We narrowed to 460 results for: abo.3

...Kinases: they regulate many proteins, with ~1/3 of human proteins predicted to be phosphorylated on at...Blog Learn about photoswitchable kinases Learn more about fluorescent biosensors Learn about using phosphoserine...

... guest Drew Endy, in celebration of open science. 3)      Also in June, the G8 Science Ministers issued...study probably doesn’t say as much about open access as it does about the state of peer review. 8)     ...University Open Data Access (YODA) Project, “a collaborative endeavor guided by aspirations to promote open...

...to allow for antibodies to enter the cell (Figure 3). A useful side effect of the fixing/permeabilization...cytosolic procedure in that case.     Figure 3: For intracellular labeling cells need to be fixed..., you may want to look at other methods. In the above cases, a good alternative is using a two-protein... region of the primary antibody (Figure 1b). As above, the advantage of this system is increased flexibility...

... it from carrying out its proper function (Figure 3).  The Hehnly Lab used such a strategy to dissect ...injection of plasmids and other materials.    Figure 3: Optogenetic inhibition of Rab protein vesicle trafficking... Check out our Optogenetics Guide to learn more about how these modules work, and read on to learn how...to the cell membrane isn’t powerful enough, what about altering the chemical composition of the membrane...

... et al. designed gRNAs to target the HIV-1 5’ and 3’ LTRs and expressed them along with Cas9 in the T-...eradicating HIV-1/AIDS. J Neurovirol. 2015 Jun;21(3):310-21. PubMed PMID: 25716921. Additional Resources...reservoirs, chronic inflammation, and negative metabolic effects of ART. Why can’t the body mount an immune...

...Blog (other programs are discussed below). Chpater 3: SITN Popularity and Events - Amy discusses some of...To learn more about the STIN's podcast, email [email protected]    For more info about starting your...evens where scientists can grab a beer and chat about their work at a local bar. It doesn't have to be...1: Introduction - Vini and Amy tell us a little about themselves. Chapter 2: What is Science in the News...science communicators. In this chapter we learn about some of their events - science seminars, science...the lab and working with SITN. She points to collaboration and delegating as keys achieving success. Chapter...your own SciComm organization and to learn about some of SITN's other initiatives, email [email protected]...

...for making scientific graphics. PS: they’re free. 3. Fluorescent Proteins 101: GFP Fusion Proteins - Making...mNeonGreen, mTFP1, and mWasabi by Jennifer Tsang Learn about mNeonGreen, mTFP1, and mWasabi and find plasmids...Broccoli, Spinach, and Corn, for example. Learn more about the aptamer soup in this blog post. 5. Five Popular...

...the human kinome. These plasmids contain the same 3-4 gRNAs found in the first subset of the pooled library...numbers that’ll leave you, well, #sad. Learn More About CRISPR Pooled Library Screening Why the kinome?...

... bar to quickly find what you’re looking for. Tip 3: Share protocols and spreadsheets with lab members...experiments before the next R01 is due. But what about organizing yourself and your lab? Organizing your...

...tissue-penetrating wavelength above 600 nm. LumiFluors Schaub, F.X., et al. (3) designed two imaging probes...PMID: 27465542. PubMed Central PMCID: PMC4974478. 3. Schaub, F.X., et al. (2015) Fluorophore-NanoLuc BRET...Blog Learn more about FRET Tips for Using FRET in Your Experiments Learn more about Fluorescent Protein...heme-containing proteins in whole animals, light at or above 600 nm wavelengths should be used. Researchers often...be excited by NanoLuc® luciferase (NLuc) and set about to create an in vivo imaging reporter by linking...

...For treatment, it is best to identify a cocktail of 3-12 phages. Once these phages are identified, they ...open science Find blog posts about scientific sharing Find blog posts about networking Additional resources...

... Transcriptional repression DNA Methyltransferase 3 Alpha (DNMT3A) Vlatka Zoldoš’ lab has deposited pdCas9...available as ready-to-use lentivirus. Figure 3: dCas9-DNMT3A methylates cytosines at promoters and...modifications. Like the TALE-LSD1 system described above, dCas9-LSD1 inactivates targeted enhancers. Although...setting up your experimental system. Let us know about your experience with these constructs in the comments...

... leaving behind any accessory DNA sequences [2], [3]. Simply put, the piggyBac™ transposon containing ...PMID: 26702451. PubMed Central PMCID: PMC4686154. 3. A. M. Singh, D. W. Perry, V. V. A. Steffey, K. Miller...transposase itself, please read on to learn more about this exciting technology from the folks at Transposagen...Additional Resources on the Addgene Blog Learn about the Sleeping Beauty Transposase Read up on Genome...

...variants. Klapoetke et al., Nat Methods 2014 Mar 11;(3):338-46. For more information and descriptions of...counterparts. The Janovjak lab has deposited their 3 Opto-RTK constructs – Opto-mFGFR1, Opto-hEGFR, and...turn green. Mashiko et al., Sci Rep 2013 Nov 27; 3:3355. Rinehart lab reagents for improved expression...96-102. Druckmann et al., Neuron. 2014. Feb 5;81(3):629-40.  Next-Gen Brainbow toolkit for neuronal...2012 Mar 20;109(12):E690-7. For further reading about SpyLigase technology, read the Addgene interview...; DOI: 10.1021/sb4001504. For further reading about Nicola Patron's MoClo kit and her plant synbio research...in optimizing TALEN assembly and activity? The laboratory of Takashi Yamamoto has created a complete TALEN...

...be automatically adjusted every 3 years. In May 2016, Secretary of Labor Thomas Perez updated the overtime...the Addgene Blog How did this change in labor laws come about? In March 2014, the White House issued a...must be above this level, and certain job duty tests must be met. In the Department of Labor’s general... to $47,476 per year, under updates to the Fair Labor Standards Act (FLSA). This is having a major effect...ensuring that either all affected scientists are paid above this threshold or for tracking the hours that these... a memorandum to the Secretary of Labor to update the FLSA, which had not seen revisions since 2004. In... In July 2015, the Department of Labor issued a Notice of Proposed Rulemaking, soliciting feedback by ...

...Tris-acetate 40–500 kDa Tris-tricine 150 V, 1–3 hours Good for higher weight proteins Long running...very large proteins. A semi-dry transfer is faster — 3–30 minutes — and uses less buffer, but it is not as...approaches best suit your experimental needs. Table 3: Optimizing the technical design of a western blot...If you’ve ever run a western blot, or thought about running one, you’ll know there’s a lot of choices...

...cyanobacterium Vampirovibrio chlorellavorus." PeerJ 3 (2015): e968. PubMed PMID: 26038723. PubMed Central... Additional resources on the Addgene blog Read about company culture at Addgene See some of our previous...

...for more on timely submission of a job application. 3) Read job descriptions carefully Be open minded and... your strengths in your resume and don't be shy about asking for help. The worst thing that can happen...

...to wash Eppendorf tubes which were to be used for 3 months before they could be discarded.  Seeding Labs...Reddit AMA  Few of us spend much time thinking about conserving resources in the labs. Disposable plastic...Dudnik, Founder of Seeding Labs, tells the story about working as a student in a rice lab in the Ivory ...didn’t feel the need to reuse plasticware or think about conserving reagents and buffers.  However, I had...environmentally conscious friends who had me thinking about energy conservation even before it was a popular...Turns out, one could make a lifetime supply for about $10 US dollars worth of glass powder pruchased from...So, I designed my own gel box (see illustration above) and bribed the guys in the hospital machine shop...

...it doesn’t help much if the actual response takes 3 days and still doesn’t even answer your question. ...not even get a solution to the problem you called about. Next, you try an email, given that this is the ...” next to an encircled question mark (see image above). This useful widget allows you to access our Help... or you just have a general question or concern about the answers we provide, within the widget you also...Addgene Blog Read other Inside Addgene Posts Learn about New Features on Our Plasmid Pages Resources at ...