We narrowed to 286 results for: tac
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TypeBlog PostPublishedMay 5, 2020, 1:15 p.m....that contains a fluorophore that is quenched when intact and released when cleaved. Cleavage results in ...
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Transferable Skills Guide: Conflict Resolution
TypeBlog PostPublishedJan. 4, 2022, 2:15 p.m....the past, or how completely different teams are tackling comparable conflicts. Is this level of domain ... -
Tips for Getting a Faculty Position
TypeBlog PostPublishedJuly 20, 2023, 1:15 p.m....your first grant, how you will overcome certain obstacles, alternative approaches, potential collaborations... -
Antibodies 101: Beyond Surface Labeling
TypeBlog PostPublishedJune 27, 2024, 1:15 p.m....more. This process, however, leaves the nucleus intact. If markers of interest are located inside the ... -
An Integrin Antibody Toolkit from IPI
TypeBlog PostPublishedMarch 28, 2024, 1:15 p.m....ligand-binding pocket, their sheer size impeding ligand attachment. The five other IPI rAbs bind RGD-binding integrins... -
CRISPR 101: Validating Your Genome Edit
TypeBlog PostPublishedNov. 3, 2022, 12:15 p.m....can ensure you aren’t looking for a needle in a haystack before going on to generate and screen individual... -
Filming Science Videos in the Age of Social Distancing
TypeBlog PostPublishedMay 20, 2021, 1:15 p.m....mounted a monitor to it. It had an extension cord attached, with the option to mount a battery to it if necessary... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog PostPublishedSept. 14, 2016, 2:30 p.m....research. If you’d ever like to deposit a kit, please contact us at [email protected]. ... -
10 Steps to a Perfect Science Talk
TypeBlog PostPublishedAug. 23, 2016, 2:30 p.m....Describe how you pushed to a solution or overcame obstacles. Take time to consider the order of your narrative... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog PostPublishedSept. 1, 2015, 2:30 p.m....your lab. That in itself is a strong negotiating tactic, and puts you ahead of most start-up P.I.’s. It... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog PostPublishedFeb. 8, 2018, 12:17 p.m....around the world and had specific strategies and tactics for getting the plasmids he wanted. Addgene makes... -
How to Design Your gRNA for CRISPR Genome Editing
TypeBlog PostPublishedSept. 24, 2020, 1:15 p.m....clonal heterogeneity may represent a more serious obstacle to the generation of truly isogenic cell lines... -
Recombinase-based State Machines Enable Order-dependent Logic in vivo
TypeBlog PostPublishedJuly 28, 2016, 2:30 p.m.... perturbation. Also, this method requires live, intact cells; the sequencing and qPCR methods do not. ... -
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
TypeBlog PostPublishedMarch 7, 2017, 3:30 p.m....chicory root, but considering this is a needle in a haystack type of objective, it was surprisingly easy. It... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog PostPublishedNov. 2, 2021, 1:15 p.m....recombination rate of the vector you are using. Contact Addgene ([email protected]), or the supplier of ... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog PostPublishedApril 16, 2024, 1:15 p.m.... strategies include using engineered ligases to attach a ligand to a peptide tag on your protein of interest... -
A History of Genome Engineering in Popular Culture
TypeBlog PostPublishedFeb. 25, 2020, 2:15 p.m....wreaking havoc in a theme park. Released in 1997, GATTACA is an American SciFi film starring Ethan Hawke,... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog PostPublishedJune 28, 2018, 3:01 p.m.... if you have any questions, do not hesitate to contact me at [email protected]. Keep pipetting and... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog PostPublishedJuly 11, 2017, 2:30 p.m.... to target surface proteins to determine which contact points are involved in a studied cellular behavior... -
Antibodies 101: Flow Cytometry
TypeBlog PostPublishedJuly 20, 2021, 1:15 p.m....preserving the protein structure and keeping epitopes intact. It does not, however, permeabilize cells and requires...