We narrowed to 351 results for: SUA

1. Antibodies 101: Conventional vs Spectral Flow Cytometry

   Type

   Blog Post

   Published

   Aug. 3, 2023, 1:15 p.m.

   ...fluorescent molecules can be quite broad, detectors will usually only pick up a small portion of that spectra to...

2. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

   Type

   Blog Post

   Published

   June 18, 2020, 1:15 p.m.

   ... After the ELISA step, the narrowed-down pool (usually 10-30) of sybody candidates are sequenced for identification...

3. 3D Printing Meets CRISPR Cas9

   Type

   Blog Post

   Published

   Oct. 17, 2017, 12:55 p.m.

   ...Learn All about CRISPR Use CRISPR for Genome Visualization Tips for a First Time CRISPR User by a First...

4. Fluorescent Tagging of Endogenous Genes with SapTrap

   Type

   Blog Post

   Published

   Oct. 12, 2017, 2:26 p.m.

   ...labor-intensive processes like PCR or by evaluating visual phenotypes. Improving C. elegans fluorescent protein...

5. Fluorescent Proteins 101: Fluorescent Protein Timers

   Type

   Blog Post

   Published

   May 4, 2017, 2:30 p.m.

   ...predominantly fluoresce a second color. This change is usually hypothesized to be a result of chromophore oxidation...

6. Tips for a 1st time AAV user (by a Rookie AAV user)

   Type

   Blog Post

   Published

   Oct. 23, 2018, 11:49 a.m.

   ...enough plasmid to complete my experiment. AAV is usually the limiting reagent for my experiments, so I always...

7. Plasmids 101: 5 factors to help you choose the right cloning method

   Type

   Blog Post

   Published

   Aug. 21, 2018, 12:31 p.m.

   ...happen in a matter of minutes. Cost: Funding is usually limited - what cloning approach should you use ...

8. Tips to Make the Most of a Scientific Conference

   Type

   Blog Post

   Published

   May 23, 2019, 12:26 p.m.

   ...the session, if someone comes up to my poster, I usually greet them and say something like “Let me know ...

9. Plasmids 101: Knockout/Knock-In Plasmids

   Type

   Blog Post

   Published

   Dec. 1, 2016, 3:30 p.m.

   ...gene can affect a cell's phenotype, researchers usually remove it after selection using the Cre/Lox recombination...

10. The Scientific Conference Poster Session: Tips for Success

    Type

    Blog Post

    Published

    Aug. 29, 2019, 12:50 p.m.

    ...engaging with you? Be sure to use your poster as a visual aid, not a crutch. Even the most prepared of us...

11. Antibodies 101: Flow Compensation

    Type

    Blog Post

    Published

    May 16, 2024, 1:15 p.m.

    ...between machines or the program you’re using, but usually you can click on the correct box and either manually...

12. Of Myc and Men

    Type

    Blog Post

    Published

    Jan. 19, 2023, 2:15 p.m.

    ...endoplasmic reticulum localization, Munro and Pelham casually introduced the Myc-tag (Munro, et al. 1986; Munro...

13. PCR Applications for Copy Number Variation Assays

    Type

    Blog Post

    Published

    April 26, 2022, 1:15 p.m.

    ...sequence present in the same amount in all samples (usually to a ubiquitously expressed, one-copy ‘housekeeping...

14. Polymerase Chain Reaction Overview and Applications

    Type

    Blog Post

    Published

    Aug. 10, 2021, 1:15 p.m.

    ...temperature cycling. The final PCR product is often visualized on a diagnostic agarose gel to confirm product...

15. Hot Plasmids and Viral Preps - May 2021

    Type

    Blog Post

    Published

    May 18, 2021, 1:15 p.m.

    ...and more AAV1 versions will be released soon. Visualize axonal projections and putative pre-synaptic terminals...

16. Genome-wide Screening Using CRISPR

    Type

    Blog Post

    Published

    Aug. 18, 2015, 2:30 p.m.

    ...error-prone non-homologous end joining (NHEJ), usually results in a loss-of-function mutation within the...

17. Light Sheet Fluorescence Microscopy

    Type

    Blog Post

    Published

    July 16, 2015, 2:30 p.m.

    ...tissues transparent are enabling microscopes to visualize the cellular architecture of whole tissues in ...

18. Plasmids 101: Multicistronic Vectors

    Type

    Blog Post

    Published

    Sept. 9, 2014, 8:20 p.m.

    ...below. IRES Elements Translation in eukaryotes usually begins at the 5’ cap so that only a single translation...

19. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type

    Blog Post

    Published

    June 12, 2014, 3:30 p.m.

    ... for several different fluorescent proteins. Visualizing fluorescence can be challenging in plants because...

20. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type

    Blog Post

    Published

    Dec. 12, 2013, 3:35 p.m.

    ... the keyboards and displays for control and visualization. Addgene: What do you envision for the future...