We narrowed to 329 results for: NUC

1. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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   Blog Post

   Published

   April 23, 2018, 2 p.m.

   ...estradiol for hER or progesterone for hPR - leads to nuclear localization, binding to the appropriate promoter...

2. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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   Blog Post

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   Sept. 8, 2020, 2 p.m.

   ...signalling complex assembly, and localization to the nucleus,” says Dr. Terry Hébert, a professor and assistant...

3. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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   Blog Post

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   Jan. 31, 2019, 1:32 p.m.

   ...itself. They deliver compounds like proteins and nucleic acids from cancer cells to the microenvironment...

4. Design Tips for Prime Editing

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   Blog Post

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   Jan. 23, 2025, 2:15 p.m.

   ...transcriptase template, starting with about 10–16 nucleotides. For longer templates, it is even more important...

5. Making CRISPR Plasmids Using Fragmid

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   Blog Post

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   Aug. 29, 2024, 1:15 p.m.

   ...destination vector via Golden Gate cloning, perform an exonuclease cleanup, transform, plate, and pick. In short...

6. Plasmids 101: Modular Cloning

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   Blog Post

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   March 12, 2024, 1:15 p.m.

   ...depend on many gRNAs delivered with a relevant Cas nuclease. MoClo can be used to assemble multiplexed gRNA...

7. CRISPR in the Clinic

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   Blog Post

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   Nov. 2, 2023, 1:15 p.m.

   ...hATTR) hATTR is caused by a variety of single nucleotide polymorphisms (SNPs) in the TTR gene and is molecularly...

8. Viral Vectors 101: Calcium Sensors

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   Blog Post

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   Aug. 10, 2023, 1 p.m.

   ... localize at the plasma membrane. GCaMP-X is a nucleus-targeted GCaMP6 with an extra apoCaM-binding motif...

9. Popular Retroviral Vectors and Their Uses in Scientific Research

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   Blog Post

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   July 24, 2018, 12:51 p.m.

   ...Murine Stem Cell Virus, MSCV) each with a unique 6 nucleotide barcode. This collection can be used for in vivo...

10. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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    Blog Post

    Published

    May 15, 2018, 12:43 p.m.

    ...accumulation in subcellular compartments such as nuclear speckles. Furthermore, CRY2clust, when integrated...

11. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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    Blog Post

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    May 7, 2020, 1:15 p.m.

    ...motif (PAM) made up of 5′-NGG-3′ (“N” can be any nucleotide base, followed by 2 guanines). This limits the...

12. 5 Tips for Troubleshooting Viral Transductions

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    Blog Post

    Published

    Aug. 11, 2016, 2:23 p.m.

    ...integration of transfected DNA into normal human cells."Nucleic acids research 18.9 (1990): 2733-2738. PubMed PMID...

13. Tips for Titering Your Lentiviral Preps

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    Blog Post

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    March 15, 2016, 2:30 p.m.

    ...presence of a viral protein, such as p24, or viral nucleic acid. Functional titers, or infectious titers, ...

14. Illuminating Epigenetics with A FRET Based Biosensor

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    Blog Post

    Published

    Nov. 19, 2015, 3:30 p.m.

    ... of the DNA with a high frequency of C and G nucleotides). The conversion of cytosine to 5-methylcytosine...

15. Hot Plasmids: Winter 2025

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    Blog Post

    Published

    March 11, 2025, 1:15 p.m.

    ...PE2max-NC and ePEmax3 optimized for dicots. NC: nucleocapsid RNA chaperone, RT: reverse transcriptase. Right...

16. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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    Blog Post

    Published

    Oct. 11, 2018, 12:30 p.m.

    ...ligase fidelity and bias during DNA end-joining." Nucleic acids research (2018). PubMed PMID: 29741723. PubMed...

17. Plasmids 101: Protein Expression

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    Blog Post

    Published

    June 7, 2018, 1:17 p.m.

    ... include ribosomes, RNA polymerase, tRNAs, ribonucleotides and amino acids. Cell-free expression systems...

18. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    Blog Post

    Published

    June 18, 2020, 1:15 p.m.

    ... randomized with a defined set of different trinucleotides (corresponding to different amino acids) in...

19. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type

    Blog Post

    Published

    March 28, 2018, 6:52 p.m.

    ...xCas9 are similarly flexible. Like the xCas9 3.7 nuclease, xCas9 3.7 fused to third generation cytidine ...

20. Plasmids 101: Knockout/Knock-In Plasmids

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    Blog Post

    Published

    Dec. 1, 2016, 3:30 p.m.

    ...repair harmful double stranded breaks, in which nucleotide sequences are exchanged between two similar or...