Skip to main content

We narrowed to 911 results for: addgene

Showing: 261 - 280 of 911 results
  1. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ...editing LARRYv2 pooled libraries for cell barcoding Addgene’s own AAV6 and AAV11 packaging plasmids Mapping ...planning on lounging poolside or swimming laps, Addgene has pooled libraries for any summer project. While...  New AAV6 and AAV11 packaging plasmids from Addgene By Meghan Rego Attention AAV users: in an effort...effort to expand your gene delivery toolkit, Addgene now offers packaging plasmids for AAV6 and AAV11! Why...projection neurons. Created with BioRender.com. Addgene has tested these capsid plasmids in vitro and uses... viral vectors ready to use in your lab. Find Addgene’s AAV6 and AAV11 packaging plasmids here!   An ...
  2. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...Antibodies Resources on addgene.org Addgene's Antibody Purification Protocol Addgene's Video Antibody Purification...of recombinant antibodies (rAbs) exist (though Addgene has a great collection of ready-to-use rAbs and... are contained in (Randolph et. al., 2018). At Addgene, we ensure the efficacy of our antibodies through...Resources and references More resources on the Addgene blog Introduction to Antibodies Plasmid-based Recombinant...Purification Protocol Addgene's Antibody Guide References Trimmer JS. Recombinant Antibodies in Basic Neuroscience...
  3. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...Resources on Addgene.org Learn how Addgene produces AAV and lentivirus Check out Addgene's virus protocols...needed to expand the HEK293 cells. Check out the Addgene AAV Production protocol for more details. Pro tips...because I don’t have enough AAV. Compared to the Addgene AAV Production protocol, I take a lazier approach... the qPCR run and data analysis. Refer to this Addgene AAV Titration by qPCR Protocol for more details...PubMed PMID: 18265393. Additional resources on the Addgene blog Find out what makes AAV a versatile tool ...
  4. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    Published
    Jan. 23, 2018, 2:04 p.m.
    ...Check out Addgene’s Step-By-Step Protocol Pressed For Time? Order Ready-to-Use AAV from Addgene ...PubMed PMID: 18265393. Additional Resources on the Addgene Blog Important Considerations When Using AAVs ...for Gene Expression in Mammals Resources on Addgene.org Find AAV Packaging and Transfer Plasmids Here...
  5. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ...Follow @Addgene, use the hashtag #DeckTheLab, and tag @Addgene in the post! In January, Addgenies will ...or Instagram with #DeckTheLab and tagging us, @addgene to be judged! In previous years, scientists have...
  6. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

    Type
    Blog Post
    Published
    Nov. 8, 2016, 3:30 p.m.
    ... Sciences in Ghana will be receiving the third Addgene/Seeding Labs plasmid grant and plans to use the...future. Future awardees We will be opening the Addgene/Seeding Labs Plasmid Grant to a third round of ...inspiring research. Additional Resources on the Addgene Blog Learn How We Raised Money for Rare Disease...Grants to Rare Disease Research Resources on Addgene.org Check out our Cancer Pages Learn about Global...
  7. Editor's Choice, July 2016

    Type
    Blog Post
    Published
    Aug. 5, 2016, 3 p.m.
    ... them. July 2016 was a fantastic month for the Addgene blog. We achieved roughly 50,000 views over the... Science Communications Intern (and soon to be Addgene Scientist!) Mary Gearing, this post focuses on ...to read all of our excellent posts from July: Addgene at the ASM Microbe 2016 Conference Generating Mouse...
  8. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

    Type
    Blog Post
    Published
    Sept. 9, 2025, 1:15 p.m.
    ... and have the biggest impact. We should know — Addgene has built its foundation on this idea! And we believe...high specificity and brightness, now available at Addgene!   {% module_block module "widget_58e2d882-2fb6...experimental requirements. Best-in-class collection at Addgene The GTA contains data on thousands of AAV enhancer...enhancer plasmids, which are all available at Addgene. This allows scientists to choose enhancers that may... or target a very specific cell type. However, Addgene has partnered with the AIBS to showcase the “best-in-class...Science. There are also more resources available at Addgene, including the Allen Institute for Cell Science...doi.org/10.1002/bies.202300044 Resources on the Addgene blog Plasmids 101: Gene, enhancer, and promoter...
  9. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...protocols Sep-OTSλ plasmid - Addgene # 68292 C321.ΔA bacterial strain – Addgene #68306 pCRT7 NT TOPO E17TAG...E17TAG GFP plasmid - Addgene #68295 SupD plasmid – Addgene # 68307 For further technical reference and protocols...flavors’ of Sep-OTS plasmids are available through Addgene, but we have worked most extensively worked with... recombinant phosphoproteins’ available on the Addgene website. Many thanks to guest blogger Natalie ...Protoc. doi 10 (2008). Journal. Resources on the Addgene Blog Verify Your Constructs with Colony PCR Read... Tags for Protein Purification Resources on Addgene.org Find Synthetic Biology Plasmids Find Bacterial...
  10. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    Published
    June 30, 2020, 1:15 p.m.
    ...conference format. Additional resources on the Addgene blog Read all of our blog posts about conferences...scientific conferences have followed suit. Two Addgenies, Susanna Bachle and Yijun Zhang, recently attended...conferences (TAGC and ASGCT, respectively) and Addgenie Angela Abitua hosted a virtual Reproducibility...
  11. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...those rare diseases will receive plasmids from Addgene worth a total of $5000.  Lowe Syndrome One of those...the award from the Rare Genomics Institute and Addgene will make a substantial difference in pushing the...iPS) technology and CRISPR-Cas9 constructs from Addgene to gene-edit mutations into XP genes and other ...analyses.  “We believe that with this award from Addgene, we’ll be able to make big progress in elucidating...
  12. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    Published
    June 26, 2018, 1:33 p.m.
    ... who each are being awarded free plasmids form Addgene to accelerate their research. Read on to learn ...how these researchers plan to use plasmids from Addgene to advance their research on diseases of glycosylation...this, Prof. Videira and collaborators will use Addgene plasmids to immortalize CDG patient-derived immune...and other specific gene sequences available from Addgene. Dr. McMurray hopes that by demonstrating GdnHCl...neural stem cells. The lab will use plasmids from Addgene to construct NFIX expression vectors as well as...
  13. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...placed on the Addgene website, it sets off a chain reaction of events throughout all of Addgene - within the... of thousands of plasmids a day, like we do at Addgene, pen and paper will fail you. The benefits of lab...fun too. Kris Shamloo is a Software Engineer at Addgene and is interested in writing software tools to ...krisshamloo.com     Additional Resources on the Addgene Blog Learn about How We Use Barcodes in the Lab...Track Your Experiments Additional Resources on Addgene.org Find Plasmids for Your Research ...
  14. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ...At Addgene, we love GFP, and we’re always excited when depositors find new ways to make this workhorse...proteins. The T-DOGGs and Cre-DOG are available from Addgene, and we’re eager to see how you use them in your...PMC4096992. Find plasmids from this publication at Addgene. 2. Tang, Jonathan C.Y., et al. “Cell type-specific...26258682. Find plasmids from this publication at Addgene. 3. Kirchhofer, A., et al. “Modulation of protein...Biol. 19 (2012), 117–121. PubMed PMID: 22157958. Addgene Resources Cre-lox Resource Guide Fluorescent Protein...
  15. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...available! by: Ashley Waldron The majority of Addgene's more than 50 antibodies target neuroscience-related...protein tags - a quick search for ‘Myc tag’ in the Addgene catalog pulled up over 13,000 plasmids! Our recombinant...love to hear from you!   Fig. 3: Image of Addgene’s western blot for the myc-tagged protein GFP-smFP-myc...Anti-c-Myc [9E10] page for more details. Image credit: Addgene.   Find anti-c-Myc antibody here!   Evan, G...
  16. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...-59259-301-1:99 Resources on Addgene.org Addgene antibodies Addgene’s antibody guide Antibody protocols...having solubility issues!). Need a GST antibody? Addgene has you covered! His His tags, often called polyhistidine...protocols  Resources on the Addgene blog Plasmids 101: Protein tags Antibodies 101: Epitope tags To Each...
  17. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ... More resources from Addgene The AAV Data Hub Addgene's Viral Services Addgene's Viral Protocols...10.1007/978-3-319-07758-1_2 More resources on the Addgene blog Viral Vectors 101: Viral Applications Viral...
  18. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...PMC5517107. Additional resources on the Addgene blog Subscribe to the Addgene blog Find lab tips for your research...to learn about practices I could bring both to Addgene, and share with my peers. In fact, there was so...resources by sharing plasmids Find plasmids at Addgene ... about conservation in the lab Resources on Addgene.org Sign up for our quarterly newsletter Help other...
  19. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

    Type
    Blog Post
    Published
    Oct. 3, 2017, 12:11 p.m.
    ...Internship with Addgene, I didn’t know a lot about scicomm, but had enjoyed writing a few Addgene guest blog...careers here Resources on Addgene.org Learn more about careers at Addgene ...Twitter @HansPacker. Additional Resources on the Addgene Blog Get tips on how to do your own informational...
  20. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...Additional Resources on Addgene.org Brush Up on Your CRISPR Background with Addgene’s CRISPR Guide Page Find...try, as always, the plasmids are available from Addgene. Happy CRISPRing! References Harrington, Lucas...bioRxiv (2017): 138867. Additional Resources on the Addgene Blog Read about CRISPR Methods for Bacterial Genome... Find CRISPR plasmids at Addgene ...
Showing: 261 - 280 of 911 results