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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 457 results for: PAC

Showing: 261 - 280 of 457 results

  1. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...of interest that are found in the extracellular space. The targeted epitopes can be motifs within transmembrane...Proliferation dyes can visualize proliferative capacity and speed of cells. (a) With each division half...

  2. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...consuming and difficult. Finding volunteers who had space to shoot was another hurdle. As we slowly started...strict safety protocols to keep Addgenies safe. Space was at a premium, so I had to figure out a solution...

  3. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...mice to study how different mutations in leukemia impact different treatment regimens (Zuber et al., 2009... our own cells. Like human cells, yeast DNA is packaged into chromosomes and about 23% of yeast genes ...

  5. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...application can benefit many others and accelerate the pace of research. We at Addgene hope to see the problem...PMCID: PMC4778041. Find CRISPR and lentiviral packaging plasmids used in this publication at Addgene. ...

  6. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...needed to replicate inside a host cell but with space to insert your genetic cargo of choice. After hearing...taken them for a test drive! We validated viral packaging and strong expression after inserting EGFP and...

  7. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...sample migrate through the stacking gel at the same pace and enter the resolving gel at the same time where...popular due to its durability and high binding capacity. In addition, PVDF membranes can be repeatedly...

  8. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...modifications to DNA or histones, the proteins that help package DNA, were associated with gene expression or silencing...particularly large protein, and this fusion allows for packaging into viral vectors and targeted delivery into ...

  9. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ... a good story (1). From a deep sea shrimp to a compact powerhouse of bioluminescence, NLuc is 100-fold...LumiFluor reporter constructs were designed to be packaged into a retrovirus for infection of host cells....

  10. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...thus frameshifts that disrupt the protein-coding capacity of a locus. When using S. pyogenes Cas9 (SpCas9.../doi.org/10.1038/s41587-020-0561-9 Dempster JM, Pacini C, Pantel S, Behan FM, Green T, Krill-Burger J,...

  11. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...are composed. Fear not – the state machine design space is highly degenerate and often times there are multiple... et al. "Permanent genetic memory with> 1-byte capacity." Nature methods 11.12 (2014): 1261-1266. PubMed...

  12. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...Furthermore, piggyBac™ offers a large cargo-carrying capacity (over 200 kb has been demonstrated) with no known...certain genes, such as those that have negative impacts on fitness. One consideration when making a transgenic...

  15. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ... epigenetic modification, bulges) that may also impact off-target edits. As a result, only sequencing ...algorithms may not provide a full picture of the impact of CRISPR editing in the model cell line or organism...

  16. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...distance in the placement of plasmid elements can impact expression levels and consequently convolute the... different lentiviral plasmid backbone elements impact viral titer and to compare different Cas enzymes...

  17. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...populations that are the most likely to be positively impacted by the trial. During clinical trials, expansion... strategies that synergize with these positive impacts on the immune system will likely have better treatment...

  18. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...implications for our public policies—many of which impact reproductive rights and justice. Whether it’s used...interests. Our marches across the world have had an impact already, gaining additional funding and the eyes...

  19. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ... formation slightly. Designing the sgRNA with a spacer that only binds the edited strand, as in the PE3b...Phage-assisted evolution and protein engineering yield compact, efficient prime editors. Cell, 186(18), 3983-4002...

  20. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...multiplex your reference genes to save on plate space. For many cell lines and tissue types, appropriate...your reference gene set can save significant plate space and may be worth the initial effort of setting it...
Showing: 261 - 280 of 457 results