We narrowed to 859 results for: STI

..., a pooled library has hundreds to millions of distinct plasmids, all of which need to be represented ...The size and composition of your library can drastically impact what conditions it needs to properly amplify...pre-made libraries aren’t quite plug-and-play: you’ll still need to titer and optimize before you can transduce...your research. And, of course, if you have any questions, don’t hesitate to email us at [email protected], this is a useful place to ask experimental questions.  McDade, J. R., Waxmonsky, N. C., Swanson, L.... CRISPR libraries. Protocols from the Broad Institute: A variety of protocols and guides regarding pooled...

...echoed in the other testimonies we gathered. The program definitely caused a positive stir and was recognized...invaluable to me as a bench scientist working at an institution where training opportunities weren’t always available...were connected to that Tweet Up in 2013. These existing connections brought in folks I didn’t yet know....  They liked us. They really liked us.  The testimonials from participants in Binning Singletons at ASM...Heather Hendrickson, Jonathan Jacobs, Mo Kaze, Christina Kellogg, Heather Martinez, Pat Schloss, Tara Smith...

...by enhanced green fluorescent protein (EGFP) reconstitution. (a) Scheme of validation for DSB mediated ...mediated EGFP expression cassette reconstitution. When the target sequence was digested by sgRNA guided Cas9 ...SSA, single strand annealing) took place and reconstituted the EGFP expression cassette. (b) pCAG-EGxxFP...details on this plasmid, see Mashiko et al., 2013). Testing the efficacy of your gRNAs is then a simple matter...of gene modification in vivo. Find Plasmids for Testing Your gRNAs in vitro at Addgene CRISPR/Cas9 gene...require you to house mice that may have no mutation, costing money and space. As a rule, screen all of your ...

...promote cell growth. Overgrown cultures stimulate quiescence, the resting phase of the cell cycle; quiescent...lentiviral infection, happy virus is the second. When harvesting lentivirus, some labs prefer to collect and pool...the positively charged polycation, polybrene. Interestingly, one study found that alternative polycations...routinely produce lentivirus may want to consider testing a variety of cation and serum sources to ensure... the best conditions for specific applications, stick with them! Changes in such variables as cell density...

...Leong Chew is a Junior Principal Investigator at the Genome Institute of Singapore. His lab focuses on...with Wei Leong Chew, a researcher at the Genome Institute of Singapore who recently started his own lab....work to an independent position at the Genome Institute of Singapore. Chapter 3: Biology research and ... Singapore. Chapter 4: Life as a new primary investigator. Coming up with new project ideas, applying ...

...multiple streams for composting and recycling in the event the object in question cannot be washed or ...reuse, reducing the need for the production of new plastic.  Recycling and reuse is not limited to the office... however. We participate in many styrofoam and plastic recycling programs in the lab as well. For a more...Practices to the Lab.”  Whether participating in an existing process or proposing new programs, we're always...

...mutations, we still weren’t entirely happy with our editing outcomes and in many cases still didn’t get ...must undergo homology-directed repair (HDR), a distinct cellular DNA repair pathway. Accomplishing this... be screening a total of a few hundred clones - Still better than 10.000, right? (Kwart, Paquet, et al...reads that we studied by deep sequencing, but interestingly (and to our frustration) in many cases we only...currently a Professor for Neurobiology at the Institute for Stroke and Dementia Research, Ludwig-Maximilians-University...

...cells on a flow plot, you might have to do some testing or ask experienced colleagues.   Figure...flow analysis I can spot my lymphocyte population distinct from cellular debris and other larger cells. Note...following gates, cells that are not of interest can still be gated out later. Based on personal preference... many cases a stain will not result in clearly distinct positive and negative populations. Oftentimes,...read an FSC/SSC plot and how to use H vs. A to distinguish single cells from duplicates. Lastly, you learned...Harvard Medical School. His projects include investigating negative regulators of T cells and cytokine ...

... the Institute for Protein Innovation (IPI), a Boston-based, nonprofit protein research institute, partnered...researchers can measure this conformational change to investigate the role of specific integrins in cellular processes...subunit. Using these affinity reagents, the investigators inhibited other RGD-binding integrins, individually...for cell adhesion. The results surprised the investigators, challenging the long-held belief that αVβ3 ...IPI. Caitlin Faulds is a science writer at the Institute for Protein Innovation. She writes about all things...

...advantage for researchers looking to generate long-lasting changes in cells. However, for some applications... genome. Without a working integrase, IDLVs can still deliver their genetic payload to the cell but cannot... IDLVs were first discovered by researchers investigating HIV integrase mutants in the early 1990s (Leavitt...in several patients developing T-cell acute lymphoblastic leukemia due to insertional mutagenesis (Howe...avoid the risk of insertional mutagenesis while still allowing for stable expression of the transgene....of SCID-X1 patients. The Journal of Clinical Investigation, 118(9), 3143–3150. https://doi.org/10.1172/...

...cytosine-to-thymine substitutions in target sequences (Banno et al., 2018). These substitutions were limited ...enable C-to-T and A-to-G base substitutions respectively. Substitutions seem most efficient when using... recombineering took place. This system is distinct from those used in eukaryotes in that CRISPR isn...targeted and preferentially depleted from gut. While still far from the clinic, CRISPR-Cas3 systems have been... Sharp JD, Xu R, Prisic S, Husson RN (2016) Investigating essential gene function inMycobacterium tuberculosisusing...

...Even if we stick to citation counts for a moment, it’s clear that articles in less-prestigious journals ...proportion of highly cited articles appearing in lower-prestige journals has risen steadily from 1995 to 2013.... in citations for older publications. (As an interesting aside, Nature recently reported that the most...of another, perhaps it’s worth reflecting on a question of impact that only you can answer: How would ...

...Feng Zhang (of the Broad Institute of MIT and Harvard and the McGovern Institute for Brain Research) – and...I have an important question: How much coffee does it take to fuel more than 30,000 neuroscientists over...Though, to be fair, I think this is a conservative estimate. As a graduate student, I drank 3-4 cups of coffee...Zhang was awarded one of this year’s SfN Young Investigator Awards and we couldn’t wait to congratulate ...

...portable microscope. Less fluorescence admittedly, but still so beautiful! Enabling the scientist in everyone...buoyancy devices” (also known as gas vesicles) as acoustic biomolecule-based dyes. This technology is widely...about ‘Using ultrasound to image bacteria in vivo: acoustic reporter genes’ on our blog. Creative approaches...telling a story makes scientific topics both interesting and approachable!   The grand finale of visual.... References 1. Bourdeau, Raymond W., et al. "Acoustic reporter genes for noninvasive imaging of microorganisms...

... Human Dynamics Laboratory identified five characteristics of very successful teams. Check out Joanne'...if others are involved. They may feel they are wasting their time. The manager may appear to be too busy...to get people sharing.   Frequently ask direct questions for specific feedback in non-public settings. ...demonstrate patience, pause; A = ask at least one question to clarify the situation; C = confirm that you...employees who volunteer constructive feedback and suggestions. Consider offering rewards (gift card, afternoon...

... suffer any less acutely.  The Rare Genomics Institute works to help those who suffer from rare diseases...Through their BeHEARD Award, the Rare Genomics Institute partners with companies to provide technological...Elia Di Schiavi is a Research Scientist at the Institute of Biosciences and Bioresources in Naples, Italy...of spinal cord motor neurons. This disease is estimated to affect anywhere from 1 in 6,000 to 1 in 19,...prioritize which genes they will use to develop diagnostic tools and animals models of the disease in partnership...

...promoter regulating the gene of interest with a constitutive, robust M24 promoter. Originally isolated from...from the Mirabilis mosaic virus, the constitutively active M24 promoter has been modified with duplicated...this new vector, pSiM24, can also be used with biolistic methods, making it useful for engineering plants...large gene transfer. The M24 promoter provides constitutive, high-level gene expression and is functional...antibiotic resistance gene is controlled by a weaker constitutive Nos promoter, and it can be used to select both...

...in December were developed as an answer to that question. Additionally, a concurrent Nature Biotechnology... really not possible before,” said the Broad Institute's Ophir Shalem. “Whole regions of the genome which...delivery vehicle, Neville Sanjana, also of the Broad Institute, said they were able to produce a very diverse... of one of the Science papers, told the Broad Institute. “This will greatly aid efforts to understand ... RNA Library. Koike-Yusa H, Li Y, Tan E-P, Del Castillo Velasco-Herrera M, Yusa K. Nature Biotechnology...

...was in the lab, if you read a paper and saw an interesting construct you wished to use, you could either...we share? NanoLuc® Luciferase-based tools are fantastic examples of technologies that can accelerate your... fusions for imaging Fluorescent proteins are fantastic imaging tools but their use is limited in vivo... activation of other fluorescent probes but can still transfer energy to the fluorescent protein acceptor...create a calcium sensor with the following characteristics: No photoexcitation High Ion specificity High...Yang, J., et al. (2016) Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing...

...Kaustubh Kishor Jadhav, a Research Assistant at MGMs Institute of Biosciences and Technology. If you are reading...culturist has to deal with this problem. It is estimated that mycoplasma is responsible for up to 60% of...always use a second assay to be 100% sure. Before testing for mycoplasma, the cells should be in continuous... for low levels of contaminations to grow. For testing, the medium should not have been changed for at...regularly and dampness of any kind should be avoided. Distilled water provided to maintain the humidity should...Jadhav, MSc is a Research Assistant at the MGMs Institute of Biosciences and Technology in India. Jadhav...