Addgene

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Oh, The Places You Can Go: Careers in Science Communication - Product Development

Type

Blog Post

Published

Oct. 18, 2017, 1 p.m.

... husband’s postdoc. Colorado is growing in the biotech sphere, and growing in the science sphere, but ...
SunTag and Fluorescent Imaging

Type

Blog Post

Published

March 28, 2017, 2:30 p.m.

... into the nucleus. In developing their SunTag technology, the Vale and Weissman labs took this biological...
Addgene at the ASM Microbe 2016 Conference

Type

Blog Post

Published

July 7, 2016, 2:30 p.m.

...doing creating well-optimized CRISPR screening technology. Addgene got several shout-outs for making popular...
Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

Type

Blog Post

Published

June 11, 2019, 1:16 p.m.

...I was focused on writing about new scientific techniques and cool plasmids. Creating graphics were usually...
Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

Type

Blog Post

Published

July 2, 2015, 8:37 p.m.

... the skills researchers gain doing PhDs (i.e. technical writing, teaching, presenting, analytical thinking...
Sleeping Beauty Awakens for Genome Engineering

Type

Blog Post

Published

June 30, 2015, 2 p.m.

...rapidly generate stable transgenic cell lines. Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821. PubMed...
Management for Scientists: Managing vs. Leading

Type

Blog Post

Published

March 31, 2015, 4:08 p.m.

...Their work involves coordination and can require technical mastery. Scientists need to be doers for a long...
Management for Scientists: What Makes a Good Manager Anyway?

Type

Blog Post

Published

Dec. 9, 2014, 2:22 p.m.

...described by Sebastiano Massaro in his Nature Biotechnology article “Managing knowledge-intensive workers...
The Stingy Scientist: How the Baby Gel Box Was Born

Type

Blog Post

Published

April 15, 2014, 3:11 p.m.

...used this procedure daily before the advent of techniques like PCR and DNA synthesis. Most molecular biologists...
Plasmids 101: Antibiotic Resistance Genes

Type

Blog Post

Published

Jan. 30, 2014, 3:29 p.m.

...problems at once – it allows a scientist to easily detect plasmid-containing bacteria when the cells are ...
Choosing a Good Mentor for Scientists

Type

Blog Post

Published

Jan. 16, 2014, 2:56 p.m.

...data, solve research problems, develop general technical proficiency, communicate your work, maintain ethical...
What the HEK?

Type

Blog Post

Published

April 18, 2023, 1:15 p.m.

...Recombinant Proteins and Viral Vectors. Front Bioeng Biotechnol. (2021) (9):796991. DOI: 10.3389/fbioe.2021.796991...
Addgene Begins Distribution of Recombinant Antibodies

Type

Blog Post

Published

March 28, 2022, 4:30 p.m.

...Brain Research Through Advancing Innovative Neurotechnologies® (BRAIN) Initiative. Our first collection ...
RUBY-Red Siliques

Type

Blog Post

Published

March 1, 2022, 2:15 p.m.

... for a wide variety of applications. But some techniques require you to select against the presence of...
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

Type

Blog Post

Published

June 22, 2021, 1:15 p.m.

...unable to get into the target cells and this can be detected via loss of luminescence. B.1.351 variant...
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

Type

Blog Post

Published

June 17, 2021, 1:17 p.m.

... “AraC is widely used in synthetic biology, biotechnology, and almost every microbiology lab,” says Di...
Meet the New Addgene Board Members!

Type

Blog Post

Published

April 28, 2020, 1:14 p.m.

... development, vaccine development, and assay technologies makes her a perfect fit for Addgene. She was...
New and Upcoming Viral Vectors - December 2019

Type

Blog Post

Published

Dec. 19, 2019, 2:40 p.m.

...ubiquitous expression. (For more details on this technology, see our blog post Overview of AAV Encoded Calcium...
New and Upcoming Viral Vectors - Spring 2019

Type

Blog Post

Published

March 26, 2019, 1:40 p.m.

...KGC-GFP-ER2]) of this membrane-embedding voltage detector. Find Archon1 and its cre-dependent version at...
Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

Type

Blog Post

Published

Nov. 8, 2018, 3:47 p.m.

...opportunity to study it and see what happens when technological change happens really fast,” Thompson says. ...
Using Addgene's New Viral Service for Your Research

Type

Blog Post

Published

Nov. 17, 2016, 3:30 p.m.

...the right cells using your particular delivery technique and conditions. Finally, we have retrograde AAV...
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

Type

Blog Post

Published

Aug. 8, 2019, 1:19 p.m.

...salt-stress induced responses in Root System Architecture of Arabidopsis HapMap population in Amsterdam...
Plasmid Cloning by PCR

Type

Blog Post

Published

March 29, 2016, 2:30 p.m.

...on the Addgene Blog Learn About Other Cloning Techniques Catch Up on All Things Plasmid with Our Plasmids...
Hot Plasmids: FRET-Based Biosensors

Type

Blog Post

Published

May 6, 2014, 1:07 p.m.

...not known.” Transition Metals Maarten Merkx of Technische Universiteit Eindhoven has similar interest in...
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

Type

Blog Post

Published

March 4, 2014, 4:49 p.m.

...philosophical implications. As an example, a group at Caltech used an optical fiber together with optogenetic...
Viral Vectors 101: Inverted Terminal Repeats

Type

Blog Post

Published

Oct. 19, 2023, 1:15 p.m.

..., small deletions and point mutations will go undetected by restriction digest methods. Resources...
Scientists Map the SARS-CoV-2-Human Interaction Network

Type

Blog Post

Published

April 9, 2020, 1:15 p.m.

...constructs are tagged with a 2xStrep-tag for easy detection and affinity purification. All but one are in ...
What Good Citizenship Can Do for Reproducibility in Science

Type

Blog Post

Published

Nov. 7, 2019, 2:18 p.m.

...are often very subtle, and it can take a lot of detective work to figure them out. But, when they come to...
Improving Transduction Efficiency with Exosome AAVs

Type

Blog Post

Published

March 14, 2019, 1:54 p.m.

...association of AAV particles with membrane vesicles protects them from antibody recognition and inactivation...
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

Type

Blog Post

Published

Nov. 20, 2018, 8:17 p.m.

...Bourges for editing this post and sharing her technical expertise in persistent identifiers. Additional...
Creating accessible biology activities in schools with BioBits

Type

Blog Post

Published

Aug. 2, 2018, 12:56 p.m.

...Browse our protocol videos to learn simple lab techniques Check out our molecular biology reference pages...
FPbase: A new community-editable fluorescent protein database

Type

Blog Post

Published

May 16, 2018, 1 p.m.

... on the Addgene Blog Fluorescence Microscopy Techniques - Which is best for me? A Practical Approach ...
Predicting Adverse Reactions to Monoclonal Antibody Drugs

Type

Blog Post

Published

Feb. 13, 2024, 2:15 p.m.

...of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 130, 128–142. https://doi.org/10.1016...
Summer SciComm Series: A PhD in Science Communication

Type

Blog Post

Published

Aug. 23, 2022, 1:15 p.m.

... remains inaccessible, behind paywalls and in technical jargon that even scientists in neighbouring disciplines...
New and Upcoming Viral Vectors - September 2019

Type

Blog Post

Published

Sept. 12, 2019, 1 p.m.

...of calcium dynamics. (For more details on this technology, see our blog post Overview of AAV Encoded Calcium...
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

Type

Blog Post

Published

Sept. 3, 2019, 1:15 p.m.

... tagged proteins, a common problem of the AID technology (Yesbolatova et al., 2019). Find the mAID tagging...
Viral Vectors 101: Viral Vector Elements

Type

Blog Post

Published

July 25, 2023, 1 p.m.

...immune response to the virus to allow it to evade detection by the host. Of note, recombinant adenovirus is...
Viral Vectors 101: Biosensors

Type

Blog Post

Published

July 6, 2023, 1 p.m.

...as FRET-based biosensors have been engineered to detect a wide array of neurotransmitters ranging from ...
Viral Vectors 101: Viral Applications

Type

Blog Post

Published

April 13, 2023, 1:15 p.m.

...over a hundred years ago. Since then, medical technology has changed a lot, but viruses have stuck around...
Plasmids 101: Screens vs. Selections

Type

Blog Post

Published

Nov. 10, 2022, 2:15 p.m.

...added baseline component. Alternative screening techniques While CRISPR and drugs dominate the screening...

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