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...and therefore it is important that the digest go until completion. The time required for complete digestion...colonies are a result of uncut empty plasmid, you will still have colonies when you do not add ligase. If the...

...research in a lab in California. I felt then, and still feel now, that it is important to take advantage...often don’t tell you what company a job is with until just before the interview). This wasn’t exactly ...

...and circularization of dsDNA (6). Less commonly utilized in vitro, Taq DNA ligase will ligate only nicks...Despite this higher fidelity, Taq DNA Ligase can still detectably ligate many T:G, T:T, and A:C mismatches...

...cells in the population, so some of the cells will still appear green despite having active Cas9 (see tip...site in the middle of the PCR product, you would still be able to identify the cleavage product. 3. The...

...Twitter.   References Arribere JA, Bell RT, Fu BXH, Artiles KL, Hartman PS, Fire AZ (2014) Efficient Marker-Free...Schmidt H, Krause MW, Seydoux G (2014) Scalable and Versatile Genome Editing Using Linear DNAs with Microhomology...

...invisible to naked eyes and hence cannot be detected until it leads to contamination. Another source of the...regularly and dampness of any kind should be avoided. Distilled water provided to maintain the humidity should...

... directly injecting DNA into the pronuclei of fertilized eggs, which leads to random incorporation of ...genome after injection into the cytoplasm of a fertilized egg, a process which is inefficient when DNA ...

...’ve found that the advice in these resources is still valuable for anyone in the lab though, including...but didn’t realize I was interested in microbes until the end of my second year! Conclusion and other...

...Sheet Microscopy Light Sheet Microscopy typically utilizes a configuration of two or more objectives to create...monolayers of live cells with fluorescently labelled, motile structures These samples are thin enough to sit...

...immune response, but the study of these dynamics was still laborious as epifluorescence microscopy wasn’t able... provide quantitative measurements of cellular motility, interactions, and response dynamics. In the past...

... cancer, multiple immunotherapy targets can be utilized at the same time. A combination of tolerance marker...the development of immunotherapeutics. There are still many important questions in this exciting field ...

...we call CRISPR, by any other name would…probably still edit genomes. Understanding the different types... in archaea, though Class 1 remains largely underutilized compared to Class 2. Class 1 Despite their incredible...

...transdifferentiated successfully, limiting the utility of this process. Moreover, it is much easier to...between similar lineages, reprogramming is more versatile and universal.  Many thanks to our guest blogger...

...control but will not make the plasmids available until: you notify us with publication details or permission...that will make it easier for scientists to best utilize your plasmids (these can be protocols, GenBank ...

...armed with the highest-competency cells you will still struggle to get transformants if the plasmid you...buffers used to ensure proper lysis, but you will still face column capacity limitations since the columns...

...CGBEs. What's next? Base transversion editing is still advancing rapidly. If it follows a similar path ... synthesized, templated sequence. And the story still isn’t over! Keep an eye out for our next post about...

...would give everyone with a Ph.D. a green card, but until we get elected to Congress, we are tasked with working...Proactivity is very important in this case.  You can still assist your PI in the peer review process, but remember...

...sample when developing the protocol and optimized until the multiplexed and individual values match across... isn’t the end of your analysis process. You’ll still need to do statistical analysis to determine if ...

...). Efficient retrograde neuronal transduction utilizing self-complementary AAV1. Mol Ther 16, 296–301....Axonal Transport: Cargo-Specific Mechanisms of Motility and Regulation. Neuron 84(2), 292–309. PubMed ...

... contain any denaturing agents. However, you’ll still have to lyse your proteins. For this post, we are...systems are fast (often <10 minutes) and simple, utilizing individually packaged membrane ‘sandwiches’. They...