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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 631 results for: UTY

Showing: 261 - 280 of 631 results

  1. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ... Variation in ori structure allows plasmids to utilize diverse host replication machinery. For instance...Paracoccous denitrificans, Pseudomonas fluorescens, P. putida, Rhizobium meliloti, R. leguminosarum by. viciae... P. C., & Wong, H. L. (2023). Development of constitutive and IPTG-inducible integron promoter-based expression...

  2. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ... variety of fields in order to provide useful solutions to complex challenges. As a cross-disciplinary...citation rates to measure the impact of their contribution. It offers a 5-step process and several examples...Social Networking for Scientists Read about the Evolution of Scientific Impact Data Freedom: The Expansion...

  3. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...; multiple codons encode single amino acids. Evolutionary constraints have molded which codons are used...express well in any organism. So what are the evolutionary constraints that lead to these preferences and...6. Pechmann, Sebastian, and Judith Frydman. "Evolutionary conservation of codon optimality reveals hidden...

  4. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...in vivo experiments and in biotechnology and therapeutics (Mauro and Chappell 2014).  Ultimately, it is...critical analysis of codon optimization in human therapeutics. Trends in Molecular Medicine 20:604–613  https...ZE, Kimchi-Sarfaty C (2011) Understanding the contribution of synonymous mutations to human disease. Nat...

  5. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...resistance marker from the vector (abR, Figure 1). The resolution step (“double crossover”) which leads to the ... program) (Latynski and Valentovich 2014). We routinely clone the purified amplicons upstream and downstream... in 5 days. Counter-selection with sacB has revolutionized allelic exchange. Even now, only half our lab...

  6. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...fluorescent aptamer, was identified through directed evolution. First, a pool of RNAs that activate the fluorescence...fluorescence of DFHBI were identified using systematic evolution of ligands by exponential enrichment (SELEX). ... by fluorescence-based selection and directed evolution." Journal of the American Chemical Society 136.46...

  7. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...activity of the original, generated using directed evolution (Tei et al., 2023).  PLD can hydrolyze phosphatidylcholine...matter. Plus, for folks interested in directed-evolution methods, the activity-based selection in mammalian...Baskin, J. M. (2023). Activity-based directed evolution of a membrane editor in mammalian cells. Nature...

  8. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...can be used to introduce specific nucleotide substitutions (or deletions) in a tailored manner. The approach...perform alanine scanning mutagenesis or targeted substitution of key residues), and in SNP analysis (to introduce... bases at the 3’-end interfere with plasmid reconstitution, and consequently Taq polymerases cannot be...

  9. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...external forces such as ionizing radiation, chemotherapeutics, and ultraviolet light. In this post, we will...via cleavage in one of two ways - each junction resolution could happen on the crossing strand (horizontally...repair and genome engineering CRISPR-Cas9 has revolutionized the genome engineering world and made targeted...

  10. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ...be dominant-negative and/or toxic. A potential solution is to include an in-frame degron, or degradation...in a single step and serve as donors for two consecutive CRISPR-mediated HDR events which results in the...Gibbs D, Kim SR, Maguire A, Rex TS, Di Vicino U, Cutillo L, Sparrow JR, Williams DS, Bennett J, Auricchio...

  11. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...much to learn about their myriad functions and therapeutic potential. Our partners at the Institute for ...proteins. Lucky for us, we now have a two-for-one solution: TurboCas!TurboCas provides the genomic targeting...-type-specific expression has been a promising solution. Recently, the labs of Gord Fishell and colleagues...

  12. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ...to Addgenies Brian O’Neill and Ina Ersing for contributing their expertise! This post was originally published...efficiency in pancreatic beta and alpha cells utilizing various AAV serotypes. Scientific Reports, 15(... AAV5 Both Require Sialic Acid Binding for Hemagglutination and Efficient Transduction but Differ in Sialic...

  13. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...and accessibility, more researchers have been utilizing full plasmid sequencing services to verify the...independently increase the frequency of base substitution in human cells. J Biol Chem. 2011 Mar 25;286...E, Fournes F, Cornet F, Hallet B, Rousseau P. Resolution of Multimeric Forms of Circular Plasmids and ...

  14. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ... transcription factors, or when the gene is constitutively-active or a dominant-negative mutant. Reducing...piggyBac™ transposase. This can be best achieved by utilizing a piggyBac™ transposon in a donor plasmid carrying...and M. H. Wilson, “piggyBac-ing models and new therapeutic strategies.,” Trends Biotechnol., vol. 33, no...Resources on the Addgene Blog Learn about the Sleeping Beauty Transposase Read up on Genome Engineering with ...

  15. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...involved in the science, experimental design, and execution. Can lead to an independent project. Lab technician...Other roles you might see Lab manager: managerial duties ex: order materials, lab maintenance Administrative...Administrative assistant: assists PI with admin duties Staff scientist: similar role to a postdoc but more permanent...

  16. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...play significantly into the realm of clinical therapeutic development as CRISPR is used not only to study... Dillard, and Ayokunmi Ajetunmobi for their contributions to this work. Søren Hough is a Biochemistry ...PMC4320661. 14. Crosetto, Nicola, et al. "Nucleotide-resolution DNA double-strand break mapping by next-generation...

  17. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...cloning rings or following labor-intensive limiting dilution protocols? If this sounds like you, then flow ...control, the higher the level of expression. A routine flow cytometry experiment uses 2 or 3 different...you would need a way to determine the relative contribution of yellow and orange from FITC and that from...

  18. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...carried over from the precipitation stage, and then eluting the DNA from the substrate. In a column-based approach... magnets to isolate the beads for the wash and elution steps.  Both bead- and column-based DNA purification...is a large consideration for most labs. Many institutions have preferred vendors which may reduce costs...

  19. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...lab recommends using the Epicentre QuickExtract solution to perform this step to extract genomic DNA from... Zhang lab uses around 50 µl of the extraction solution, runs the protocol, then uses around 2–4 µl in...Mukherjee, K., & Carroll, D. (2013). Donor DNA Utilization During Gene Targeting with Zinc-Finger Nucleases...

  20. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...co-founder and CSO Melina Fan's words of welcome! Executive Director Chonnettia Jones and Board Member Joanna...main purpose of the party was to celebrate the contributions of each and every Addgenie, from our co-ops ...
Showing: 261 - 280 of 631 results