We narrowed to 372 results for: ena

1. Lighting Up Cell Signaling with Photoswitchable Kinases

   Type

   Blog Post

   Published

   July 25, 2017, 1:20 p.m.

   ...the cells with a PP1/PP2A inhibitor leads to maintenance of phosphorylated MEK1/2 levels, suggesting PP1...

2. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

   Type

   Blog Post

   Published

   June 15, 2017, 2:30 p.m.

   ...techniques. Gel filtration and SDS-PAGE under non-denaturing conditions separate proteins based on size. Whether...

3. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

   Type

   Blog Post

   Published

   Dec. 8, 2016, 3:30 p.m.

   ...ccd operon, which is responsible for plasmid maintenance during cell division. ccdB codes for the toxic...

4. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

   Type

   Blog Post

   Published

   Dec. 16, 2015, 3:30 p.m.

   ...specificity, eSpCas9, SpCas9-HF1, and HypaCas9 should enable researchers to make precise edits in mammalian ...

5. The Future of Research Symposium Boston 2015

   Type

   Blog Post

   Published

   Oct. 20, 2015, 2:30 p.m.

   ... of the biomedical research sector, and what a tenable workforce structure may look like. The panel discussion...

6. Plasmids 101: Golden Gate Cloning

   Type

   Blog Post

   Published

   Aug. 27, 2015, 2:30 p.m.

   ...multiple internal restriction sites that may not be amenable to "domestication", you might want to consider...

7. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

   Type

   Blog Post

   Published

   May 5, 2015, 3:54 p.m.

   ...where I dump all of my papers and they all have filenames (year first author name). It’s rudimentary, but...

8. Working with Nuclear Receptors

   Type

   Blog Post

   Published

   July 15, 2014, 4:03 p.m.

   ...of all organisms depends on efficient energy maintenance - through acquisition, storage, and utilization...

9. Hot Plasmids and Viral Preps - March 2021

   Type

   Blog Post

   Published

   March 23, 2021, 1:15 p.m.

   ... is a new system from Jiazhang Lian’s lab that enables simultaneous gene activation, repression, and editing...

10. Hot Plasmids - October 2020

    Type

    Blog Post

    Published

    Oct. 13, 2020, 1:15 p.m.

    ...al., 2020. The researchers tested several scenarios from embryo development to myeloid proliferation...

11. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type

    Blog Post

    Published

    Oct. 6, 2020, 1:15 p.m.

    ...Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H,...

12. How to Prepare for an Industry Interview

    Type

    Blog Post

    Published

    July 9, 2020, 1:15 p.m.

    ... get ready for this interview? Maybe the above scenario sounds familiar to you. It was my experience not...

13. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type

    Blog Post

    Published

    May 7, 2020, 1:15 p.m.

    ...to use non-standard Cas9 in the ABE8 complexes enables a broader targeting scope for base editing.  Try...

14. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type

    Blog Post

    Published

    Aug. 20, 2019, 12:31 p.m.

    ...studies. References Stoica L, Ahmed SS, Gao G, Sena-Esteves M. Gene transfer to the CNS using recombinant...

15. Open Resources and Plasmid Tools For Studying C. elegans

    Type

    Blog Post

    Published

    July 18, 2019, 12:55 p.m.

    ... "The auxin-inducible degradation (AID) system enables versatile conditional protein depletion in C. elegans...

16. Viral Vectors 101: Calcium Sensors

    Type

    Blog Post

    Published

    Aug. 10, 2023, 1 p.m.

    ...you can start by asking ‘What is the biological phenomena I want to observe?’ This information, along with...

17. High Complexity Golden Gate Assembly with NEB’s DAD

    Type

    Blog Post

    Published

    Aug. 1, 2023, 1:15 p.m.

    ... K., Cantor, E. J., & Lohman, G. J. S. (2020). Enabling one-pot Golden Gate assemblies of unprecedented...

18. Making CRISPR Plasmids Using Fragmid

    Type

    Blog Post

    Published

    Aug. 29, 2024, 1:15 p.m.

    ... Doench, J. G. (2024). Modular vector assembly enables rapid assessment of emerging CRISPR technologies...

19. Antibodies 101: Stripping and Reprobing Western Blots

    Type

    Blog Post

    Published

    Aug. 1, 2024, 1:15 p.m.

    ... stripping solution uses heat and detergent to denature and remove antibodies but is also likely to remove...

20. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type

    Blog Post

    Published

    May 22, 2018, 1:38 p.m.

    ...deletion). Find the Plasmids Here Plasmid features enable multiple student experiments We used TOPO cloning...