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Showing: 261 - 280 of 597 results

  1. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ... decision before making it, to have an internal review system.  On top of these benefits, potential funding...Addgene blog Read our recent post on Synbio start up Revbio  ...

  2. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...multiple selectable markers. Bob Goldstein’s lab previously used a transgene selection strategy for their...represents another leap forward for the CRISPR/Cas9 revolution!   References Streamlined Genome Engineering...

  3. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...therapeutics for complex genetic diseases. So we’re revisiting this post to look at CRISPR’s potential for use...Technology and its Application in Genetic Diseases: A Review.” Current pharmaceutical biotechnology. https:/...

  4. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...for visualizing genomic loci in living cells. Previous iterations of genomic loci visualization tools...CRISPR-Sirius (Ma et al., 2018). They improved on previous CRISPR-based loci visualization tools by increasing...

  5. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...includes a step that both fragments the DNA and prevents it from forming concatemers later. The "FS" stands..., one was only able to extract libraries from previously fragmented DNA.The general process for library...

  7. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...high-throughput next generation (NGS) sequencing have prevented laboratories from using these methods for routine...inserts and larger targets. The NGS readout also reveals additional information about the presence of non-plasmid...

  8. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ...studies, and has held various editorial roles on review and primary research journals including Trends ...who produce a broad range of primary research and review journals across life and physical science, and ...

  9. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...day in advance. This gives everyone a chance to review the agenda and add anything that is missing.  During...Send around relevant documents, like proposals to review or project plans, before the meeting. At Addgene...

  10. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...brightness, photostability, and so on; see the previous blog post for more discussion of these factors...that is supposed to be extremely bright. In S. cerevisiae, we've tested a number of green and red fluorescent...

  12. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...quantify them with biosensors! In this blog we will review the basics of biosensors and how to use viruses...building blocks. Physiological factors Unlike the previously described sensors, this class measures either...

  13. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...these pesky germs into useful tools. Here, we will review viral basics and the types of viruses used as biological...human subject delivery, as immunogenicity was previously a large barrier for gene therapy. In fact, AAV...

  14. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...science. Well, I’ll give you an example. At my previous company, Ginkgo Bioworks, our software development... the morning! 9:00 AM--Technical implementation review. The developers discuss which front-end and back-end...

  15. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ... other fields or writing grant proposals to be reviewed by interdisciplinary panels.  And highly successful...Our 2018 workshop took place in Boston - you can revisit the activities of our attendees there online with...

  17. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...constructs could lower genome editing efficiency. One previous strategy for multiplexing gRNAs used the RNA nuclease...observed slightly higher mutation rates than they’d previously reported in protoplasts (15% and 9% of target...

  18. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ... a DNA double strand break (DSB) in the genome wherever the user chooses. After the initial cut, the next..., Hsu PD, Lin C-Y, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang...

  19. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ...collaborator Some of these people you meet will review your papers or grants in the future, and don’t ... are talking to is really in your direct area.  Review the conference schedule and attendance list. Plan...

  20. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...post-purification cleavage step to remove the tag in order to prevent it interfering with protein function. In contrast...with the protein of interest (Bornhorst 2000). To prevent this, wash steps often include low concentrations...
Showing: 261 - 280 of 597 results