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We narrowed to 363 results for: plasmids 101

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Showing: 261 - 280 of 363 results

  1. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...your plasmid; separate plasmid DNA from unwanted cellular “debris”; and then clean-up the plasmid DNA ...debris from the plasmid, and then clean-up the plasmid so that you are left with pure plasmid DNA. Image created...Addgene blog Plasmid Preps: Different Purity, Different Quantities, Different Uses Plasmids 101: Optimizing... help you choose the plasmid prep kit that is right for you. What type of plasmid prep do you need? As...As we covered in a previous post on plasmid preps, not all plasmid preps are the same. Your downstream...arise during the plasmid purification process. One is the possibility of damaging the plasmid DNA itself, ...carrying over contaminants from the plasmid prep process into the final plasmid eluate. Possible contaminants...

  2. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...aptamer downstream of a known promoter. Addgene’s Plasmids 101: Aptamer Fluorophores describes the enrichment...Browse fluorescent protein plasmids and resources See all Jaffrey lab plasmids ... cellular processes by transforming cells with plasmids encoding aptamer-tagged RNA and later introducing...chemical biology 9.10 (2014): 2412-2420. PubMed PMID: 25101481. Filonov, Grigory S., et al. "Broccoli: rapid ...

  3. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...Agarose Method Plasmids 101: Mammalian Vectors Resources on Addgene.org Find mammalian plasmids for your ...

  4. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ... CRISPR User) The Challenges of Cell Culture Plasmids 101: Mammalian Vectors Resources on Addgene.org...Our Protocols Pages Browse Fluorescent Protein Plasmids Find Mammalian Expression Vectors ...of sterile truncated pipet tips (USA Scientific #1011-8410) is also placed in the hood. Finally, a sterile...

  5. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...of Cre-lox. Check out our previous blog post, Plasmids 101: Cre-lox, if you need a quick primer on how ...research? Check out Brainbow plasmids available from Addgene! Find Brainbow Plasmids @Addgene: Brainbow 1.0...1.0, 1.1, 2.1 plasmids Brainbow 3.0, 3.1, 3.2 plasmids References: Transgenic strategies for combinatorial...

  6. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...Gating in Flow Cytometry Antibodies 101: Flow Cytometry Antibodies 101: Validation Resources on addgene.... (tagged or untagged) protein When expression plasmids are available, or the necessary expertise to clone...involves the transient transfection of expression plasmids in a given cell line. For flow cytometry, it is...

  7. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...lentiviral plasmids Additional resources on the Addgene Blog  How to design your CRISPR gRNA CRISPR 101: Ribonucleoprotein... Cell 81, 7, 1553-1565 (2021). https://doi.org/10.1016/j.molcel.2021.02.007 Moroz-Omori, E., Satyapertiwi...

  8. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ... Find CRISPR Plasmids for use in Mammals Check out Our CRISPR Guide Pages Find Plasmids for Mouse Targeting...the target site, and a donor oligonucleotide or plasmid as the repair material if needed (for knock in ..., the gRNA, Cas9, and donor oligonucleotide or plasmid components are brought together and microinjected...provided (in the form of an oligonucleotide or plasmid), precise changes can be made in the genome via...mice carrying the intended mutation. With a donor plasmid, the outcome is less predictable. In our best case.../srep32565 Resources on the Addgene Blog CRISPR 101: Mammalian Expression Systems and Delivery Methods...Viral Vector. iScience 9:286–297 . https://doi.org/10.1016/j.isci.2018.10.030 Qin W, Kutny PM, Maser RS, Dion...

  9. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...the CRISPR 101 eBook Resources on Addgene.org Read the CRISPR guide Find CRISPR plasmids by function...function Browse our entire plasmid collection ... Cell, 184(22), 5635-5652.e29. https://doi.org/10.1016/j.cell.2021.09.018 Nelson, J. W., Randolph, P. ...Target DNA. Cell, 156(5), 935–949. https://doi.org/10.1016/j.cell.2014.02.001 Yan, J., Oyler-Castrillo, P....

  10. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...Vectors 101: Optogenetics Additional resources on Addgene.org Optogenetics Guide Optogenetics Plasmids References...module, which are also available as lentiviral plasmids if you want to make a stable cell line. This design...developing embryo is accessible for injection of plasmids and other materials.    Figure 3: Optogenetic...Guide or browse our collections of optogenetics plasmids – and keep an eye out for future updates to the.... PLoS Genetics, 19(5), e1010765. https://doi.org/10.1371/journal.pgen.1010765.  Berlew, E. E., Yamada...Biology, 32(21), 4660-4674.e6. https://doi.org/10.1016/j.cub.2022.09.010.  Ridley, A. J. (2016). Rho GTPase... in Cell Biology, 36, 103–112. https://doi.org/10.1016/j.ceb.2015.08.005.  Tei, R., Bagde, S. R., Fromme...

  11. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    Published
    Oct. 2, 2025, 1:15 p.m.
    ... from Addgene Luciferase 101 Luciferase Plasmid Collection Promega Plasmid Collection ...cite the original paper but link to plasmids from the Promega Plasmid Collection that express similar constructs...cell and protein analysis products. You can find plasmids deposited by Promega scientists here.  References...

  13. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ... about FLEx vectors Download our Plasmids 101 eBook Read our plasmids blog posts Resources on the Addgene...molecular biology reference Find Cre-lox plasmids at Addgene Browse plasmids at Addgene ...stimuli in the mesolimbic dopamine system." Neuron 101.1 (2019): 133-151. PubMed PMID: 30503173. PubMed Central...the nervous system: a clinical reality." Neuron 101.5 (2019): 839-862. PubMed PMID: 30844402. Juarez, ...

  15. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    Published
    Oct. 6, 2020, 1:15 p.m.
    ...CRISPR 101 blog series Resources on Addene.org Read Addgene's CRISPR Guide Find CRISPR plasmids for your...Expression. Cell 152:1173–1183 . https://doi.org/10.1016/j.cell.2013.02.022 Sajwan S, Mannervik M (2019)...Fluorescence Imaging. Cell 159:635–646 . https://doi.org/10.1016/j.cell.2014.09.039 Zhou H, Liu J, Zhou C, Gao N...

  16. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...comprehensive data sheets on many FP variants Plasmids 101: Green Fluorescent Protein (GFP) Three Review...

  17. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...Blog Read Our CRISPR 101 Posts Read Our Plasmids 101 Posts Read All of Plasmid Technology Posts Resources...amazing plasmid technologies developed by our community of depositors. With over 40,000 plasmids avaliable...amazing new plasmid tools that have come through our doors in 2015. Do you have a favorite new plasmid tool ...system plasmids are available through Addgene and are already very popular. Use these plasmids to increase... can visualize: Cell Structure Plasmids: Cell Structure Plasmids: ER mCherry-Sec61 beta BFP-Sec61...luciferase reporter gene plasmid pGL3 pGL3 luciferase reporter gene plasmids have been used extensively...find all of our Hot Plasmid articles on our website. We update the our Hot Plasmids articles every quarter...

  18. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...Use PASTE can be delivered via a single dose of plasmids, works in non-dividing and primary cells, and ...Precision and Flexibility to CRISPR Engineering CRISPR 101: RNA Editing With Cas13  CRISPR Cheat Sheet Resources...bioRxiv 2021. 11.01.466786; doi: https://doi.org/10.1101/2021.11.01.466786 Additional Resources on the Addgene...

  19. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...! More resources on the Addgene blog Antibodies 101: ELISAs The Four ELISAs and When to Use Them A Control...Antibody collection Addgene's Recombinant Antibody Plasmids collection  ...
Showing: 261 - 280 of 363 results