We narrowed to 289 results for: Pol;

1. Overcoming the AAV Size Limitation for CRISPR Delivery

   Type

   Blog Post

   Published

   Sept. 16, 2020, 1 p.m.

   ...Split inteins are a pair of naturally occuring polypeptides that, when at the ends of two proteins, mediates...

2. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

   Type

   Blog Post

   Published

   Oct. 13, 2022, 1:45 p.m.

   ...engaging target DNA. The reverse transcriptase polymerizes DNA onto the target strand based on the sequence...

3. Plasmids 101: Stringent Regulation of Replication

   Type

   Blog Post

   Published

   June 20, 2024, 1:15 p.m.

   ... from the ori. In the elongation phase, a DNA polymerase synthesizes a new DNA strand at each replication...

4. Plasmids 101: Broad Host Range Plasmids

   Type

   Blog Post

   Published

   April 11, 2024, 1:15 p.m.

   ...structural elements, oris interact with various RNA polymerases and proteins from bacterial hosts. In a plasmid...

5. How to Be an Excellent Trainee

   Type

   Blog Post

   Published

   Sept. 26, 2023, 1:15 p.m.

   ...It’s often easiest to train individuals who are polite, appreciative, and clear about their needs. Remember...

6. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

   Type

   Blog Post

   Published

   Jan. 24, 2023, 2:15 p.m.

   ... the cells could be batch-sorted to isolate a polyclonal cell line with strong gas vesicle expression ...

7. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

   Type

   Blog Post

   Published

   Feb. 18, 2020, 2:15 p.m.

   ... Marc Sze, Victoria McGovern, and Julie Wolf. Apologies to anyone I’ve inadvertently left off this list...

8. The Challenges of Cell Culture

   Type

   Blog Post

   Published

   May 5, 2016, 2:30 p.m.

   ...whether the cell lines had isoenzymes and genetic polymorphisms specific to their origins. This technique is...

9. Plasmids 101: E. coli Strains for Protein Expression

   Type

   Blog Post

   Published

   Feb. 10, 2015, 3:06 p.m.

   ...induction system. The DE3 lysogen expresses T7 RNA polymerase (RNAP) from the bacterial genome under control...

10. Adeno-associated Viruses (AAVs) for Genome Editing

    Type

    Blog Post

    Published

    March 27, 2018, 1:32 p.m.

    ...choice. These vectors are very similar, both with polylinkers for the addition of gene-specific 1 kb homology...

11. Harnessing Bacterial Toxins for Allelic Exchange

    Type

    Blog Post

    Published

    Aug. 15, 2019, 12:30 p.m.

    ...tuner: a computer program for the construction of polylinker sequences of molecular vectors". Proceedings ...

12. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type

    Blog Post

    Published

    April 9, 2019, 1:13 p.m.

    ...Bendezú, Felipe O., et al. "Spontaneous Cdc42 polarization independent of GDI-mediated extraction and actin-based...

13. Antibodies 101: Introduction to Immunofluorescence

    Type

    Blog Post

    Published

    Nov. 16, 2021, 2:15 p.m.

    ...common is that secondary antibodies are generally polyclonal, which means that multiple secondary molecules...

14. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type

    Blog Post

    Published

    June 3, 2021, 1:15 p.m.

    ...recombinant antibody. They are ~25 kDa single polypeptides that contain the variable light chain (VL) and...

15. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type

    Blog Post

    Published

    Dec. 2, 2019, 2 p.m.

    ...mitochondrial matrix (Rhee et al., 2013) and determine the topology of the mitochondrial calcium uniporter (Martell...

16. Multiple Plasmids at a Low Price: Kits from Addgene

    Type

    Blog Post

    Published

    Sept. 14, 2016, 2:30 p.m.

    ...terminators can be used to precisely tune your polycistronic E. coli expression constructs. Some applications...

17. Using Phosphoserine to Study Protein Phosphorylation

    Type

    Blog Post

    Published

    June 23, 2016, 2:30 p.m.

    ...extension and translation of the pSer-incorporated polypeptide. These three components were shown to effectively...

18. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type

    Blog Post

    Published

    May 10, 2016, 2:30 p.m.

    ...Addgene. Kaminski R, Chen Y, Fischer T, Tedaldi E, Napoli A, Zhang Y, Karn J, Hu W, Khalili K. Elimination...

19. Is this the right place for me? 8 tactics for choosing a lab

    Type

    Blog Post

    Published

    Oct. 2, 2018, 12:56 p.m.

    ...treat one another (especially those low on the totem pole). Can you see yourself enjoying this atmosphere?...

20. Antibodies 101: Multiplex Immunofluorescence

    Type

    Blog Post

    Published

    May 23, 2023, 1:15 p.m.

    ... Kv2.1 channel anti-Kv2.1 KC (Rabbit IgG polyclonal) Goat anti-Rabbit IgG   If you need more...