... et al, 2008) occasionally and certainly after a set-up has been changed (for instance after replacing...

...gene. In this manner, we have created an initial set of 10 plasmids representing 10 human genes and will...Last updated on Oct 14, 2020 by Seth Kasowitz. This post was contributed by the gene editing team at ...

...coinfect with multiple viruses to express genes or gene sets over 4.7 kb. Co-infection rates for AAVs are generally...

... range of DNA edits.   Cas3 – The shredder What sets Cas3 apart from Cas9 and almost all other Cas enzymes...

...its surroundings and being shaped by them. Another set of highly influential microenvironment cells are ...

...deposit to Addgene. We are also lauded by another set of stakeholders - the tech transfer community. In...

...blast from the past: Cofounder and CSO Melina Fan setting up Addgene's very first in-house NGS run.   ...approvals. In 2013 we were thrilled to receive the Massachusetts Nonprofit Network Excellence Award for our electronic...

...your blot with multiple antibodies. You’ll want to set up your blot with a polyvinylidene difluoride (PVDF...

...activation  and base editing, whereas a different set of mutations (the “V3.1” version) works best for ...

... Karmella Haynes at Emory recently contributed a set of plasmids to the repository that will help epigenetic...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...

...connections This is a site for meeting people. Use the settings to let people see who you are when you look at...

...tracked and quantitatively analyzed by sequencing its set of barcodes. CombiGEM is highly flexible and can ...

... a small subset of cells? Other proteins may also be utliized as scaffolds, including the set of red fluorescent...recombination at LoxP sites. Placing a lox-STOP-lox cassette upstream of a gene of interest blocks transcription...absent. When Cre is present, it removes the STOP cassette, activating gene expression. Although Cre isn’...

...for this transition. Second, a coach will help you set goals and then hold you accountable for carrying ...

...profiles have limited their usability in clinical settings. On the other hand, vectors derived from AAV have...the gene RPE65. It is characterized by childhood onset of blindness and was thought to be untreatable until...

...case, you design your targeting vector such that a set of three LoxP sites flank the resistance gene and...targeted gene. Upon recombination, the GFP/NeoR cassette is inserted in place of exon 2. Thus, the targeted...sequence flanked by LoxP sites while the resistance cassette has been placed into an intron.    Figure...

...Recently Seán got the entrepreneurial bug and has set up an ELISA kit company on whose website you can ...

...especially for systemic approaches, has its own unique set of obstacles. Thus far, several targeted therapies...

...sample, we’ll then load it on to our new MiSeq and set up the run making sure to note the appropriate barcode...

...happens when the antibody is produced from a new set of animals), and wrong application (different experiments...