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We narrowed to 542 results for: TIL

Showing: 261 - 280 of 542 results

  1. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...research in a lab in California. I felt then, and still feel now, that it is important to take advantage...often don’t tell you what company a job is with until just before the interview). This wasn’t exactly ...

  2. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ... directly injecting DNA into the pronuclei of fertilized eggs, which leads to random incorporation of ...genome after injection into the cytoplasm of a fertilized egg, a process which is inefficient when DNA ...

  3. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...’ve found that the advice in these resources is still valuable for anyone in the lab though, including...but didn’t realize I was interested in microbes until the end of my second year! Conclusion and other...

  4. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...and circularization of dsDNA (6). Less commonly utilized in vitro, Taq DNA ligase will ligate only nicks...Despite this higher fidelity, Taq DNA Ligase can still detectably ligate many T:G, T:T, and A:C mismatches...

  5. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...cells in the population, so some of the cells will still appear green despite having active Cas9 (see tip...site in the middle of the PCR product, you would still be able to identify the cleavage product. 3. The...

  6. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...Twitter.   References Arribere JA, Bell RT, Fu BXH, Artiles KL, Hartman PS, Fire AZ (2014) Efficient Marker-Free...Schmidt H, Krause MW, Seydoux G (2014) Scalable and Versatile Genome Editing Using Linear DNAs with Microhomology...

  8. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...Sheet Microscopy Light Sheet Microscopy typically utilizes a configuration of two or more objectives to create...monolayers of live cells with fluorescently labelled, motile structures These samples are thin enough to sit...

  10. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ... cancer, multiple immunotherapy targets can be utilized at the same time. A combination of tolerance marker...the development of immunotherapeutics. There are still many important questions in this exciting field ...

  11. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...we call CRISPR, by any other name would…probably still edit genomes. Understanding the different types... in archaea, though Class 1 remains largely underutilized compared to Class 2. Class 1 Despite their incredible...

  12. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...control but will not make the plasmids available until: you notify us with publication details or permission...that will make it easier for scientists to best utilize your plasmids (these can be protocols, GenBank ...

  13. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...armed with the highest-competency cells you will still struggle to get transformants if the plasmid you...buffers used to ensure proper lysis, but you will still face column capacity limitations since the columns...

  14. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...CGBEs. What's next? Base transversion editing is still advancing rapidly. If it follows a similar path ... synthesized, templated sequence. And the story still isn’t over! Keep an eye out for our next post about...

  17. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...sample when developing the protocol and optimized until the multiplexed and individual values match across... isn’t the end of your analysis process. You’ll still need to do statistical analysis to determine if ...

  18. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...). Efficient retrograde neuronal transduction utilizing self-complementary AAV1. Mol Ther 16, 296–301....Axonal Transport: Cargo-Specific Mechanisms of Motility and Regulation. Neuron 84(2), 292–309. PubMed ...

  19. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ... contain any denaturing agents. However, you’ll still have to lyse your proteins. For this post, we are...systems are fast (often <10 minutes) and simple, utilizing individually packaged membrane ‘sandwiches’. They...

  20. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...These are only starting recommendations; you will still need to experimentally determine the best titer ...efficiency across species points to its potential utility in translational applications and in models such...
Showing: 261 - 280 of 542 results