express Yourself (and Your Gene of Interest)

    Type
    Blog Post
    Published
    Aug. 17, 2021, 1:15 p.m.
    ...We’re making some moves over here at Addgene...and they’re looping? What’s the deal with looping...GIFs? We have answers! via GIPHY   We’ve been working hard to create the most informative and entertaining...protocol videos. via GIPHY   If you’re also looking for that perfect GIF for your TikTok or Instagram...us know on Twitter @addgene! Addgene is always looking for new ways to help you celebrate those wins in...

  11. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    Published
    Oct. 31, 2024, 1:15 p.m.
    ...If you’re looking for an antibody that works for your experiment, you may be overwhelmed by options —... — or not know where to start looking to find one. Luckily, there are websites that can help: Antibody...antibody catalogs at the same time.  If you're looking for an antibody, especially a validated antibody...antibody quality throughout research, consider checking out the Only Good Antibodies community on LinkedIn...

  12. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    Published
    Jan. 15, 2019, 2:19 p.m.
    ...always gets done this time of year 😁 we stopped working on our reports/lab stuff for a few minutes to create...ladies #research #cardiovascularresearch #parkinsonsdisease A post shared by Ladies in labcoats (@ladiesinlabcoats...lab went all out to transform their lab into a walking tour of Buddy the Elf’s journey from the North ...fireplace scene.  Got our @EmoryChem lab office looking festive 🎄🌟🎁 @Addgene #DeckTheLab pic.twitter.com...

  13. Addgene’s Blog Gets a New Look!

    Type
    Blog Post
    Published
    Jan. 25, 2021, 5:54 p.m.
    ...material so that you can more easily find what you’re looking for. Whether you’re joining us for the first time...the blog that will give you a better experience. Looking for something? Use the navigation menu. The new... the top of the screen and type in what you’re looking for. After you search, you’ll see the blog articles...bloggers Like a blog post from a certain author and looking for more from them? Check out the new author pages...

  14. Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana

    Type
    Blog Post
    Published
    Dec. 20, 2016, 3:30 p.m.
    ...Seeding Labs Plasmid Grant. For this grant, we’re working with fellow nonprofit, Seeding Labs, to distribute... health. 40:17 - 44:14 : Microbiome research - working with bacterial communities both on the human body...microbiome expertise. Many thanks to Kwabena Duedu for taking the time to sit down and talk with us.  Kwabena...the Addgene, Seeding Labs Plasmid grant and is working on a variety of projects in Ghana.     Special ...

  15. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...competent lentiviruses (RCL) especially if you are working on HIV positive human cell from the clinic. The...extra care has to be taken especially if you are working with proto-oncogenes or with human samples that... is now used as it shows rapid gene expression kinetics as compared to the Tet-off system. One drawback...one with either tTA or rtTA (FUW-M2rtTA). In this kind of approach, a population of cells might express...transgene resulting in low inducibility and potentially making it difficult to interpret your results. This can...

  16. Synthetic Biology, Artificial Chromosomes, iGEM & More - Interview with Tom Ellis

    Type
    Blog Post
    Published
    Nov. 29, 2017, 2 p.m.
    ...a startup and back to academia. Chapter 1: How working at a startup prepared Tom for an academic career...more complicated biological devices. Chapter 6: - Making research go faster through Open Access. Chapter...investigate synthetic biology at Boston University, working in one of the founding groups of the field under... Plasmids 101: Golden Gate Cloning Learn About Working at a SynBio Startup in Our Interview with Michael...

  17. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    Published
    Oct. 6, 2016, 2:30 p.m.
    ...when did you start thinking about them? 18:30 - 19:54 : How to think about picking a post-doc project ... you leverage your community, think hard about picking a good mentor, and begin considering career options...Mentoring" We'd like to thank Niroshi and Ben for taking the time to sit down and talk to us and wish them...

  18. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    Published
    Nov. 13, 2015, 3:30 p.m.
    ... including that it’s never too early to start networking! Learning about careers at Addgene Afterwards...popular, with the students excited to see a real working lab full of busy Addgenies preparing plasmid samples...impressed our guests, as did how much work is put into making sure scientists all over the world know that we...Advice from Our Bloggers Learn How to Improve Your Networking Skills  ...

  19. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    Published
    March 26, 2015, 3:24 p.m.
    ...providing quality control and customer support, making plasmids easy for you to find and request in our... Addgene? Here's an inside peak into the daily workings in Addgene's lab. We ship individual plasmids ...Stabs are organized by shipment and moved to our packing area.Using the barcode system, the correct plasmids...necessitate an office move. We've got ~50 Addgenies working to ship plasmids, analyze QC sequence, answer scientists...

  20. Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors

    Type
    Blog Post
    Published
    March 17, 2021, 2:40 p.m.
    ...Brownstein, an Addgene board member. When I was working in Human Resources at Harvard Medical School, Joanna...Joanna was my colleague. She mentioned Addgene was looking for someone with HR expertise to join their newly... her forever home. I feel blessed that I’m not working so that I’m available to spend time caring for ...for my mother. For fun, I enjoy pilates, jewelry making, reading, sailing, and gardening. Learn more about...
Showing: 261 - 280 of 791 results