We narrowed to 322 results for: nans

1. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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   Blog Post

   Published

   July 13, 2017, 2:30 p.m.

   ...feature of balancer chromosomes is that they produce dominant phenotypes visible on the flies. For example, ...

2. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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   Blog Post

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   June 22, 2017, 2:30 p.m.

   ...between two molecules more closely is Förster Resonance Energy Transfer, FRET. Additional opportunities...

3. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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   Blog Post

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   Feb. 4, 2020, 2:15 p.m.

   ...fluorescence lifetime imaging microscopy-based Förster resonance energy transfer (FLIM-FRET) in living cells (Figure...

4. Truncated gRNAs for Regulating Gene Expression

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   Blog Post

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   Jan. 10, 2017, 3:37 p.m.

   ...where her research is focused on engineering nanoparticles as drug delivery systems. She is also a NASA...

5. Plasmids 101: Knockout/Knock-In Plasmids

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   Blog Post

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   Dec. 1, 2016, 3:30 p.m.

   ...marker HSV-tk is used to select against random recombinants.   Design your targeting construct. For...

6. Hot Plasmids: Summer 2025

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   Blog Post

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   Aug. 28, 2025, 1:15 p.m.

   ... Find the LARRYv2 Libraries here! Singh, I., Fernandez-Perez, D., Sanchez, P. S., & Rodriguez-Fraticelli...

7. Production of Virus in Insect Versus Mammalian Cells

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   Blog Post

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   Nov. 5, 2024, 8:32 p.m.

   ...). HEK293 cell line as a platform to produce recombinant proteins and viral vectors. Frontiers in Bioengineering...

8. Pooled Library Amplifications

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   Blog Post

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   May 30, 2024, 1:30 p.m.

   ...For larger libraries that require hundreds of nanograms of DNA, you will likely need to perform several...

9. Antibodies 101: Conventional vs Spectral Flow Cytometry

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   Blog Post

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   Aug. 3, 2023, 1:15 p.m.

   ...Antibody GuideAddgene's collection of ready-to-use recombinant antibodies...

10. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    Blog Post

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    April 19, 2016, 2:30 p.m.

    ...particular type will interact. A protein with a nanomolar Kd will exist primarily as a dimer in cells while...

11. PITChing MMEJ as an Alternative Route for Gene Editing

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    Blog Post

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    Feb. 23, 2016, 3:30 p.m.

    ...which has been difficult to modify due to NHEJ’s dominance over HDR in this species. As CRISPR technology...

12. Which Fluorescent Protein Should I Use?

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    Blog Post

    Published

    May 20, 2014, 2:06 p.m.

    ...effect called FRET (fluorescence [or Förster] resonance energy transfer). FRET occurs when energy transfer...

13. Droplet Digital PCR for AAV Quantitation

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    Blog Post

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    Jan. 16, 2020, 2:18 p.m.

    ...tool for titration and integrity evaluation of recombinant adeno-associated viral vectors." Human Gene Therapy...

14. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    Blog Post

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    Aug. 27, 2020, 1:15 p.m.

    ...Medical Center.  You’ve probably heard of Forster Resonance Energy Transfer (FRET). Through the non-radiative...

15. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    Blog Post

    Published

    Dec. 3, 2019, 11:38 a.m.

    ...In this example we’re going to be looking for recombinant monoclonal antibody plasmids from the James Trimmer...

16. Viral Vectors 101: The Retroviral Lifecycle

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    Blog Post

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    July 27, 2023, 1:15 p.m.

    ...is present in the capsid, as the engineered, recombinant genome is (thankfully) gutted of many essential...

17. Plasmids 101: Colony PCR

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    Blog Post

    Published

    May 12, 2016, 2:30 p.m.

    ...Resources Promega Overview for Screening for Recombinants New England Biolabs Overview of Colony PCR ...

18. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post

    Published

    May 15, 2014, 3:33 p.m.

    ... among the earliest uses of GFP. Fluorescence resonance energy transfer (FRET): This is used to study ...

19. Neuronal labeling with Spaghetti Monster

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    Blog Post

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    Aug. 14, 2018, 12:42 p.m.

    ...neuronal networks. For almost three decades, recombinant Adeno-associated virus (AAV) has been widely ...

20. Finding nucleic acids with SHERLOCK and DETECTR

    Type

    Blog Post

    Published

    April 16, 2020, 1 p.m.

    ...on commercial lateral flow strips, similar to pregnancy tests. With lateral flow strips, the presence ...