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  2. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ...postdoc association, started a speaker series or biotech club, managed their own blog or podcast, initiated...the board of a professional society, solved a technical problem, had an alter ego as a mad scientist for...

  4. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...especially for life science researchers. Many biotechnology companies are looking for PhD or Master’s level...capabilities, courses and office skills. I removed my techniques and protocols, courses taken during my PhD and...

  5. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...translational approaches for scientific tool and technique development. However, careers outside of the lab...Management Leadership Networking University technology transfer office positions   Market analyses...

  6. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...responsiveness, they showed that iGluSnFR could detect neuronal activity in C. elegans, zebrafish, and...iGluSnFR3 variants should enable scientists to robustly detect signaling events in the brain and beyond. These...

  7. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...transcript targeting by CRISPR-Csm complexes. Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587-022-01649-9... for microscopy by: Susanna Stroik Microscopy techniques have been moving at the speed of light over the...

  8. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...materials available rapidly to scientists (Nature Biotechnology 2020). The continued success of repositories...j.tim.2006.05.007 (2020) Open for outbreaks. Nat Biotechnol 38:377–377. https://doi.org/10.1038/s41587-020...

  10. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ... eat, and rest. Try the Pomodoro Technique or the Flowtime Technique. Co-work virtually. With co-working...

  12. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...viral particles per cell, you may not be able to detect AAV-mediated gene expression in a target cell. ...confirm. Luke Makana Hanley is a Research Team Lab Tech at Addgene working on methods to improve AAV production...

  13. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ... to learn more about this killer illumination technique! What is a photosensitizer? First, for a few ...genetically encoded photosensitizer. Nature Biotechnology,24(1), 95-99. doi:10.1038/nbt1175. PubMed PMID...

  14. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...plasmids: Genetics & Heredity, Cell Biology, Biotechnology & Applied Microbiology, Biochemistry & Molecular...research focuses on a range of topics including biotech and research tools, computing performance, and ...

  15. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...developers’ willingness to make their CRISPR technologies available to the academic research community...Addgene, we’ve helped distribute many of these technologies in plasmid form and are proud to have facilitated...

  17. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...Culture by Gibco Whenever I start to learn a new technique, I try to look for a resource to help me. I’m .... When I started learning proper cell culture technique, I tried to find an app that could help me with...

  18. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...limitations. First, FRET signals are often difficult to detect, and thus quantify, due to small fluorescence changes...induces apoptosis by activating caspase-3, and detected fluorescence within 2 to 5 hours, a time consistent...

  19. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...particularly interested in advanced in vitro models technology. Follow him on twitter @FRE_SCI.   Additional...resources Find protocol videos to teach basic lab techniques Plasmid Land: The plasmid cloning game ...

  20. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ... where the mutation can be placed. With other techniques, when mutations are spaced farther apart, it ...understanding the minimal genome. Many of these techniques require more complex DNA assemblies and improvements...

  21. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...thought that they function as a kind of armor to protect their host from predation (Petroni et al 2000)....the Addgene Blog Read About Other Hot Plasmid Technologies Read Other Synthetic Biology Posts  Suggest ...

  22. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...fluorescent proteins whose spectra overlap, there are techniques, like spectral unmixing, which can be used to...green and red proteins are generally the most detectable and so should be used to tag your least abundant...

  23. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Published
    June 24, 2014, 3:59 p.m.
    ...indicator of transcription within cells, where detection of the reporter protein or its enzymatic activity...regions on gene expression can be determined by detection of the reporter in a specific assay, which ideally...

  24. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...introduction of DNA into bacterial cells); however, the techniques and reagents vary. Plasmid transfection into ...positive selection; however, negative selection techniques can be used in conjunction with positive selection...

  25. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...Tim Miles, PhD, Director of the CLOVER Center at CalTech? If so, you may have submitted a question that ...with no more ado, the questions!  AAV Prep and Technique Q1: Is there any way to deliver a long gene (>...

  26. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...also activating or inhibiting gene expression, detecting neuronal signaling, or manipulating any other ...Others have noted a lack of transduction in catecholamine pathways from the spinal cord (Wang, 2018, Metcalfe...

  27. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...use DNA sequencing or other molecular cloning techniques to determine CRISPR/sgRNA efficiency of an experiment...editing, regulating and targeting genomes. Nature Biotechnology 32:347–355 . https://doi.org/10.1038/nbt.2842...

  30. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...Without these scientists and the support of their technology transfer offices, Addgene would not be able to...educational materials, and provides viral vector technical service (details about virus production protocols...
Showing: 421 - 450 of 684 results