We narrowed to 630 results for: Son
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TypeBlog PostPublishedMarch 27, 2014, 5:14 p.m....Verkhusha popping up again and again, and for good reason. We all know scientists have used fluorescent proteins... higher resolution images in vivo. “One of the reasons we can’t localize one cell at a depth of one centimeter...
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An Addgene Co-op: The Intersection of Fun, Friendship, and Work
TypeBlog PostPublishedJune 29, 2023, 7 p.m....approached me and asked about my current projects and personal interests. We chatted about social media analytics... being genuinely interested in knowing you as a person, builds a strong sense of trust between coworkers... -
Plasmids 101: Gene, enhancer, and promoter trapping
TypeBlog PostPublishedMay 2, 2023, 1 p.m....monitored genes and mutants - viral integration, transposon, and many more! Check out Addgene’s catalog to...resources on the Addgene blog Plasmids 101: Using Transposons in the Lab Plasmids 101: Screens vs. Selections... -
New Viral Vectors - Fall 2024
TypeBlog PostPublishedOct. 3, 2024, 1:15 p.m.... with multiple serotypes pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] AAV1 Optogenetics Edward Boyden New ...resources! This blog post was written by Rachel Leeson and Brian O'Neill. More resources on the Addgene... -
An Addgene Summer Internship
TypeBlog PostPublishedAug. 8, 2024, 7:22 p.m....Covid gene, a toxin, or an oncogene, for safety reasons, and the insert size should be a max of 500 bp ...biochemistry in college and play the drum set as their personal instrument. They are the oldest sibling in a Guatemalan... -
Year of the Camelid: Antibody Style
TypeBlog PostPublishedMay 9, 2024, 1:15 p.m.... we love camelids too, though not just for the reasons the UN describes. In honor of the Camelid family...Arbabi-Ghahroudi, 2017)! Figure 1: Comparison of a heavy-chain only antibody and single-domain... -
Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development
TypeBlog PostPublishedNov. 1, 2017, 2:04 p.m.... and then 75% of the time I’m kind of the go-to person for my department.” Caitlin does this by acting... Keown, a staff writer at the non-profit Fred Hutchinson Cancer Research Center. And make sure to subscribe... -
Oh, The Places You Can Go: Careers in Science Communication - Product Development
TypeBlog PostPublishedOct. 18, 2017, 1 p.m.... school, and high school. I’m the only science person of the bunch. They don’t really know where I came... you how to design a course, how to design good lesson plans, how to design test questions, and how to... -
The Blue Flame Award: Celebrating Addgene's Most Requested Depositors
TypeBlog PostPublishedNov. 14, 2016, 3:30 p.m....have popular plasmids but lack the resources or personnel to constantly prepare them for distribution. One...for sharing your plasmids at Addgene. You are the reason we’re able to do what we do! Special ... -
Management for Scientists: What Makes a Good Manager Anyway?
TypeBlog PostPublishedDec. 9, 2014, 2:22 p.m....Flexible work schedule Flexibility to manage their personal lives to achieve a healthy work-life balance. ...new workplace if opportunities for learning and personal growth do not exist or if they feel underutilized... -
5 Uncommon Ways to Gain Career Skills During an Uncommon Time
TypeBlog PostPublishedOct. 20, 2020, 1:15 p.m....mentoring groups seem to be as successful as in person groups. Maybe it’s just that it’s easier for everyone...A How-To Guide for Scientists. Once in-person, peer-mentoring groups at Addgene have gone virtual... -
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools
TypeBlog PostPublishedJune 27, 2018, 1:16 p.m....podcast segment Similarly to the Carr lab, Erik Sontheimer and his group from the University of Massachusetts... surrounding the dSpyCas9-APEX2 fusion. This Sontheimer lab used this technology, which they call C-BERST... -
In Vivo Biotinylation of Bacterial Fusion Proteins
TypeBlog PostPublishedJan. 25, 2018, 2:09 p.m....multitude of methods including surface plasmon resonance (Biacore, GE Life Sciences) or bio-layer interferometry...protein on blots with Streptavidin-phosphatase (Jackson ImmunoResearch, 016-050-084; 0.2 μg/ml final). ... -
The Golden Stopwatch Award: Recognizing Facilitators of Research
TypeBlog PostPublishedSept. 5, 2019, 1:06 p.m.... were excited to deliver the very first ones in person by our own Tech Transfer Team! Why MTAs Matter ...Laboratory Dana-Farber Cancer Institute Fred Hutchinson Cancer Research Center Memorial Sloan-Kettering... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog PostPublishedDec. 13, 2018, 1:14 p.m....monoclonal antibody production (Oyaas, 1989; Ozturk and Palsson, 1991; Oh, 1993) and increased yields in adenoviral...272-278. 4. Ozturk, Sadettin S., and Bernhard O. Palsson. "Effect of medium osmolarity on hybridoma growth... -
Intro to Our Summer SciComm Series!
TypeBlog PostPublishedJune 9, 2022, 1:15 p.m....communication (it is, after all, how one moves from layperson to expert) and peers can and do consume science... Rachel Leeson... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog PostPublishedJune 22, 2021, 1:15 p.m....Cele S, Gazy I, Jackson L, Hwa S-H, Tegally H, Lustig G, Giandhari J, Pillay S, Wilkinson E, Naidoo Y, Karim... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog PostPublishedMay 25, 2021, 2:29 p.m....Bradbury ARM, Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson S, Bladen CL, Jones B, Aldred SF, ... -
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC
TypeBlog PostPublishedSept. 21, 2023, 1:15 p.m.... and then applying this mixture to your tissue (Tuson, et al., 1990; Goodpaster, et al., 2013). One issue...983. https://doi.org/10.1177/002215549804600813 Tuson JR, Pascoe EW, Jacob DA (1990) A novel immunohistochemical... -
Summer SciComm: Science Storytelling
TypeBlog PostPublishedSept. 16, 2022, 1:15 p.m....thought about how X works?”, or even by introducing a person that the audience can relate to. It doesn’t need... Rachel Leeson...