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We narrowed to 676 results for: Mos;

Showing: 261 - 280 of 676 results

  1. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    Published
    July 8, 2025, 1:15 p.m.
    ...Planning makes perfect Our first tip, and arguably the most important, is to take some time to sit down, plan...assemblies in silico before you start doing your cloning. Most sequence analysis software includes Golden Gate ...kit has just arrived! MoClo kits from Addgene will most likely arrive in your laboratory as plates of frozen...

  2. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...adenine transversion editors Uracil excision is the most common type of base excision repair. But mammals... retained considerable A → G editing activity at most sites, in addition to A → C and A → T activity. ... base from DNA to create an abasic site. Like in most other base editor designs, the Cas9 nicks the non-edited...

  3. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    Published
    Sept. 13, 2018, 1:55 p.m.
    ... exist for reducing Cas9 off-target effects, but most studies have focused on the off-target cutting of...not associated with ricin regulation, including almost all genes involved in the production of a cell ...fail to identify some known ricin-regulators, but most of these genes are also essential for growth and...

  4. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...much easier to map these neuronal networks. For almost three decades, recombinant Adeno-associated virus...IHC) experiments with tag-specific antibodies. The most famous ones are influenza hemagglutinin (HA), myelocytomatosis... microscopes, but they can also be used with the most cutting-edge imaging technologies such as array ...

  5. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...your best to show up as your most prepared, can-do self and make the most of it! So, what can you do ...dynamic and set yourself up for success! Getting the most out of the training experience In research there...

  6. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    Published
    April 2, 2019, 1:44 p.m.
    ...the fly community. Not surprising, this is where most of our conference room names originated from. Let...that contain SHH, you can find them all here! The most popular SHH plasmid however was deposited by none...insert specific pieces of DNA into vertebrate chromosomes via a cut and paste mechanism. Scientists reconstructed...

  7. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ... just creating, which for many scientists is the most enjoyable aspect of their chosen career. You can...’t know what it’s like to be properly onboarded. Most work environments outside of academia provide a ...skills you pick up wherever you work, and you’ve almost certainly developed some in the lab whether you...

  8. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...markers are set up sequentially, from broadest to most specific marker, and are represented as a series...quadrant will often be too clean for real-world data. Most plots will have a shape, like a box, ellipse, or...  As the gates progress from broadest markers to most specific, the first step is to separate cells from...

  9. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...engineering tool. It is easy to use, functional in most species, and has many applications. That said, SpCas9...terms of structure, Cas13a shares no homology to the most commonly used CRISPR enzymes - Cas13a contains two...specifically targeted RNAs. Finally (and perhaps most interesting), Cas13a’s propensity to cleave any ...

  10. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...fluorescent visualization techniques for decades, most notably for fluorescent in situ hybridization (FISH...readout may render this approach less sensitive in most applications. Learn About Fluorescent Biosensors...Hanhui, et al. “Multicolor CRISPR labeling of chromosomal loci in human cells.” Proc. Natl. Acad. Sci. ...

  11. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...block of time was. Eventually, I learned that I was most productive in short bursts (even twenty minutes ...minutes was enough to make some progress). For most researchers, it’s not always possible to pull a Thoreau ...useful data Typically lists and pictures are the most useful parts of reviews. These could be in the form...

  12. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ... is reinforcing, the animal will prefer to spend most of its time in the chamber in which it receives ...conversely, if the circuit is aversive, it will spend most of its times in the opposite chamber) [2]. Laser...standardization of genetic tools in the laboratory mouse, most optogenetics is performed in this species. However...

  13. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ... extracellular environment and the cytoskeleton. Most integrins have three conformational states: bent-closed...many function-blocking integrin antibodies exist, almost all bind outside the ligand-binding pocket, their...challenging the long-held belief that αVβ3 would be the most functionally important integrin on these cells. ...

  14. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...Microscopy (University of Amsterdam). GFP is the most popular, most widely used genetically encoded fluorescent...maturation to functional, fluorescent protein in almost all organisms and cell types, (ii) no need to add...

  15. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...microtubule-cutting sword Microtubules are one of the most important components of the cytoskeleton. They provide...provide cells with physical structure, separate chromosomes during mitosis, support transport of organelles...adhesion But what if you’re interested in that other most-important component of the cytoskeleton – actin?...

  16. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ... the company. I’d identified the ways to get the most out the resources we have, and we developed a great...But there were still many restrictions in place, most importantly, masks.  Besides the muffled voices ....  Test: Our first official shoot went off with almost no hitches. The wheeled stand was a bit cumbersome...

  17. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ...side of things that have the most utility; i.e. those things that will be most useful for achieving your ...finish any additional work you might need to do. In most cases, I would recommend the second option for the...

  18. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...types and often results in biallelic modifications. Most commonly, DSBs are repaired by the nonhomologous...associated with dementia, such as APP and PSEN1, in most cases the intended HDR events were corrupted by ...’t get what we wanted. The Indels were gone from most HDR reads that we studied by deep sequencing, but...

  19. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...previously appreciated. PhosphoSitePlus, one of the most inclusive databases of post-translational modifications... A number of classic methods exist for this, the most common of which is the utilization of phosphomimetic...are available through Addgene, but we have worked most extensively worked with and optimized Sep-OTSλ and...

  20. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ... Sanger sequencing of pooled samples showed that most of the HIV-1 genome had been excised, leaving only...rather than the ART-induced viral control found in most HIV-1 patients. Obstacles for CRISPR HIV-1 therapy...therapeutic possibilities for some of the world’s most common diseases. References Zhang Y, Yin C, Zhang...
Showing: 261 - 280 of 676 results