Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 430 results for: sequence

Showing: 261 - 280 of 430 results

  1. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ... downstream methods, such as qPCR or next-gen sequencing. ChIP is an incredibly useful application for...suitable for isolated and purified DNA, such as sequencing or qPCR. Yay!  Where’s the challenge in that?...

  3. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...scaffold and cloning sites for inserting the guide sequence (pSpCas9(BB)). The 2A-GFP allows for screening... plasmid for cloning and expressing new shRNA sequences. For more information, see the Addgene’s pLKO.1...

  4. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...are necessary for recognition and cleavage. This sequence flexibility indicates that gRNAs could be incorporated...tRNA-gRNA and tRNA-gRNA-tRNA, with short 5’ spacer sequences preceding gRNAs, to test for gRNA cleavage. gRNAs...

  5. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...heterogeneous when constrained by sequence identities in flanking sequence (“microhomologies”). NHEJ doesn...

  6. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    Published
    Feb. 24, 2015, 7:50 p.m.
    ...libraries will require access to next generation sequencing technology. These libraries are lentiviral ... including a tool for getting optimized guide sequences for activating any human gene, go to http://sam.genome-engineering.org...enabling systematic investigation of the cellular consequences of repressing or inducing individual transcripts...

  7. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ...PEDAR strategy to delete and replace long genomic sequences. On the ninth day of CRISPR, Addgene gave to me...

  8. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...flanked by unique restriction sites so you can swap sequences effortlessly.  We built these plasmids for flexibility... flanking an RNA payload to insert the payload sequence into the genome. The team established that they...elements allowed for scarless insertion — no UTR sequence added to the genome (Figure 4). Figure...

  9. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ... vector-free cells Genome integration Need to sequence cells to verify excision didn’t introduce mutations...integration Multiple transfections required Need to sequence to verify there’s no genomic integration Replicating...gene(s) of interest flanked by terminal repeat sequences, which the PB transposase recognizes. Following...can occur at the transposon insertion site, so sequence-verification is required. Non-integrating...

  10. The 12 Days of CRISPR

    Type
    Blog Post
    Published
    Jan. 3, 2023, 2:15 p.m.
    ...editing for cleavage-free genomic knock-in of long sequences🎵On the 2nd day of CRISPR, Addgene gave to me:...

  12. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    Published
    June 26, 2018, 1:33 p.m.
    ...diseases. Spontaneous mutations that change protein sequence affect arginine (Arg) amino acids more frequently...facilitated by CRISPR reagents and other specific gene sequences available from Addgene. Dr. McMurray hopes that...metabolic diseases with severe and life-threatening consequences. They result from defects in different steps...

  14. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...gene may have errors introduced to the nucleotide sequence during the DNA repair process. The addition of...recombinase catalyzes the recombination of DNA sequences located between two loxP sites. The loxP sites...

  15. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...approaches that involve modifying the antibody sequence or that rely on strong protein-protein interactions...Lysine residues are found throughout antibody sequences, up to 80 per antibody (Mueller, et al., 1988)...

  16. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...domain can be engineered to target specific DNA sequences while FokI then induces a double strand break.... strategy of using a protein to recognize DNA sequences is not ideal. For the early generation gene editors...protein-based DNA-binding domain with a single-guide RNA sequence.  The Cas proteins, just like its predecessor ...

  17. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...plasmid encoding the insert of interest as well as sequences necessary to encapsulate that insert into a viral...plasmid and the packaging plasmid caused the Psi sequence to be placed on the packaging plasmid forming ...composed of the wild-type 5’ and 3’ LTRs, the ψ sequence, a part of the env gene containing the rev response...

  19. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...fortunate to hand-deliver the model, the following sequence of events plays itself out: The researcher breaks...along the double-stranded DNA, recognize a PAM sequence, allow the complementary base pairing between ...

  20. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...by gel electrophoresis and/or next-generation sequencing more appropriate? If you already have an end...and sgRNA efficacy by cloning in genome target sequence into EGFP reporter. Target DNA reporter system...
Showing: 261 - 280 of 430 results