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We narrowed to 453 results for: Spr

Showing: 261 - 280 of 453 results

  1. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...efficiently in just a few steps. More recently, CRISPR technology has adapted Golden Gate cloning for ... you are using the Golden Gate method to create CRISPR/Cas9 constructs, assemble standard plasmids parts... Check out Addgene’s restriction cloning guide! CRISPR 101: Multiplex Expression of gRNAs Choosing a Molecular...

  2. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...feature prevents the virus from replicating and spreading in non-target cells, including yourself as the...shields are required whenever there’s a splash or spray risk in BSL-1. In BSL-2 labs, eye protection is ...take extra steps to ensure that particles are not sprayed into the air. For lentivirus and other BSL-2 materials...

  3. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...their hosts to keep them around and even help them spread. They can be inherited by daughter cells during..., similar to the host’s chromosome, or they can spread between hosts through horizontal (or “lateral”)...when encoded on plasmids, they can promote rapid spreading of genetic material through a population of cells...

  4. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...several advantages over the alternative systems – CRISPR, siRNA, etc. Firstly, they are reversible mechanisms...protein for imaging. Given its popularity and widespread usage, a proteolysis-targeting chimera (PROTAC....1073/pnas.1216880109 Resources on Addgene.org CRISPR Plasmids – Protein Tagging CRISPaint Gene Tagging...

  5. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...purposes, like creating stable cell lines, delivering CRISPR/Cas9 to cells for genome editing, and introducing... is gradually reduced. For example, after using CRISPR/Cas9 to edit a gene, the expression of Cas9 is ...High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nature Biotechnology...

  6. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ... Gilbert Labs have previously deposited their CRISPRoff system — a programmable epigenetic editor consisting...deposited with Addgene. These variants, including a “miniCRISPR” version, are much smaller proteins than SpyCas9...Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing. Cell, 184(9), 2503–2519...

  7. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...electrical activity to gene expression, and even CRISPR based gene editing. But, how do you decide which...Photoswitching Proteins (Vol. 2173, pp. 21–51). Springer US. https://doi.org/10.1007/978-1-0716-0755-8_...

  8. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...encodes a Type I-F CRISPR-Cas system. This system has four major components: 1) a CRISPR RNA (crRNA), 2) ...sets the stage for combining the super powers of CRISPR and transposons to allow for the targeted delivery...

  9. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ... first ever CRISPR-based clinical trial in 18 lung cancer patients. This study used CRISPR gene editing...create welcoming environments for tumor cells spreading to other organs during metastasis. Evading Recognition...

  10. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    Published
    Nov. 26, 2024, 2:15 p.m.
    ...about recent CRISPR deposits from Arlene Sharpe’s lab. X-Chime and Performing In Vivo CRISPR Screens Using...

  11. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    Published
    May 17, 2017, 3:37 p.m.
    ...plasmids covered in this segment Hot Plasmid 1: CRISPR X from Michael Bassik's Lab - A new tool for making...localized point mutations. Listen to Our Segment on CRISPR X Hot Plasmid 2: Magneto 2.0 form Ali Guler's Lab...

  12. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    Published
    Nov. 13, 2015, 3:30 p.m.
    ...Architect Pam McA' Nulty (check out our blog post on CRISPR software to see the importance of programming for...Scientist Joel McDade’s presentation on the emerging CRISPR genome editing technology was a rousing success...

  13. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...Resources on the Addgene Blog Read Our Post on CRISPR Methods for Bacterial Genome Engineering Learn ...Addgene.org Check out Our Microbiology Resource Page CRISPR plasmids designed for use in bacteria Deposit Your...

  14. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    Published
    Jan. 2, 2014, 3:44 p.m.
    ...you’re looking for. Tip 3: Share protocols and spreadsheets with lab members Now that you’ve organized your... may also want to consider moving your paper spreadsheets for reagent storage locations, such as the list...

  15. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    Published
    July 20, 2017, 2:30 p.m.
    ...proteins to monitor the localization of these genes, CRISPR to disrupt their function, and C. elegans overexpression...peritoneal mesothelioma tumorigenesis. They plan to use CRISPR and lentiviral plasmids from Addgene to knockout...

  16. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ..., Flp, KD, R, B2 and B3, approximately 50% of offspring carried both the recombinase and GAL4, indicating...normally and thus nontoxic. In contrast, 0/79 offspring carried both Cre and GAL4, further emphasizing...

  17. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ...social media data, and I was given a fantastic spreadsheet template to build out a report on. I was able...upper management and building out social media spreadsheets every day of my co-op. I had to do research ...

  18. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ..., they eliminate the expression of the gene (by CRISPR knockout, shRNA depletion, etc.) and ask what the...e67667. DOI: https://doi.org/10.7554/eLife.67667 Springer, S. P. Gene Traps. Plant Cell. (2000) 12(7): 1007...

  19. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...with the Addgenies on March 11, 2020 after rumors spread in our building that someone from a different company...enough funds to make most of our payroll for the spring and early summer months. We are also extremely ...
Showing: 261 - 280 of 453 results