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Showing: 561 - 600 of 879 results

  1. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...with CRIPSR Resources on Addgene.org Find ACVR1 Plasmids Find Plasmids for Disease Research on OurSpecial...and awareness for rare disease and helps patients find the correct treatments, if available. As part of...commercially developed therapeutic antibody that binds Activin A and interferes with inappropriate activin-ACVR1...

  2. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    Published
    March 31, 2015, 4:08 p.m.
    ... bench work. To really be happy, you may need to find a way to do, manage and lead all in one day.  How...is the ability to assess and initiate things independently. Taking initiative doesn’t mean going rogue,...listed a few of my favorites. Additionally, you can find more suggested resources on this topic and get the...Articles on Leadership, also can be purchased individually) ...

  3. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    Published
    April 23, 2020, 1:15 p.m.
    ...Resources on Addgene.org Find AAVs at Addgene Learn more about Addgene's viral service Find protocols for working...tagmentation-based library preparation procedures. This finding eliminates the double-stranding step and reduces... Lecomte E, Tournaire B, Cogné B, Dupont J-B, Lindenbaum P, Martin-Fontaine M, Broucque F, Robin C, Hebben...

  4. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ...scientists using Addgene AAV viral preps to easily find and share useful technical data. These data focus...usage of these tools, which aren’t always easy to find in publications, so this is a much-needed community..., scientists can use the AAV Data Hub to quickly find details they need for planning their next experiments....org Read our AAV guide Browse all  AAV vectors Find ready-to-use AAV preps ...

  5. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    Published
    Jan. 24, 2019, 2:24 p.m.
    ...and visualize. For more details on this procedure, find the protocol here.   Tips and troubleshooting ...Super-resolution mapping of neuronal circuitry with an index-optimized clearing agent." Cell reports14.11 (2016...Meng-Tsen, and Takeshi Imai. "Optical clearing and index matching of tissue samples for High-resolution fluorescence...viral service See our fluorescent protein collection Find AAVs at Addgene ...

  6. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ... but did you know that Addgene also performs independent research? Since launching the viral service in...cells treated with sucrose or other osmolytes to induce hypertonicity display enhanced monoclonal antibody...blog Read our beginner's guide to viral vectors Find all of our viral vector blog posts Download our ...Addgene's viral service Learn about viral production Find viral protocols here ...

  7. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ... try to be as chronological as possible — if you find yourself jumping backwards in time to explain something...your lab or science or your CV has grown as well. Find your audience and fit them You don’t need to explain... paper. Build lots of versions of your story and find the one that fits your audience. Enjoy the story...Check out our blog posts on science communication Find interviews scientists in SciComm careers Check out...

  8. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...can always head over to the Viral Service page to find the full list of what’s available or suggest additional...beginners just starting work with virus. You can find all of our viral vector blog posts here. If you ...    www.addgene.org On the Addgene website you’ll find our updated viral vector pages as well as expanded...Addgene.org Overview of Viral Production at Addgene Find Virus Protocols Browse All Viral Vectors ...

  9. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...potentially rebind and nick the newly ligated DNA. Salt helps prevent topoisomerase I from rebinding, which...the "A" gets added to all PCR products. Keep in mind that Taq polymerase has an error rate of about 1...to Verify Your Plasmid Resources on Addgene.org Find More Plasmid Cloning Protocols Catch Up On Your ...

  10. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...Hybridization Sequence: The region of the primer that binds to the sequence to be amplified (usually 18-21bp... we want to amplify and design primers that will bind to and replicate it. The following image shows the...5'-ATGTGGCATATCTCGAAGTAC-3' for the region that binds the ORF and we will add the EcoRI restriction site... Reference Pages Deposit Your Completed Plasmids Find the Perfect Plasmid for Your Research ...

  11. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...they added flanking loxP sites and a heat-shock inducible Cre to generate a self-excising cassette (SEC)...genetics.115.178335. [Epub ahead of print] PubMed. Find plasmids from this publication at Addgene. Other...: 10.1038/nmeth.2641. Epub 2013 Sep 1. PubMed. Find plasmids from this publication at Addgene. Scalable...genetics.114.170423. Epub 2014 Sep 23. PubMed. Find plasmids from this publication at Addgene. Additional...

  12. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ...having a mentor, developed some strategies for finding mentors and, perhaps, asked someone to support ...identified. Keep a journal to record topics, plans, findings, and progress over the course of an ongoing mentoring...Forced Choice Analysis is a fun example and you can find it in our Mentoring for Scientists Guide.  You can...steps (e.g. Meet 10 PhDs with interesting careers, find a summer internship, etc.). It is helpful to work...

  13. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...motivated to do anything and everything we can to help find a cure -- and quickly. Normally drug discovery is...and help us figure out what is going on there!).  Find the Krogan Lab SARS-CoV-2 plasmids (Note: The Nsp6...over the world, as far flung as the EU, Russia, India and Saudi Arabia -- as well as closer to home in...QBI_UCSF and KroganLab), Instagram, and Facebook. Find more COVID-19 and coronavirus related plasmids and...

  14. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ... monogenetic diseases, the bulk of which you can find below. CRISPR for complex genetic diseases As CRISPR...single-guide RNA targeting the mutant allele (7). An independent team led by Hans Clevers at Hubrecht Institute...fibrosis report. “This is a big advantage over induced pluripotent stem cells, which need to go through...affects multiple organ systems. Nevertheless, the findings are proof-of-principle for use of CRISPR to correct...

  15. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ... has a web and desktop version that works with Windows, Mac, and Linux operating systems.  Zotero  Zotero...desktop based program that’s compatible with Mac, Windows, and Linux.  Mendeley and Zotero have a lot of ...two citation managers. If you want more tips on finding and organizing papers, check out this related blog... blog post: Early Career Researcher Toolbox: Finding Relevant Papers. These are only a few of the many...

  16. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...serotypes, since these 20 ul samples are distributed individually. See our Serotype Testing AAV inventory for ...Addgene blog Read all of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors...

  17. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ... Normally, β10-11 are antiparallel. To prevent binding, the β strands in FlipGFP were redesigned so that...cells expressing FlipGFP with staurosporine, which induces apoptosis by activating caspase-3, and detected...understand these diseases and disorders. You can find the plasmids for FlipGFP and FlipCherry at Addgene...Addgene.org Browse the fluorescent protein collection Find empty backbones for fluorescent protein fusions ...

  18. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...scientists have yet to decipher the mechanistic aspects behind these improvements. What have exosome AAVs achieved...Addgene blog Read all of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors...

  19. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ... my head and left me thinking: I wish I had this kind of experience earlier! After all, students build...PMID: 30083609. PubMed Central PMCID: PMC6070313. Find the plasmids from this publication at Addgene! ... Additional Resources on the Addgene Blog Find planarian tools for teaching developmental biology Use... Check out our molecular biology reference pages Find plasmids from the BioBits BrightTM publication here...

  20. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

    Type
    Blog Post
    Published
    July 21, 2017, 12:48 p.m.
    ...or off. Gal4 is a transcriptional activator that binds to UAS enhancer sequences found in DNA. It then ...recruits transcription machinery to the site to induce gene expression. Thus, genes and siRNA encoded ...neuronal compartment, you could express them individually or simultaneously. Do note for control purposes... much protein as overexpression of each gene individually. Applications of the Gal4/UAS system There are...

  21. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...DNA is harvested, much like one would prep an individual plasmid. However, lots of library DNA is needed...obtain about 100 to 1,000 bacterial colonies per individual plasmid in the library. Bacterial growth must...representing perfect uniformity (see Figure 3A). Addgene finds that this representation is most easily understood...amplification looks like, but by looking at these kinds of graphs, making sure few/no plasmids have dropped...

  22. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...CD8 or CD4 epitopes, as well as their potential binding affinity to major histocompatibility complexes ...not yet possible for these algorithms to model individually-specific factors, such as previous exposures...prediction of immunogenicity is to validate the findings from the in silico assessments, as well as analyze...dentritic cells or macrophages through cytokine induction (Groell et.al, 2018). Thus, microscopic in-vitro...

  23. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ... Resources on the Addgene Wesbite Find Reprogramming Plasmids Find Other Hot Plasmid Tools Visit Our ...paper showing that it was possible to generate induced pluripotent stem cells (iPSCs) capable of forming...like an understatement. Multiple speakers from top minds in the stem cell field have described the Yamanaka...

  24. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ... and higher pH. We initially used this screen to find R body mutants that couldn’t extend at a normal ...the ciliate. It’s thought that they function as a kind of armor to protect their host from predation (Petroni....1021/acssynbio.5b00237. PubMed PMID: 26814170. Find Plasmids from This Publication at Addgene 4. Petroni...Resources on Addgene.org Read our Hot Plasmids Articles Find Plasmids for Bacterial Expression ...

  25. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...ssDNA molecules in vitro. Figure 1: Cas9 binding to the cleaved DNA duplex. Image from Richardson...diseases in which sequence replacement holds some kind of fitness advantage but error-prone repair of the...on Your HDR Basics Learn How to Design Your gRNA Find the Ideal CRISPR Software for Your Needs Resources... on Your CRISPR Background with Our CRISPR Guide Find CRISPR Plasmids for Your Research Check out Our ...

  26. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ... Podcast.       Resources at Addgene Find CRISPR resources Find CRISPR pooled libraries Learn about other...editing and screening) such that thousands of individual edits can be studied quickly. Involvement with.... the nonprofit, sharing ethic that Addgene has behind it is something that very much resonates with me...

  27. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...necessary for most ChIP experiences, but the increased binding can mask the epitopes antibodies recognize. It ...In the case of histone modifying proteins, which bind quite strongly to DNA, crosslinking may not be necessary...to the DNA, depending on how tightly the proteins bind natively. But the amount of cross-linking can affect...Introduction to Antibodies Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene...

  28. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...the Fc portion of different antibody isotypes. Binding to complement system proteins, which can initiate...chain domain as your primary antibody but doesn’t bind your antigen of interest. Isotype controls are used...staining you see is due to target-specific antibody binding rather than nonspecific background staining. Isotype...structure, which means each IgM molecule has a binding capacity or valency of 10 antigens. An extra constant...

  29. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    Published
    Feb. 19, 2019, 1:36 p.m.
    ...creates more breakthrough and innovation. With any kind of open platform, there is the potential for increased...general auditor. I oversee a department of 20 individuals that help the plan manage risk. My job and my...LLP, auditing insurance, finance and high-tech industries. He has earned both an MBA in finance and a B.S...the other members of Addgene's Board of Directors Find careers at Addgene ...

  30. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...post is designed with beginners in mind, there’s some great reminders for Twitter pros as well, like tip...tips on using Twitter as a scientist. You can also find Dr. Page and Hello PhD on Twitter: @ThePurplePage...capture your interest during that week.” You can also find Dr. Knoepfler on Twitter: @pknoepfle 3. "Social ...

  31. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    Published
    May 11, 2017, 2:30 p.m.
    ...mutated cancer genes are rare and most of the individual variants found in tumors are exceedingly rare...But, often these methods don’t agree. Confusing indeed! We've developed a new plasmid toolkit, the Broad...approach. Also, in principle, these experiments could find correlations between variants in a gene of poorly... next-generation of cancer patients. We hope you find these new cancer variant plasmid collections useful...

  32. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...(i.e. Csy4 cleavage sequence, tRNAs, multiple individual promoters).  spCas9 and its gRNAs are also larger...suitable substrate for Cpf1. This means Cpf1 can bind and cleave the lentiviral RNA, preventing packaging... with Cpf1 and GFP.  Of these neurons, ~15% had indels at all 3 targeted loci.  See the chart below for...Addgene.org Browse Our CRISPR Multiplexing Resources Find CRISPR Plasmids for Your Research Catch up on Your...

  33. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...You’ve committed to the daunting task of finding new factors in your biological system of interest, the...are assessed, regardless of whether they enhance, hinder, or have no effect on the process you are interested...everything else dies off. For a screen, you would individually treat cells with many different drugs and then... forward screens. A given panel of drugs are individually administered and then outcomes are measured....

  34. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ...replication, repairing mismatched basepairs or small indels that occur during DNA synthesis, and protomoting...processes (for example Dam and Dcm methylases) are independent from the restriction modification systems, yet...non-mutated and methylated template is destroyed leaving behind a pool of mutated plasmids which can later be verified...that is unmethylated at those sites. Please keep in mind that dam-/dcm- strains may have an increased rate...

  35. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...manuscript to make resources easier to identify, find back, and share. Depending on the type of manuscript...including F1000Research, also ask for the raw data behind the figures and results. This is a relatively new...researchers to access the data and make up their own minds on whether the data analysis has been of good quality...accepted for publication and is, in most cases, blind (meaning you don’t know who the reviewers are). ...

  36. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...manuscripts shared by the authors on a public server independent from peer-reviewed publishing in a journal.  ...is usually a confidential process among a few individuals.  Early-career researchers especially enjoy the...an updated preprint, further increasing their findability. Now, go out there and finish that experiment...10.1371/journal.pbio.3000151 Sever R, Roeder T, Hindle S, Sussman L, Black K-J, Argentine J, Manos W, ...

  37. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ... Five: Share your results.  Does this process remind you of anything? Perhaps the Scientific Method?...expected to write up a report to communicate your findings to your colleagues. So perhaps a better definition...time in a lab learning techniques and taking on independent projects, you will inevitably develop problem...improve. The next time you face a problem, try to be mindful of how you work through it. Ask yourself if you...

  38. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...neurons are likely the first thing that comes to mind. However, ion-based communication is not just for...reporting and genetically encoded fluorescent voltage indicators (GEVI) are ideal tools to track bioelectric states...channelrhodopsin used as a sensitive fluorescent voltage indicator with near-complete desensitization in continuous...BLINK2 potassium channel - An optimized blue-light induced synthetic potassium channel. Upon activation, it...

  39. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ... on the Addgene Blog Find tips for online social networking for scientists Find tools for using social...profile is absolutely necessary. If people can’t find you on LinkedIn, you will lose opportunities and...for politics, family updates, or click bait of any kind. For more on inappropriate content see this article...

  40. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...Addgene.org Find protocols for plasmid cloning Read our molecular biology reference Find how-to videos..., Gibson Assembly, Golden Gate etc.), and try to find the simplest way to do it (i.e. avoid assembling...cloning strategy: Restriction enzyme digestion Be mindful of the restriction enzymes you chose. Make sure...
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