We narrowed to 324 results for: pho

1. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

   Type

   Blog Post

   Published

   Nov. 24, 2015, 3:30 p.m.

   ...FPs optimized for oxidizing environments to photoconvertible variants, it seems like GFP is always learning...

2. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

   Type

   Blog Post

   Published

   May 5, 2015, 3:54 p.m.

   ...Louisiana State University. She is a fan of photosynthesis and writes about autotrophs and biochemistry...

3. 5 Tips for Troubleshooting Viral Transductions

   Type

   Blog Post

   Published

   Aug. 11, 2016, 2:23 p.m.

   ...fibronectin-enhanced retroviral transduction of primary human T lymphocytes for adoptive immunotherapy." Acute Leukemias ...

4. Overcoming the Challenges of Lentiviral Production

   Type

   Blog Post

   Published

   Aug. 7, 2018, 12:31 p.m.

   ...transfection method (i.e. polyethyleneimine, calcium phosphate or liposomal methods), the volume of the reaction...

5. Popular Retroviral Vectors and Their Uses in Scientific Research

   Type

   Blog Post

   Published

   July 24, 2018, 12:51 p.m.

   ...genes via infection with a single ecotropic or amphotropic MuLE lentivirus provides significant genetic ...

6. Adenoviral Vector Production and Troubleshooting

   Type

   Blog Post

   Published

   June 14, 2018, 11:44 a.m.

   ...looking at absorbance at 260nm using UV/Vis spectrophotometry. An absorbance of at 260nm corresponds to...

7. Plasmids 101: Modular Cloning

   Type

   Blog Post

   Published

   March 12, 2024, 1:15 p.m.

   ...Level 1 TUs with unique promoters, and the ideal phosphatase (or any modifying enzyme) to pair with it can...

8. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

   Type

   Blog Post

   Published

   March 8, 2017, 3:15 p.m.

   ... Addgene account username. Shipping address and phone number. Plasmid sequences or GenBank files. We encourage...

9. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

   Type

   Blog Post

   Published

   Sept. 26, 2019, 12:50 p.m.

   ...change to large insertions like the addition of a fluorophore or tag. To utilize HDR, a donor template containing...

10. Hot Plasmids February 2024

    Type

    Blog Post

    Published

    Feb. 20, 2024, 2:45 p.m.

    ...Ando et al., 2023). This variant has similar photostability and brightness compared to the original, though...

11. CRISPR in the Clinic

    Type

    Blog Post

    Published

    Nov. 2, 2023, 1:15 p.m.

    ...CRISPR spin. CRISPR CAR-T treatments for leukemia, lymphoma, and some solid tumors are currently in early-...

12. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type

    Blog Post

    Published

    April 6, 2021, 12:30 p.m.

    ...quantification of various intermediate steps, a sizing electrophoretic gel, and qPCR quantification of the final libraries...

13. Hot Plasmids and Viral Preps - March 2021

    Type

    Blog Post

    Published

    March 23, 2021, 1:15 p.m.

    ....   G-baToN can also be applied to other fluorophore/antibody pairs. Because the anti-GFP nanobody...

14. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type

    Blog Post

    Published

    July 23, 2020, 1:20 p.m.

    ...CRISPR-Cas activity. For example, fusing AcrA4II to the photo-sensitive LOV2 domain allows anti-CRISPR activity...

15. How to Prepare for an Industry Interview

    Type

    Blog Post

    Published

    July 9, 2020, 1:15 p.m.

    ...PI of the lab and for one position, I just had a phone interview. I also transitioned between different...

16. Gaining Leadership Skills Volunteering at a Professional Organization

    Type

    Blog Post

    Published

    Aug. 6, 2015, 2:30 p.m.

    ... with other people mostly online and on monthly phone calls. This has its challenges as meeting in person...

17. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type

    Blog Post

    Published

    Jan. 21, 2015, 1:30 p.m.

    ...discuss important issues in person or over the telephone instead of email. As you cannot see a person’s...

18. Degrading DNA with Cascade-Cas3

    Type

    Blog Post

    Published

    Feb. 11, 2025, 2:15 p.m.

    ...Yoshimi, K., Okuzaki, Y., Gee, P., Kunihiro, Y., Sonpho, E., Xu, H., Sasakawa, N., Naito, Y., Nakada, S...

19. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type

    Blog Post

    Published

    April 4, 2019, 12:53 p.m.

    ... of the highly conserved glutamine-fructose-6-phosphate aminotransferase (glmS) gene in the recipient ...

20. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type

    Blog Post

    Published

    Oct. 30, 2018, 1:04 p.m.

    ...form teratomas [11]. iPSCs also possess similar morphologies, growth properties, and express stem cell markers...