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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 499 results for: SHA

Showing: 261 - 280 of 499 results

  1. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...action potential-evoked axonal glutamate signals. Shading denotes SEM. D) Summary of ΔF/F0, Time-to-peak,...limitations of existing backbones, we're excited to share our very own set of empty, HA-, and EGFP-expressing...

  2. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ...doi.org/10.1038/s41467-023-39554-7 Issa, S. S., Shaimardanova, A. A., Solovyeva, V. V., & Rizvanov, A. A. ...j.neuron.2016.09.021 Walters, R. W., Yi, S. M., Keshavjee, S., Brown, K. E., Welsh, M. J., Chiorini, J. ...

  3. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...blocking for 1 h at room temperature with gentle shaking, gently wick off excess liquid from each membrane...start) and add your membranes to the solution, shaking gently for 1 h at room temperature. This will allow...

  4. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ... model. For example, in a recent study by the Wickersham lab (Lavin, TK. 2020) it was determined that ...10.1089/hum.2019.277 Lavin TK, Jin L, Lea NE, Wickersham IR (2020) Monosynaptic Tracing Success Depends...

  5. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    Published
    Aug. 5, 2025, 1:15 p.m.
    ...course on yeast display for antibody discovery, to share their expertise on the topic. Evolving the future...with verifiable data is critical, especially when sharing with other scientists. Addgene partners with IPI...

  6. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...sequences, but are sensitive to distortions in helix shape (22). Large purine:purine mismatches and most smaller...hydrogen bonds and a base-pair size nearly indistinguishable from a Watson-Crick base pair, are joined ...

  8. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...how science can be accelerated immensely when we share knowledge and reagents. I have not intentionally...PMCID: PMC4744125. 3. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide...

  9. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...:19169259. PubMed Central PMCID:PMC2901231. 3.Verkhusha, Vladislav V., and Alexander Sorkin. "Conversion...25581799. PubMed Central PMCID:PMC4344411. 13.Berlin, Shai, et al. "Photoactivatable genetically encoded calcium...

  10. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ... focus). This means that the images will appear sharp without interference from blurry out-of-focus light... lightsheet that projects a number of thin cone-shaped beams into the sample. These beams interfere to...

  11. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ... also known as immunoglobulins, are ~150 kDa, Y-shaped proteins that are both a natural part of the immune... the Addgene blog: Read about another reagent sharing platform for antibodies, the Developmental Studies...

  13. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...and reliable qPCR data, you’ll of course want to share it so others can provide you with the highest form....2018.12.002.Teo IA; Choi JW;  Morlese J; Taylor G; Shaun S. LightCycler qPCR optimisation for low copy number...

  15. New Western Blot Protocol Video!

    Type
    Blog Post
    Published
    March 28, 2023, 1:15 p.m.
    ... Addgenie Meghan and Abi run a Western blot and share tips for this tricky application.    You can check...

  16. Viral Vectors 101 eBook 2nd Edition

    Type
    Blog Post
    Published
    July 11, 2024, 1:15 p.m.
    ...We are very excited to share the latest edition of our Viral Vectors 101 eBook!  This practical guide...

  17. Antibodies 101 Series on TikTok

    Type
    Blog Post
    Published
    March 19, 2024, 1:15 p.m.
    ...isn't your platform, all of our videos will also be shared on our @addgene Instagram account, or you can watch...

  18. 2022 Depositor Week

    Type
    Blog Post
    Published
    Dec. 12, 2022, 2:15 p.m.
    ...revamped Data Hub! The community needs more data-sharing heroes like you.)  ...

  19. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...With this update, we have added new sections to share and describe some incredible new CRISPR technologies...
Showing: 261 - 280 of 499 results