We narrowed to 432 results for: sequence

... also directly paste sequence data into the appropriate full and partial sequence cells. *Note: If you...strain DH5α. For plasmids with highly repetitive sequences (prone to recombination in bacteria) such as lentiviral...username. Shipping address and phone number. Plasmid sequences or GenBank files. We encourage submission of QUEEN-generated...GenBank files (Mori and Yachie, 2021) but can accept sequence files in any format. Distribution status for your... amino acid change as well as any functional consequences of that change. For example, “Mutated aspartate...

... the iBARs can be detected by next generation sequencing.  In order to avoid a high false-positive rate...gRNAs and to embed these barcodes within the gRNA sequences. This allowed tracing of the performance of each...experiment by counting both the gRNAs and their iBAR sequences. Since the sgRNAiBAR library covers every annotated...stain” DNA. TALE proteins can bind specific DNA sequences which brings the attached fluorescent protein ...

...end-point sequencing assays.  This tool was designed by putting together the coding sequence of a mCherry...base edited cell populations without end-point sequencing assays. These plasmids use “Cas9-mediated adenosine...protein followed by a stop codon and the coding sequence for a green fluorescent protein (GFP). The success...

...recombineering is unsuccessful and the wild-type sequence persists, Cas9 will cleave the gene of interest...have inactivating mutations in the Cas9 or spacer sequence, and these rare events are easily detectable using...effective targeting either the promoter or the coding sequence and the template or non-template strand, among...population based on the presence of specific target sequences (Citorik et al., 2014). These could be genes, ...introduce cytosine-to-thymine substitutions in target sequences (Banno et al., 2018). These substitutions were...approximately a five-base pair window of the target sequence which could be adjusted by changing the sgRNA ...2014) Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials. Nat Biotechnol 32:1146...

...nucleases to transcription factors – to specific sequences of DNA has become commonplace. This ability has...networks. A comparable system for targeting specific sequences of RNA is highly desirable for extending the complexity...possible to produce a protein that binds an alternate sequence. By comparison, Abil explained, the specificity...

...cassettes: plant promoters, N-terminal tags, coding sequences of the gene of interest, C-terminal tags, plant...endonucleases except that they cut at arbitrary sequences a defined distance away from the recognition site...and recognition are independent. “They see one sequence and cut another,” Lohmann said. “It allows us ...

...and PCR genotyping (Sanger sequencing); use primers flanking the tag sequence and expect a size-shift in...pulled-down and later coupled to high-throughput sequencing to identify genome-wide binding patterns of the...facilitates in-frame insertion of tag-encoding sequences at the intended target site. With the widespread...protein, and a donor DNA that contains the tag sequence flanked by homology arms (Figure 1). In the first...donor DNA (ssODN) that contains the tag-encoding sequence and short homology arms (60-70 bp) is added to...ID(s) as an input query, pick crRNA (guide RNA sequence targeting the gene of interest) and ssODN (tag-encoding... promoter region of both the alleles should be sequence-confirmed for "indel-free" knock-in. Another good...

...self-transcribing active regulatory region sequencing) that tests enhancer sequences outside of their endogenous chromatin..., and these sequence changes are subsequently identified through transcriptome sequencing. Importantly... due to the variable availability of enhancer sequences in different chromatin states. Alexander Stark...libraries are cloned downstream of a minimal promoter sequence. Should one of these random stretches of DNA contain...its enrichment over input levels using next gen sequencing. The STARR-seq backbone is available from Addgene... genome (the identity of the genes and target sequences can be found here). To use the library, the authors...cells and surviving “mutant” colonies were deep sequenced to identify the gRNAs that confer protection to...

...target specific sequences, CRISPR/Cas systems rely on a guide RNA complementary to the sequence of interest...recruits transcriptional machinery to specific sequences, causing targeted gene regulation. This can be...either initiation or elongation, depending on which sequence is targeted. Performance dCas9-VP64 activation...Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell 152:...

...target any sequence of DNA. Second, NgAgo target specificity is dictated by a DNA guide sequence rather than...the views of Addgene. Addgene performs Sanger sequencing on select regions of all distributed plasmids...First, unlike Cas9, NgAgo does not require a PAM sequence for target recognition, which gives a researcher...sites in a single experiment. Also, the NGG PAM sequence might not always be available at an optimal distance...