We narrowed to 662 results for: Clu

...once the ligand is removed. Degron tag systems include small molecule assisted shut off (SMASh), auxin-inducible...

...been documented in several autoimmune disorders, including some forms of lupus and rheumatoid arthritis. ...

...Annotate: When creating important documentation, including SOPs or project-related documents, we define new...

...decrease these so-called “off-target effects” have included using a Cas9 nickase variant, lowering Cas9 expression...

... Someone could take on an engineering project including hundreds of genes and regulation and resource ...

...This has been used for a wide range of purposes, including activating an oncogene only in a particular organ...

...page.  CasPhi is 70 kDa Cas protein identified exclusively in huge bacteriophage genomes. It’s active in...

...from pathogenic bacteria’s CRISPR-Cas system. Conclusion CRISPR is a great way to make those genome edits...

...leave academia, and once she joined an investment club and discovered the business side of science, she...

...promoters across these three species, the Roska lab concluded that a promoter’s ability to target a cell class...

...Twitter list contains >300 Twitter literature bots, including a bot for biosensor papers, microbe articles, ...

...these solutions below. Alternatively, companies including ThermoFisher, Millipore Sigma, and Azure Biosystems...

...creating plasmids encoding affinity reagents. These include conventional heavy and light chain recombinant ...

...defined manner. This allows scientists to draw conclusions due to gene expression in a specific brain area...

...Addgene’s CRISPR-based Protein Tagging collection includes systems for tagging mammalian cell lines, Drosophila...

...Successfully infected cells will become round and clump together ~3 days post infection. The amplified virus...

... Find plasmids for other developmental models including flies, nematodes, and zebrafish Find plasmids ...

...Split-BioID’s key advantage over other PPI methods, including BioID, is its spatiotemporal controlled labeling...

...your lab can do to get into the robotics spirit include: Start using electronic multichannel and single...

...example of an individual part, this category also includes promoters, UTRs, terminators, etc. Once selected...

...selected by the ‘quality’ of the gRNA sequence, including propensity for off-targets, secondary structures...

...events-of-interest. Sensors suitable for ER measurements include: CEPIA (additional versions are available to simultaneously...

...replicating and spreading in non-target cells, including yourself as the researcher.   Figure 1:...

...non-profits like Phipps Conservatory or the Brilliant Club and share your knowledge at local schools. Or work...

...pair of loxP sites and a pair of FRT sites and include both Cre and FLP recombinases.   Figure ...

...High throughput transcriptome sequencing methods including work done by the ENCODE project consortium did...

...further the understanding of epigenetics; techniques include chromatin immunoprecipitation, fluorescent in-situ...

... at the Rockefeller University.  His interests include the role of learning and memory in opioid addiction...

...chromatin interaction networks since they are a large cluster of enhancers. CAPTURE 2.0’s multiplexing and high...

... 1: Schematic of Class 1 CRISPR types. Class 1 includes type I with a Cas3 effector, type III with a Cas10...

...effectors allows for a wide variety of screens, including interference, activation, and knockout. Most...

...be produced in multiple different cell types, including plants, mammals, and insects. In this post, we...

...more. The team shares results early and often, including works-in-progress still in need of further study...

...can obtain excellent quality, high titer rAAV (including Addgene!), but you can also generate rAAVs in ...

...qPCR. qPCR is used in a variety of applications including gene expression profiling, studying copy number...

...our recombinase-dependent virus selection to now include Flp-dependent DREADDs viral vectors. Both inhibitory...

...Cancer Research Annual Meeting, you might consider including more background on how your virus of interest ...

... xCas9 2.0-2.6. This second round of evolution included continuous outflow of phage, increasing stringency...

...fluorescent output. The GEVI family of gadgets includes Ace, JEDI, and many more. They can be targeted...

...Many companies have industry postdoc positions, including AstraZeneca, Pfizer, Novartis, Biogen, Amgen, ...