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We narrowed to 610 results for: SCR

Showing: 261 - 280 of 610 results

  1. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...we probably exchanged dozens of drafts of the manuscript. This is the time to transform the document into...Resources on the Addgene Blog Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing ...Organizing Tips for Improving Your Next Manuscript 6 Tips for Grant Writing Resources on Addgene.org Check out...

  2. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...expected for postdocs in many cases should be as described in the notice of the new NRSA levels and in the...very relieved not to be going through the urgent scramble of their peer institutions. The issues facing ..., and it is becoming ever clearer that outside scrutiny of science and the academic system is increasing...

  3. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...Easi-CRISPR in vitro transcribes RNA encoding your repair template, then uses reverse transcriptase to generate..., and ultraviolet light. In this post, we will describe the mechanism of homology directed repair, one...

  4. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...strong transcriptional terminator BBa_B1002 (from IGEM) upstream of the MCS to reduce transcriptional readthrough...worked too. Rhamnose induction relies on the transcriptional activator rhaS. To allow manipulation of bacteria...

  5. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ...same fluorescent markers, Brainbow 3.1 and 3.2 (described in the same publication) are unique because by...cell type. The Confetti mouse was first used to describe the self-renewal of cells in the intestinal crypt...previous Brainbow technologies were useful for discriminating neurons within only one field of view. Tetbow...

  6. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...methyltransferase — that can drive lasting transcriptional silencing (Nuñez et al., 2021). Now, in a collaboration...versions using dCas9, zinc finger proteins, and transcription activator-like effectors (TALEs), with fifteen...Weissman, J. S. (2021). Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing. Cell...

  7. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ... The one-pot saturation mutagenesis technique described in Wrenbeck et al is a PCR-based approach for ...library that’s ready to be tested in a functional screen of your liking. This method is similar to site-directed... screen...

  8. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...site-specific incorporation of phosphoserine (as described below) provides researchers with a valuable tool...context-specific effects of UAG suppression, as described (6). To determine the stoichiometry of phospho-incorporated...phospho:non-phospho species on PhosTag gels as described above. Reagents and protocols Sep-OTSλ plasmid...

  9. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...applications, including transgenesis, gene-trap screens, and gene editing. How does the piggyBac™ system...effects, such as with signaling molecules or transcription factors, or when the gene is constitutively-..., since there is no selection, you will need to screen somewhere between one hundred and one thousand ...

  10. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...Antibody-independent methods may include analysis of transcriptomic or proteomic data from several different tissues... feasible and (2) large-scale proteomic and transcriptomic datasets already exist.   Example: You want...tissues. You know that there is a wealth of transcriptomic data from this sample type, so you use your...

  11. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...brightness of individual fluorophores. Table 1 describes the brightness of a few commonly used fluorophores...brightness levels on your smartphone or computer screen), and you should be able to properly label many...panel! Designing a Panel In this example, I will describe a basic panel (Table 2, Figure 5) for a hypothetical...

  12. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ... drug discovery by big pharma (i.e. HTRF and LanthaScreen) (Degorce et al., 2009), measuring intracellular...are critical in setting up a FRET-based assay or screen (the Addgene blog has an excellent primer on FRET...it all together The FRET donors and acceptors described above can be paired together to measure a wide...

  13. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ... by abundant transcripts, leading to falsely lowered levels for less abundant transcripts. However, adding...can even be used to detect a single copy of a transcript. But this sensitivity can sometimes cause as ...

  14. The 12 Days of CRISPR

    Type
    Blog Post
    Published
    Jan. 3, 2023, 2:15 p.m.
    ...Noninvasive assessment of gut function using transcriptional recording sentinel cells! 🎵 On the 11th day...gave to me: CRISPR activation and interference screens for primary human T cells🎵 On the 3rd day of CRISPR...

  15. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ...various CRISPR applications (such as visualize, screen, activate, and repress) on Thursday. We’d also ...from nickases to base editing to pooled library screening. It also has information for planning your CRISPR...

  16. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...used to increase or decrease expression of a gene. Screen: A library of gRNAs with a CRISPR nuclease target...cell population. The resulting mutant cells are screened for phenotypes of interest. Limitations of CRISPR...

  17. Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

    Type
    Blog Post
    Published
    May 12, 2017, 12:58 p.m.
    ...populate your “PubMed filter list” to the left of your screen. That’s it! Next time you log-in to your PubMed...Scientific Review Article Tips for Improving Your Next Manuscript Resources on Addgene.org Learn How to Order...

  18. Newly Updated AAV Data Hub!

    Type
    Blog Post
    Published
    Feb. 7, 2023, 2:15 p.m.
    ...your experiment* Functional data, if the report describes a functional vector such as a channelrhodopsin...site as well as the target region, if the report describes a retrograde serotype An abstract that summarizes...

  19. Addgene’s Blog Gets a New Look!

    Type
    Blog Post
    Published
    Jan. 25, 2021, 5:54 p.m.
    ... Just click on the search bar at the top of the screen and type in what you’re looking for. After you ...know about new articles on the Addgene blog by subscribing to instant alerts below.  ...
Showing: 261 - 280 of 610 results