We narrowed to 461 results for: PAC

...to for each sprint, to ensure we will have the capacity to finish all of the work. Since we work in iterative...sharing knowledge allows for life to happen without impacting the overall work. Each of our sprints is themed...

...recently moved to our brand new location, with more space to grow and help scientists share resources in new...segmentation during embryogenesis. Typically, regularly spaced denticles (bristles) outline the segments of the...

...colleague and give you a million dollar startup package. There are three steps to chalk talk preparation...have developed your research vision, you need to package it as a roughly 30 minute chalk talk. You will ...

...consuming and difficult. Finding volunteers who had space to shoot was another hurdle. As we slowly started...strict safety protocols to keep Addgenies safe. Space was at a premium, so I had to figure out a solution...

...mice to study how different mutations in leukemia impact different treatment regimens (Zuber et al., 2009... our own cells. Like human cells, yeast DNA is packaged into chromosomes and about 23% of yeast genes ...

...for several FRET biosensors (yellow cameleon 3.60, EPAC, and Galphai), the C-terminus and N-terminus of .... Klarenbeek, Jeffrey, et al. "Fourth-generation epac-based FRET sensors for cAMP feature exceptional ...

...of interest that are found in the extracellular space. The targeted epitopes can be motifs within transmembrane...Proliferation dyes can visualize proliferative capacity and speed of cells. (a) With each division half...

...application can benefit many others and accelerate the pace of research. We at Addgene hope to see the problem...PMCID: PMC4778041. Find CRISPR and lentiviral packaging plasmids used in this publication at Addgene. ...

...sample migrate through the stacking gel at the same pace and enter the resolving gel at the same time where...popular due to its durability and high binding capacity. In addition, PVDF membranes can be repeatedly...

...modifications to DNA or histones, the proteins that help package DNA, were associated with gene expression or silencing...particularly large protein, and this fusion allows for packaging into viral vectors and targeted delivery into ...

...Plus, the tools used here (also available as AAV Packaged on Request) will be useful for related studies...transfer increases the metabolic and metastatic capacity of cancer cells. This is certainly just the beginning...

...needed to replicate inside a host cell but with space to insert your genetic cargo of choice. After hearing...taken them for a test drive! We validated viral packaging and strong expression after inserting EGFP and...

...are composed. Fear not – the state machine design space is highly degenerate and often times there are multiple... et al. "Permanent genetic memory with> 1-byte capacity." Nature methods 11.12 (2014): 1261-1266. PubMed...

...Furthermore, piggyBac™ offers a large cargo-carrying capacity (over 200 kb has been demonstrated) with no known...certain genes, such as those that have negative impacts on fitness. One consideration when making a transgenic...

... a good story (1). From a deep sea shrimp to a compact powerhouse of bioluminescence, NLuc is 100-fold...LumiFluor reporter constructs were designed to be packaged into a retrovirus for infection of host cells....

... much as possible and the ones that are tightly packed with seals. Use disposable pipettes to avoid cross-contamination...be overcrowded (filled no more than 60% of its capacity) which can cause mycoplasma infection from one...

...other side, are too long for rAAVs, which can only package sequences shorter than 5 kb (Hirsch et al., 2016...Delivering transgenic DNA exceeding the carrying capacity of AAV vectors." Gene Therapy for Neurological...

... epigenetic modification, bulges) that may also impact off-target edits. As a result, only sequencing ...algorithms may not provide a full picture of the impact of CRISPR editing in the model cell line or organism...

...populations that are the most likely to be positively impacted by the trial. During clinical trials, expansion... strategies that synergize with these positive impacts on the immune system will likely have better treatment...

...distance in the placement of plasmid elements can impact expression levels and consequently convolute the... different lentiviral plasmid backbone elements impact viral titer and to compare different Cas enzymes...