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Showing: 261 - 280 of 515 results

  1. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    .... During the propagation and/or transformation process, some bacteria will either never receive a copy...antibiotics listed below affect continuous cellular processes (e.g., protein synthesis), while amp only affects...

  2. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...using Gateway technology To better understand the process, we’ll walk through an example experiment where...directly into destination vectors. The cloning process is simple -  no restriction, ligation or gel purification...

  3. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...others for failing to find our work?  I study processes for diagnosing and resolving organizational performance...impact of their contribution. It offers a 5-step process and several examples for creating effective research...

  4. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...You can see some of Joanne’s comments on the AMA process below, but we also wanted to share some thoughts...submission to avoid rousing the trolls. 3. The AMA process was simple and well organized. You can check out...

  5. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...doses and timings of their drug treatments. The process of rising to such challenges involves using knowledge...up-to-date information about the industry, recruiting processes, and can connect you to a community of colleagues...

  6. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...import and export regulations, differing customs processes, and the distances the packages needed to travel...addgene.org. Currently, we’re evaluating our depositor process to identify and reduce barriers for scientists ...

  7. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...transcript by endogenous processing enzymes (Figure 1). To ensure this tRNA processing only occurs on the RNA...

  8. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...sample name. If there was a minor error during processing, the window shows a yellow checked circle. Typically...results. If there are no results or there was a processing error, you will see a red exclamation point in...

  9. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ... a little assistance at some point during the procedure. Be an active partner Everyone’s learning style...training, while others prefer to review their notes, process what they’ve just learned, and ask questions later...

  10. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...to immunoprecipitation and simply refers to the process of anchoring an antibody, often called the capture...protein interactions, and limit high throughput procedures. However, agarose beads are often a more cost-effective...

  11. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...pTargetF system.   Figure 1: The editing process begins with the addition of the sgRNA sequence ...curing, providing users with a complete editing process. The MUCICAT system While the above systems are...

  12. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...the following email from a friend: “I'm in the process of onboarding a new employee who's coming directly...is good. You can experience a real onboarding process. Unless you were really lucky with your academic...

  13. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...capability with the endogenous tRNA-processing system." Proceedings of the National Academy of Sciences...

  14. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ..., studying the genetic underpinnings of these processes has been complicated by the lack of precise genetic...undifferentiated cells at the end of the screening process. However, while the neuronal marker TUJ1 is useful...

  15. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...central nervous system (CNS) orchestrates complex processes enabling organisms to control their movements ...tendency to brightly label somata but not neuronal processes. In contrast, smFPs can label fine neuronal structures...

  16. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...specific RNAs out of a pool of RNAs) or studying RNA processing in live cells. Indeed, groups have utilized dCas13...RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection." Nature 538(7624) (2016):270...

  17. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...a post that goes into the mouse genome editing process in a bit more detail in the coming weeks, but, ...Target Mouse Genes To begin the in vitro screening process, you should first develop primers to the approximately...

  18. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ... that of the 16,000 manuscripts that had been processed since the declaration, only a small fraction (...practicality (given image sizes) but images should be unprocessed and in capture format. This raises another issue...

  19. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...frequency of each variant during the selection process. Each variant is assigned a functional score based...library frequency after selection. Key to this process is the ability to generate full libraries of mutant...

  20. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ... that drain resources from the actual research process. It does take time and effort to implement an ELN...organize their work at every step of the research process will save them significant amounts of time and ...
Showing: 261 - 280 of 515 results