... vector-free cells Genome integration Need to sequence cells to verify excision didn’t introduce mutations...integration Multiple transfections required Need to sequence to verify there’s no genomic integration Replicating...gene(s) of interest flanked by terminal repeat sequences, which the PB transposase recognizes. Following...can occur at the transposon insertion site, so sequence-verification is required. Non-integrating...

...editing for cleavage-free genomic knock-in of long sequences🎵On the 2nd day of CRISPR, Addgene gave to me:...

...diseases. Spontaneous mutations that change protein sequence affect arginine (Arg) amino acids more frequently...facilitated by CRISPR reagents and other specific gene sequences available from Addgene. Dr. McMurray hopes that...metabolic diseases with severe and life-threatening consequences. They result from defects in different steps...

...CRISPR and RNAi have helped researchers alter DNA sequence and RNA expression - but what if you just want...localization, binding to the appropriate promoter sequence and activation of gene expression. In addition...

...signal sequence and to the mitochondria via citrate synthase’s mitochondrial targeting sequence. Under...

...gene may have errors introduced to the nucleotide sequence during the DNA repair process. The addition of...recombinase catalyzes the recombination of DNA sequences located between two loxP sites. The loxP sites...

...approaches that involve modifying the antibody sequence or that rely on strong protein-protein interactions...Lysine residues are found throughout antibody sequences, up to 80 per antibody (Mueller, et al., 1988)...

...domain can be engineered to target specific DNA sequences while FokI then induces a double strand break.... strategy of using a protein to recognize DNA sequences is not ideal. For the early generation gene editors...protein-based DNA-binding domain with a single-guide RNA sequence.  The Cas proteins, just like its predecessor ...

...plasmid encoding the insert of interest as well as sequences necessary to encapsulate that insert into a viral...plasmid and the packaging plasmid caused the Psi sequence to be placed on the packaging plasmid forming ...composed of the wild-type 5’ and 3’ LTRs, the ψ sequence, a part of the env gene containing the rev response...

...[2]. Delivering dsRNA matching a specific mRNA sequence triggers a cascade of events that results in the...genes not in this collection, they may clone DNA sequences for creating riboprobes and dsRNAs into PR-T4P...

...fortunate to hand-deliver the model, the following sequence of events plays itself out: The researcher breaks...along the double-stranded DNA, recognize a PAM sequence, allow the complementary base pairing between ...

...by gel electrophoresis and/or next-generation sequencing more appropriate? If you already have an end...and sgRNA efficacy by cloning in genome target sequence into EGFP reporter. Target DNA reporter system...

... to ship plasmids or viral vectors containing sequences from rabies and all other lyssa genus members ...

...than BE3, but APOBEC3G displayed less predictable sequence preferences.  The Liu lab also used natural and...develop five new base editors with distinct PAM sequences, expanding the number of available target sites...evolution efforts yielded an editor with more flexible sequence preferences, evoAPOBEC1-BE4max, which is significantly...editing, has allowed scientists to replace entire sequences of varying lengths. Base editing publication ...

... blog post, the team developed a viral genome sequencing strategy to confirm the identity and purity of...

...iteratively generated 3D models and amino acid sequences that got closer and closer to achieving it. Once...

...the PCR product should contain the edited BRAF sequence), and PCR-amplify the BRAF locus from control ... PCR product should contain the wild-type BRAF sequence). Then, mix the PCR products and use temperature... was challenging because you’re limited by the sequence of the human genome. I used the UCSC genome browser...

...EnvA genetic sequences are supplied during production of EnvA-pseudotyped RABV, these sequences are not copied...

...more closely about the wealth of high-throughput sequencing (HTS) data we generate and maintain. It became...begin! Figure 1: Discussing next-generation sequencing at the Addgene bioinformatics journal club! ...

...plasmid. Of course, other follow up methods like sequencing are more definitive, but visual identification...optimization programs to engineer 14 chromoprotein sequences into E. coli BioBrick plasmids (meffRed, eforRed...