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...sequence of events plays itself out: The researcher breaks eye-contact with any other human in the room. Their...

...binding the protospacer. The resulting double strand break (DSB) stimulates DNA repair mechanisms that can ...

.... However, if the molecules are confined to a subregion of the cell and/or they cannot tumble in 720°,...

...to get recharged. The fun change of pace helped break up the negativity and allowed me to focus on the...

...-type Cas9 and a gRNA generates a double-strand break in the target DNA, which, when repaired through ...

... the human ubiquitin C promoter. Anatomical abbreviations: Gr= Gracile tract, Cu= Cuneate tract, dCST=...

...small victory in a long road to your scientific breakthrough. More Reading: How to manage scientific data...

...CFP), and yellow FP (YFP) were derived. For a breakdown of GFP, it's variants, and their relevant mutations...

...tools in molecular biology. “Retroviruses” is an umbrella term which encompasses 1.) the gamma-retroviruses...

...SRM), a family of imaging techniques developed to break through the resolution barrier while still utilizing...

...and the quality of a standard curve can make or break an experiment. Here, we will provide an overview...

...method for you! And even if you’re not ready to break-up with your DNA purification kit, you can still...

...employer. Your heart is pounding, you’re short of breath, and you’re a little jumpy. You are at another ...

...Zhen, et al. "Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes...

.... Jean Bennett (UPenn) that resulted in the groundbreaking 2001 study showing that AAV-mediated delivery...

...of ~1.8 and an A260/230 of ~2.0. However, let’s break down how different contaminants impact these ratios...

...is sufficient. Controls: Controls can make or break an experiment. The best controls for a colony PCR...

...Science. 1986; 231:699-704. PubMed PMID: 3080805. 3. Brent, R., and Ptashne, M. A eukaryotic transcriptional...

...facility). The third tube is used to fulfill orders. Breaking the (bar)code At each step in the receiving, transforming...

...studies, act as a reporter for DNA double strand break repair, and to label pathogenic intracellular microbes...