We narrowed to 317 results for: MUT

1. Plasmids 101: Knockout/Knock-In Plasmids

   Type

   Blog Post

   Published

   Dec. 1, 2016, 3:30 p.m.

   ...possible to insert, or knock-in, any gene, tag, or mutated exon into the genome. For this purpose, the sequence...

2. Google Forums Round Up: First Impressions of NgAgo

   Type

   Blog Post

   Published

   Aug. 4, 2016, 2:30 p.m.

   ...wouldn’t be a surprise if someone came up with a mutant NgAgo (or a novel endonuclease) protein that can...

3. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

   Type

   Blog Post

   Published

   April 19, 2016, 2:30 p.m.

   ... and Citrine) can be monomerized with an A206K mutation [2]. Addgene has many of these constructs and ...

4. Anatomy of a Plasmid Page at Addgene

   Type

   Blog Post

   Published

   Feb. 4, 2016, 3:30 p.m.

   ...depositor has indicated that there is a particular mutation found within the gene/insert, this information...

5. Plasmids 101: Multicistronic Vectors

   Type

   Blog Post

   Published

   Sept. 9, 2014, 8:20 p.m.

   ...should also note that IRES and 2A peptides are not mutually exclusive elements. Labs have successfully utilized...

6. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

   Type

   Blog Post

   Published

   June 12, 2014, 3:30 p.m.

   ...tools, double dimerization GFPs, and circular permutation GFPs. Hopefully we will have new tools for plant...

7. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

   Type

   Blog Post

   Published

   Dec. 12, 2013, 3:35 p.m.

   ...gates." Science. 340, 599-603 (3 May 2013). Vivek Mutalik et al. "Precise and reliable gene expression via...

8. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

   Type

   Blog Post

   Published

   Dec. 7, 2020, 2:53 p.m.

   ...adapted for a wide variety of applications - as mutagenesis tools for genetic studies (e.g. using mariner...

9. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

   Type

   Blog Post

   Published

   July 16, 2020, 1:15 p.m.

   ...autoplay=False, hidden_controls=False, loop=False, muted=False, width='600', height='189', player_id='31668202421...

10. Tips for arabidopsis transformation

    Type

    Blog Post

    Published

    Oct. 25, 2018, 1:23 p.m.

    ...and proteins in living plants, to complementing mutant phenotypes. Arabidopsis is amenable to the floral...

11. Viral Vectors 101: The Retroviral Lifecycle

    Type

    Blog Post

    Published

    July 27, 2023, 1:15 p.m.

    ...namely that they can cause cancer via insertional mutagenesis (which HIV does not do). Furthermore, molecular...

12. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type

    Blog Post

    Published

    Sept. 28, 2017, 2:01 p.m.

    ...Targeted Evolution. In this technique, a pool of mutant AAV capsid (cap) expressing plasmids is generated...

13. Transferable Skills Guide: Identifying Your Transferable Skills

    Type

    Blog Post

    Published

    Jan. 11, 2018, 3:29 p.m.

    ...lucky socks, persevered through a nerve wracking commute complete with a disabled train, and now you’re ...

14. Lentiviral Vector Uses and Overview

    Type

    Blog Post

    Published

    May 19, 2016, 2:30 p.m.

    ...transfer plasmids. The packaging plasmid carried a mutated HIV-1 provirus that couldn’t package itself due...

15. CRISPR Antimicrobials

    Type

    Blog Post

    Published

    May 3, 2016, 2:30 p.m.

    ...defective phagemid; Bikard et al. did not observe any mutations at the target site. In a mouse skin colonization...

16. Plasmids 101: Protein tags

    Type

    Blog Post

    Published

    Dec. 11, 2014, 4:26 p.m.

    ...column runs. The TEV protease itself, with various mutations used to increase its stability activity, can be...

17. Lab to Office Culture Shock

    Type

    Blog Post

    Published

    Feb. 5, 2019, 1:04 p.m.

    ...everyone has a voice and the group can come to a mutual agreement is an amazing thing to be a part of and...

18. Transferable Skills Guide: Creativity

    Type

    Blog Post

    Published

    Jan. 18, 2022, 2:15 p.m.

    ...Gather the resources you’ll need ahead of time and mute your notifications during. (Boyes, 2019) If you’...

19. Hot Plasmids - August 2020

    Type

    Blog Post

    Published

    Aug. 18, 2020, 1:15 p.m.

    ...) and GRAB_NE1m (medium affinity), as well as GRAB_NEmut as a negative control. Find the sensors at Addgene...

20. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type

    Blog Post

    Published

    Nov. 1, 2018, 12:35 p.m.

    ... a GFP cassette, and introduced site-directed mutations, all without introducing unwanted selection markers...