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... scientist whose work you are familiar with. You can tweak your elevator pitch slightly if you know a ...you have a poster or talk at the conference, you can end by asking them to come to your poster or talk...your agenda. Some conferences even have apps you can use to build a schedule. If there is a list of poster...this information on the poster) so your visitors can have a DOI to go to. During the conference Arrive...through the poster.” Have a notebook handy so you can jot down notes or questions from your poster viewers...media. Social media Before the conference Networking can be made easier through social media. Use social media...If you could not attend some of the sessions, you can see if others have live-tweeted as well. In addition...

...yourself with a common dilemma. Request the vectors you can find and use them as-is, saving time and effort but...cloning the vectors you actually want. But now you can use the recently developed third option: Fragmid....are the components. Using the handy website, users can simply select the components they want for each module...selection markers. Need fewer components? A spacer can be inserted instead, allowing everything to stay ... individual plasmids and as the Fragmid kit. You can also browse all Fragmid components on the Fragmid...restriction digest, and then plasmid sequencing if you can. To quote Doench, “Whole plasmid sequencing is just...recognition sequence. This means that each component can be designed with an overhang specific to the type...

...ratios with such techniques can be problematic, however. Well designed aptamers can help reduce such background...temperatures, since proteins can denature while aptamers are more stable and can go through cycles of denaturation...bacteria). That range is possible because aptamers can either fold to incorporate their target molecule,...enzymes, such as: Design and selection for targets can be done largely in vitro; Easy and reproducible ...antibodies for diagnostics know how frustrating it can be to get a “bad batch”); Customizable sensitivity...aptamers glow The flexibility of aptamers means they can be used with or as fluorophores in several different...aptabeacons (or aptatags) were born. Aptabeacons can be created in many different ways, but the general...

...viral particles that can transduce cells, which can vary based on target cells and can be altered by freeze... where you can obtain excellent quality, high titer rAAV (including Addgene!), but you can also generate...due to their high density, which can be collected afterwards. You can then remove the density gradient...system, HEK293T cells, expressing Ad E1A and E1B, can be transfected with your transfer plasmid, carrying...titration by qPCR.   Pro tip! An optional media exchange can be performed 12–18 hours post-transfection to help...For certain in vitro and in vivo applications, you can consider using unpurified AAV from the supernatant...Purification To purify your rAAV from your supernatant, you can simply use polyethylene glycol (PEG). We add 25 mL...

...and analysis.  As a rule of thumb, you can assume that qPCR can accurately detect expression differences...differences, and it can even be used to detect a single copy of a transcript. But this sensitivity can sometimes...configurations can help you identify pipetting or evaporation errors. And if time allows, you can fit in a ...statistical significance (Taylor, 2017). With the right conditions and parameters, some people find it can detect... requires significant optimization to ensure the results are correct. Primers and probes can interfere...troublesome, multiplexing your reference gene set can save significant plate space and may be worth the initial...to measure relative quantification of mRNA, which can be used as a proxy measure for differences in protein...

...in non-specific staining can be a challenge in the best of cases. But it can be particularly challenging...(Figure 2B) Some labs have found that you can significantly decrease background staining by mixing your...same levels of endogenous IgGs, so some researchers can get away with species on species staining with no...    Figure 1: Species on species staining can be a problem because the secondary antibody is not... However, directly conjugated primary antibodies can be expensive and may not provide enough signal to...1998). After washing away unbound fragments, you can then apply your primary and secondary antibodies ... Proper tissue processing and sample preparation can also help reduce the levels of endogenous IgGs in...

... fun example and you can find it in our Mentoring for Scientists Guide.  You can use it to clarify and...step you can take is to set goals to work on together and track progress at every meeting. It can take many...impart. An undergraduate can mentor a high school student, a graduate student can mentor an undergraduate...Series. The entire series and additional resources can be downloaded in E-Book format at the end of this...setting to your mentoring relationships, so that you can reap rewards in the form of reaching career development...undergraduate, a postdoc can mentor a graduate student – you get the idea. There are many advantages to being a ... Schedule meetings or calls regularly and don’t cancel, so that you drive progress with regular accountability...

...scientist. In this blog, we’ll delve into strategies you can use to foster innovation. If you identify as a non-creative...yourself a block of time and a quiet space where you can address the issue head on. Prepare for this time ...environment where everyone has their ideas heard.  So how can we assure that all participants feel comfortable ...devil’s advocate is elected, others may feel they can share opposing opinions as well. I am sure we have...goal is to generate unique ideas. Recognize ideas can come any time. Meetings shouldn’t be the only place...ideas are not well received, understand that people can be resistant to change. Ask your peers or your superiors...fixated with the idea of making something the best it can be that it seems impossible to start at all. This...

...the best speaker you can be. Seek out opportunities to practice and over time you can master this important...solutions—public speaking is skill that any scientist can learn.  Any time you give a science talk, you are... added bonus to knowing your audience is that it can help you avoid making mistakes. I once made a joke...a narrative connecting cause and effect. Stories can and should be very simple. The goal is to get the...to include that material, but don’t force it. You can always relate to scientists by explaining how your... real audience Good, even great, speaking skills can be attained by almost anyone with enough practice...previous year. Usually fewer than 10% have done so. How can you expect to deliver a great public presentation...

...are looking, you can either scan the genome yourself for potential gRNA sites, or you can utilise several... or you can order them yourself through whatever DNA supply company you currently use. You can also find...sequencing comes back. Alternatively, if you can afford to wait, you can hold off on genotyping until the pups...targets in that region. The CRISPR Software Matchmaker can be used to determine the pros and cons of using many...genomic fragment containing the sgRNA target sequence can be placed in multi-cloning site (MCS) between EGFP... and sgRNA expression cassette. The target sgRNA can be cloned directionally into the BbsI site. (c) The...sequence surrounding your gRNA. Once amplified, you can insert this region into a pCAG-EGXXFP plasmid using...