We narrowed to 798 results for: KIN

...pretty good about sticking to that. If you email us at noon and then call back at 1PM asking why you haven...Tweeting and texting, actually writing an e-mail or making a phone call is a big commitment when you’re busy...doctoral dissertations. Streamline the question asking process Having thought through the above, don’t...soon as possible!” and I always begin my responses asking for more specifics. We can help you faster if we...how long it will take to get an answer, consider asking to avoid unrealistic expectations. And finally... Eric J. Perkins...

...the intensity of fluorescence signal and enables tracking of single molecules within living cells without...roles in many cellular functions, from endosomal trafficking to signaling pathways to the cell cycle. Good...different chromosomes. The advantage of having this kind of system that works in live cells is that one can...used with your own reporters to decipher their trafficking routes. This collection is now available at Addgene...indicators should possess fast voltage sensing kinetics and have no spectral overlap with the optogenetic...and an endoplasmic reticulum export motif and trafficking sequence for improved targeting to the plasma...CheRiff, exhibits high light sensitivity and fast kinetics. By combining both the voltage indicator, QuasAr...

...hardware sold by the manufacturer. Blocking solutions Your blocking options come in two categories: protein-based...make when designing a western blot.  Psst! Looking for a western blot protocol? Check out Addgene'...antibodies are relatively cheap and easy to source, making it easy to switch from one readout method to another...re running native (non-reduced) proteins or are looking to preserve specific protein modifications, we ...single-percentage gel, which is cheap and useful when you’re looking at proteins all roughly the same size, or a slightly...information. Many antibodies will have a recommended blocking buffer and a recommended concentration. That makes...greater dynamic range than chemiluminescence enzymes, making it easier to accurately visualize proteins of wildly...

...structural biology, Byron Rubin, a crystallographer working with Jane Richardson, provided an initial solution...model. Until then, you can only wonder what I am talking about. How do researchers use physical models of... over and over in their hands. They then start talking to themselves as they compare the features displayed...magical teaching moments. The models function as thinking tools – eliciting questions like “what does this...investigated in their labs. Soon after we began working with educators in our Center for BioMolecular Modeling...complementary DNA strand. And this is why we love working with researchers on a model. The little knowledge...Jennifer Doudna’s new book A Crack in Creation. Making models more useful through flexibility It is common...

...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...undergrads back in grad school. Both were hard-working scientists-in-training who got the job done, but...left him/her with small tasks and s/he was always asking for more work (this is a big hint that you are ...on what you should be doing : writing a grant, thinking about the next project, reaching out to other ...really helpful. “What is the best/worst part of working with X?”  is going to get you what you really want...grateful for the feedback, but at least you can start making changes for the best. Positive feedback is equally...Scientists Series What Makes a Good Mentor? Tips for Working in Industry and Success in Management Resources...

...the conformational dynamics of the motor protein kinesin-1, the Twelvetrees Lab took advantage of the self-labeling...and more photostable than fluorescent proteins, making them ideal for single-molecule fluorescence techniques...deposited an array of constructs for tagging the kinesins KIF5A and KIF5B as well as backbones for generating... will be useful not only for future studies of kinesins but for many other proteins of interest too! Find....A.S., Craggs, T.D., & Twelvetrees A.E. (2024) Kinesin-1 is highly flexible and adopts an open conformation...voltage imaging. A) ASAP5 selected for its improved kinetics and responsivity in screening ASAP3 variants. ...different illumination regimes with fast activation kinetics and high responsivity near the resting membrane...

...researchers learn many transferable skills while working in the lab and by offering advice on areas where... jobs needed to be done (Who is responsible for making the running buffer this week?). Scientists negotiate...approached it as a creative problem-solving exercise? Asking questions like the below can help you understand...you are in the middle of a frustrating situation. Asking for opinions from a neutral third party can be ...offer conflicts with a pre-planned vacation I’m looking forward to. How can I negotiate my starting date... a tenured professor or signing contracts when working for a business development team, negotiation is...strengthen your relationships with others who know that working with you will benefit them too. Ed's note: Salary...

...of His-tagged human kinases in bactera. Listen to Our Segment on The Human Kinase Domain Constructs Kit...We’re breaking into more audio and video on the Addgene Blog and Addgene website. As we push forward ...CRISPR X from Michael Bassik's Lab - A new tool for making diverse, localized point mutations. Listen to Our...Segment on Magneto 2.0 Hot Plasmid 3: The Human Kinase Domain Constructs Kit from John Chodera's Lab -...with Eye Researcher Connie Cepko Get Advice on Picking a Graduate Lab and Project Check Out 5 Science ...

... you’re an oldbe trainee!) can be pretty nerve-wracking. So how can you be the best trainee possible? ...articles on building successful mentor relationships, asking for mentor support, and choosing your mentor. What...And I mean everything! The bare minimum should be taking notes on general protocol steps and other tips ...the moment….but you’ll probably forget and end up asking your mentor later if you don’t write it down. And... to your mentor. Pro tip! Consider physically walking through the protocol, by going to each space in...tell a trainee is understanding and things are ’clicking’ for them. It’s often easiest to train individuals..., What Now? Will You Be My Mentor? Finding and Asking for Mentoring Support   ...

...Mortensen, a PhD candidate at Northeastern University. Working with plants doesn’t always have to be a time-consuming...recently overcome this problem in C. roseus by working with young seedlings and optimizing several other...transient leaf transformation: Check if there are working protocols available for your species This will ... your tissue. Run a control with Agrobacterium lacking the GUS gene since plants can have endogenous GUS-like.... Successful infiltration can often be seen by sinking of the tissue (see Figure 3). This method is probably...GV3101(pMP90), which works well for us. Use a well working vector system This allows you to quickly assemble... you started. Once you have established a well working protocol in your lab, it can become a strong tool...