...resistance marker from the vector (abR, Figure 1). The resolution step (“double crossover”) which leads to the ... program) (Latynski and Valentovich 2014). We routinely clone the purified amplicons upstream and downstream... in 5 days. Counter-selection with sacB has revolutionized allelic exchange. Even now, only half our lab...

...fluorescent aptamer, was identified through directed evolution. First, a pool of RNAs that activate the fluorescence...fluorescence of DFHBI were identified using systematic evolution of ligands by exponential enrichment (SELEX). ... by fluorescence-based selection and directed evolution." Journal of the American Chemical Society 136.46...

...activity of the original, generated using directed evolution (Tei et al., 2023).  PLD can hydrolyze phosphatidylcholine...matter. Plus, for folks interested in directed-evolution methods, the activity-based selection in mammalian...Baskin, J. M. (2023). Activity-based directed evolution of a membrane editor in mammalian cells. Nature...

...external forces such as ionizing radiation, chemotherapeutics, and ultraviolet light. In this post, we will...via cleavage in one of two ways - each junction resolution could happen on the crossing strand (horizontally...repair and genome engineering CRISPR-Cas9 has revolutionized the genome engineering world and made targeted...

...can be used to introduce specific nucleotide substitutions (or deletions) in a tailored manner. The approach...perform alanine scanning mutagenesis or targeted substitution of key residues), and in SNP analysis (to introduce... bases at the 3’-end interfere with plasmid reconstitution, and consequently Taq polymerases cannot be...

...much to learn about their myriad functions and therapeutic potential. Our partners at the Institute for ...proteins. Lucky for us, we now have a two-for-one solution: TurboCas!TurboCas provides the genomic targeting...-type-specific expression has been a promising solution. Recently, the labs of Gord Fishell and colleagues...

...and accessibility, more researchers have been utilizing full plasmid sequencing services to verify the...independently increase the frequency of base substitution in human cells. J Biol Chem. 2011 Mar 25;286...E, Fournes F, Cornet F, Hallet B, Rousseau P. Resolution of Multimeric Forms of Circular Plasmids and ...

... transcription factors, or when the gene is constitutively-active or a dominant-negative mutant. Reducing...piggyBac™ transposase. This can be best achieved by utilizing a piggyBac™ transposon in a donor plasmid carrying...and M. H. Wilson, “piggyBac-ing models and new therapeutic strategies.,” Trends Biotechnol., vol. 33, no...Resources on the Addgene Blog Learn about the Sleeping Beauty Transposase Read up on Genome Engineering with ...

...involved in the science, experimental design, and execution. Can lead to an independent project. Lab technician...Other roles you might see Lab manager: managerial duties ex: order materials, lab maintenance Administrative...Administrative assistant: assists PI with admin duties Staff scientist: similar role to a postdoc but more permanent...

...play significantly into the realm of clinical therapeutic development as CRISPR is used not only to study... Dillard, and Ayokunmi Ajetunmobi for their contributions to this work. Søren Hough is a Biochemistry ...PMC4320661. 14. Crosetto, Nicola, et al. "Nucleotide-resolution DNA double-strand break mapping by next-generation...

...cloning rings or following labor-intensive limiting dilution protocols? If this sounds like you, then flow ...control, the higher the level of expression. A routine flow cytometry experiment uses 2 or 3 different...you would need a way to determine the relative contribution of yellow and orange from FITC and that from...

...carried over from the precipitation stage, and then eluting the DNA from the substrate. In a column-based approach... magnets to isolate the beads for the wash and elution steps.  Both bead- and column-based DNA purification...is a large consideration for most labs. Many institutions have preferred vendors which may reduce costs...

...co-founder and CSO Melina Fan's words of welcome! Executive Director Chonnettia Jones and Board Member Joanna...main purpose of the party was to celebrate the contributions of each and every Addgenie, from our co-ops ...

...present our own data on plasmid deposits and distirbution for these fast moving fields.  Addgene is a ...with data comparing Addgene global deposit and distribution trends for Zinc Finger, TALE, CRISPR, and SynBio...

... and Olsson, 1971), plant lectins (wheat germ agglutinin WGA, Schwab et al., 1978) or fluorescent molecules...). Efficient retrograde neuronal transduction utilizing self-complementary AAV1. Mol Ther 16, 296–301....Javoy-Agid F., and Agid Y. (1978). Labeled wheat germ agglutinin (WGA) as a new, highly sensitive retrograde tracer...

...contains 139,868 reagents from 5,821 labs in 1,249 institutions across 55 countries.  Our first request was ...2,002,539 materials to 254,034 scientists in 8,900 institutions across 111 countries. Just eight days into 2024...

...genus members to the specified international institution. Requesters will need to provide a brief description... Addgene will then apply for the license for distribution to the requester’s organization. A non-refundable...

...Investigator III at Novartis, a multinational pharmaceutical company based in Switzerland. After completing...department at MIT. Crystal now works on protein therapeutics at Novartis - listen to learn more about Crystal...

...now with more than 50% international plasmid distribution!) Chapter 8 - Opportunities to create repositories...Genetics Institute in Cambridge, MA and Sequana Therapeutics in San Diego, CA. She earned a B.S in Biology...

...high risk by these screens can then undergo more routine screening allowing for early cervical cancer detection... our Cancer Pages Learn about Global Plasmid Distribution ...