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...our best guess). Follow @Addgene, use the hashtag #DeckTheLab, and tag @Addgene in the post! In January...It’s finally December, and here at Addgene HQ in Massachusetts that means it’s time for near-freezing...winner, you’ll receive a winter care package from Addgene.  Here are the details:   Contest 1: Deck the ...CVRHutchinson #SciArt #STEAM #WinterWonderlab @Addgene Make your own here!: https://t.co/AnltWfKuQg pic.twitter.com...however you celebrate! And if you want to see how Addgene celebrates and decorates, follow us on Twitter ...

...experiments, let us know (datahub@addgene.org) and we might just send you a new Addgene Data Hub t-shirt!      ... out the redesigned, revamped, and re-launched Addgene AAV Data Hub, the place for scientists to share...curated collection of community submissions for Addgene and CLOVER AAV experiments - and we've updated ...Submitting to the Data Hub If you've ever validated an Addgene or CLOVER AAV, you can (and dare we say, should...step-by-step submission guide or email datahub@addgene.org to learn more. A great submission includes: ...

...quietly learning about Addgene’s past and present, but the real promise of Addgene lies in our future. As...Addgene saw big changes in 2022, from the launch of a new material to the redesign of a community resource...collection, which includes both neuroscience and general-use antibodies, is available in both trial- and...organization. One thing that will remain the same is Addgene’s commitment to supporting the research community...number of people. I look forward to working with Addgene colleagues, collaborators, partners, and the larger...

...accessible to the general public both in terms of cost and content level. Sponsorship from Addgene and Harvard... Here at Addgene, we’re dedicated to advancing and sharing science! In association with the Harvard graduate...DayCon. DayCon is a one-day conference aimed at the general public that provides an accessible introduction...introductory lectures targeting three broad concepts: the genetic basis of disease, cancer, and viral infections.... do you share your science with the community? Addgene would love to hear about your efforts to engage...

... Biology Resources on the Addgene Blog Deposit your plasmids with Addgene and make your work more accessible...copes with the growing amounts of digital data generated in labs. While many labs approach the situation...are available on the market today, ranging from general note taking tools, to more scientific field-based...One comment reflected the desire for a flexible generic solution, rather than an ELN designed for a specific...From pictures to excel sheets, researchers today generate vast amounts of digital data during their work...Twitter @scinoteELN Additional Resources on the Addgene Blog Learn about more tools for reproducibility...

...Are the shots clear enough for the audience? As Addgene’s Science Video Production Specialist, I’m constantly...March 2020, just four months after I started with Addgene, I was about to have a check-in with my boss Jennifer...Cycle 3: A Return to HQ Figure 3: Even Blugene is practicing social distancing and wearing their...traditional solution was completely out of reach for Addgene.  So, onto research! I needed to design a system... needed to figure out a microphone situation. Generally, I record into an external recorder using a lav...the Lab Design: The lab is the beating heart of Addgene. It’s why we exist, storing plasmids, and distributing... out all of the written and video protocols at Addgene? If you need help in the lab, we have you covered...

...Addgene recently attended and sponsored the Future of Research (FOR) Boston Symposium 2015. I personally...fun and surprisingly exhausting experience. As Addgene believes many of the topics discussed during the...are many companies and organizations (including Addgene), that need people who are data oriented, scientifically...skills beyond those required at the lab bench. At Addgene we try to do our part in boosting awareness of ...write for a university publication, improving your general writing skills will help you in your academic publishing...problems within the research environment itself. At Addgene we hope that all parties involved in making academic...Publications of Interest Can Be Found Here Resources at Addgene  Read David Riglar's FOR Boston 2015 Blog Post...

... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR... optimized for Burkholderia, they identified 67 genera of Proteobacteria that this toolkit might be used...TadA) to a catalytically impaired Streptococcus pyogenes Cas9. They called this editor Target-ACE (adenine...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...more information. Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

...thread ⬇️⬇️⬇️ — Addgene (@Addgene) April 15, 2020   Additional resources on the Addgene blog Read about...essential research into COVID-19 (like here at Addgene!), and new databases are supporting their access...” said Guillaume Bourque, a Professor in Human Genetics at McGill University. “There is a need to pool...guide for external collaborations Resources on Addgene.org Browse our COVID-19 plasmid collection ...

...people and society. Future generations of IMP Motivating future generations is key to the success of this...from international financial struggle, our new generations feel thrown, to sink or swim on their own, into...knowledge, experience, and new professional capacity generated during this process result directly in positive... growing demand from universities and future generations of professionals has made possible a significant...School, where she studies age-related macular degeneration. She is also a blogger who writes posts for ...Advancemnet of Higher Education website Resources at Addgene Read Other Career Blog Posts Read the Networking...

...sometimes unclear or missed altogether. Emailing Addgene might not be as high on the published-paper-celebration...in this status — a win-win situation for all. Addgene will release plasmids on hold if they are clearly...celebrate!), send us a quick email at deposit@addgene.org with the citation and your deposit or plasmid...distribute unpublished materials. What about preprints? Addgene supports preprints and encourages authors to share... for reading, and for depositing plasmids with Addgene! We'll continue to review our policies to make ...website, Help Center, or email us at deposit@addgene.org. ...

...Additional Resources on Addgene.org Brush Up on Your CRISPR Background with Addgene’s CRISPR Guide Page Find... heat loving) bacteria, such as Streptococcus pyogene’s SpCas9. These Cas9 proteins don’t work well at...try, as always, the plasmids are available from Addgene. Happy CRISPRing! References Harrington, Lucas...bioRxiv (2017): 138867. Additional Resources on the Addgene Blog Read about CRISPR Methods for Bacterial Genome... Find CRISPR plasmids at Addgene ...

...involves amine-reactive biotin derivatives that generate covalent bonds with positively-charged amino acids...solution was to exploit the best of both worlds. We generated a plasmid that produces biotin ligase and a protein...the biotin ligase BirA backbone (Eric Campeau, Addgene plasmid #26624) was unsuccessful. Nothing more ...pMal-T-Avi-His/BirA plasmid now available through Addgene. We have successfully used this plasmid to biotinylate...cell signaling.     Additional Resources on the Addgene Blog Check out other Hot Plasmids Find Additional...Posts on Plasmid Technologies Resources at Addgene.org Learn about Popular Plasmids in the Repository...

...about pseudotyping Resources on Addgene.org Find plasmids in Addgene's COVID-19 collection Find SARS-COV...coronavirus and SARS-CoV. These two viruses are quite genetically distinct from SARS-CoV-2 and its variants. Yet...Balazs lab was quick to deposit their plasmids at Addgene to accelerate ongoing COVID-19 research. “I hope...j.cell.2021.03.013 Additional resources on the Addgene blog Browse all COVID-19 related blog posts Learn...-COV-2 pseudotype virus plasmids from Addgene ...

...language is ... The general representation of data is... The figures are ... In general, I think that this...language in the first (and clunky) version of my general comments and adjust them if my opinion changed ...can be deposited in the specific repositories (Addgene, WormBase, Arabidopsis.info). These repositories...PMCID: PMC4528400. Additional resources on the Addgene blog Find blog posts on scientific sharing Get...tips on writing a review article Resources on Addgene.org Learn about depositing plasmids pre-publication...

...writing for a general audience. The tool highlights jargon terms or words rarely used in general writing in... defining terms. The phrases in Phrasebank are generic, so they need to be edited to suit your particular...desired formatting style. This tool is great for generating citations for a few papers at a time. Mendeley...method in grad school Additional resources on the Addgene blog: Tips for writing scientific manuscripts:...writing a scientific review article   Write for the Addgene Blog ...

... Cytometry Resources on Addgene.org Addgene's Antibody Guide Browse Addgene's Antibody Collection     ...single color controls On a flow cytometer, you generally want your positive cells to read between 104 and...isotype controls, while others don’t. However, the general consensus seems to be that if you are looking for...Harvard Medical School.   More resources on the Addgene blog Introduction to Gating in Flow Cytometry Antibodies...

...Addgene was built on the concept of accessibility: making plasmids more accessible to scientists around...the next twenty years. But learn we did! From Addgene's very beginning, we had to consider digital accessibility... accessibility on our website.  As members of Addgene’s content team, we — Jo, Brook, and Angela — most... in a poster compared to an online abstract or Addgene blog post.  Here, we are presenting this image ... with the rest of the workshop.  For Figure 1, Addgene chose this alt text: "The left panel is a profile...writing!   Resources Additional resources on the Addgene blog Five Steps to Writing Grant Proposals to Grad...

...communication and epigenetics. Follow her on twitter @kc_palozola.     Additional Resources on the Addgene Blog ...keep track of the files that I was continuously generating.  I quickly found that there is no one way to...physical lab notebook, along with any figures generated from the analyses: Goal: Before you begin, briefly...control your commonly used scripts: If you edit a general purpose script for a single use, save the script...Software Matchmaker Additional Resources on Addgene.org Find Protocols for Your Next Experiment Find...

...Mess in IHC Resources on Addgene.org  Browse ready-to-use antibodies at Addgene...visualize a Y-shaped protein reminiscent of the Addgene mascot Abi? If so, you are not alone. Full-sized... divide up the different regions, both through genetic and proteolytic approaches. This comes in handy...when that might be later). Find scFv plasmids at Addgene Depending on how an antibody is broken up, there...j.crmeth.2022.100301  Additional resources on the Addgene blog: Introduction to AntibodiesSingle Chain Fragment...

...interaction.  In as little was one minute BASU generated a strong streptavidin signal (~250-fold enrichment...point, but one minute of labeling with BASU still generated a >30-fold enrichment compared to E. coli’s BirA...host protein critical to Zika replication. Human genetic disease. RaPID can also be used to test how RNA... answer? The RaPID plasmids are available from Addgene! References Ramanathan, M., Majzoub, K., Rao, ...PMCID: PMC4054858.   Additional Resources on the Addgene Blog Study Protein-DNA Interactions with CUT&RUN...resources for studying chromatin Resources on Addgene.org Find Split-BioID plasmids Find CRISPR plasmids...

... Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene  ...antibodies work?  Secondary antibodies are antibodies generated against antibody isotypes from a specific species...classes, it is possible to get secondary antibodies generated against isotype subclasses, such as mouse IgG2...my goat anti-rabbit?!”*  *a secondary antibody generated in goat against rabbit immunoglobulins. References...References and Resources More resources on Addgene’s blog Antibodies 101: Introduction to Immunofluorescence...

...workshops and organizational skills. Meanwhile, Addgene was also thinking about ways to address irreproducibility... to Teytelman’s connection with Joanne Kamens, Addgene also had the opportunity to contribute to this ...being adopted by a wider group of researchers. Generating material to empower others to practice and teach...anyone could present the material. For example, Addgene’s contributions by Tyler Ford included reagent sharing...Tsang for editing. Additional resources on the Addgene blog: Check out this guide on Transferable Skills...workshops Levelling Up: Reproducibility for Everyone Addgene: a global resource working to enhance scientific...

... Resources on Addgene.org Unconjugated antibodies at AddgeneAntibody plasmids at Addgene... functionality of the conjugate. For example, Addgene’s antibodies are provided in a buffer with the antimicrobial... Schibli R (2015) Antibody Conjugates: From Heterogeneous Populations to Defined Reagents. Antibodies ...10.1038/nmeth.4380  Additional resources on the Addgene blog Antibodies 101: Buffers, Storage, and ConjugatesAntibodies...

...placed on the Addgene website, it sets off a chain reaction of events throughout all of Addgene - within the... of thousands of plasmids a day, like we do at Addgene, pen and paper will fail you. The benefits of lab...fun too. Kris Shamloo is a Software Engineer at Addgene and is interested in writing software tools to ...krisshamloo.com     Additional Resources on the Addgene Blog Learn about How We Use Barcodes in the Lab...Track Your Experiments Additional Resources on Addgene.org Find Plasmids for Your Research ...

...monoclonal antibodies; Addgene collaborates with these companies to create and provide general availability of...recombinant forms (rAbs). mAbs and rAbs have higher homogeneity than polyclonal antibodies because of their immunoglobin...that can be produced in large “copies.” This homogeneity circumvents the batch-to-batch variability found...that the primary antibody is very weak or with general non-specific binding. If the sample differs from...10.1371/journal.pbio.1002165 More resources on the Addgene blog Antibodies 101: ValidationSelecting the Right...

...interesting discoveries. You’ve analyzed the data and generated beautiful figures. And, you can’t wait to tell...need to first make your poster. While a poster generally contains the same sections as a primary research...references and contact information, and it is generally acceptable for the font here to be much smaller...it’s good to let your ingenuity shine, and you generally have free reign over how you put your poster together... as rows. It can be more catchy because it is generally less common and will stand out; however, be careful...make it your own! Additional resources on the Addgene blog Check out this blog post on navigating the...

...skill set Resources on the Addgene website Become of a member of the Addgene depositor community ...a conference, be sure to practice beforehand. Generally, two types of people will visit your poster: those...the tweep up (and for the entire conference, in general). After the conference Review your favorite conference... considered networking, but here are some more general tips. Before the conference Networking is key even...your scientific conference? Resources on the Addgene blog Read other posts on scientific sharing, scientific...

...GFP/EGFP) for a transcriptional reporter or as a general cytoplasmic label of cells, there's no problem.... of the FP. The FP is confined to a plane and generally only rotates in 360°, increasing the probability...can be monomerized with an A206K mutation [2]. Addgene has many of these constructs and they are designated...PMCID: PMC3324619. Additional Resources on the Addgene Blog Read Professor Snapp's Post on mOX FPs Find...in Your Experiments Additional Resources on Addgene.org Browse our Fluorescent Protein Resources Find...Plasmids that Glow (Luciferase Plasmids) Find Optogenetics Resources ...

...animation is becoming a TikTok series! Follow @addgene on TikTok for bite-sized portions of antibody concepts...an antibody?" or "what is an IgE isotype?"  @addgene Ready to learn about antibodies? Our new antibodies...molecularbiology #science #scicomm ♬ original sound - Addgene We'll be posting these animations regularly ... all of our videos will also be shared on our @addgene Instagram account, or you can watch the full animation...on our YouTube channel.  More resources on the Addgene blog Antibodies 101: Introduction to Antibodies...

...program are here. This was a great opportunity for Addgene to present our own data on plasmid deposits and...and distirbution for these fast moving fields.  Addgene is a global nonprofit plasmid repository. Over ...Over 2,000 labs have deposited plasmids to Addgene and we distribute over 130,000 plasmids in 2014. Thus,...combination of open-source distributors such as Addgene, as well as a number of online user forums such...Meeting Eric presented a poster with data comparing Addgene global deposit and distribution trends for Zinc...

...work is done in flow cytometers, and output is generated through measurement of events. An event is something... machine is plotted as a dot on an X,Y axis to generate a dot plot (Figure 1). Clustered events are called...                         Figure 1: While the plot generated often looks like the graph on the left, it might...intersection of X,Y. For flow plots, however, that is generally not true. Because of the variations in fluorescent...Of course, this is quite easy to do on a plot generated in Biorender! While a helpful visualization tool...10.1371/journal.pone.0137550 More resources on the Addgene blog Conventional vs. Flow Cytometry Plasmid-based...

...resources on the Addgene blog: Introduction to Immunofluorescence View all of Addgene's antibody posts  ...Resources on Addgene.org: Find plasmids encoding recombinant antibodies at Addgene  ...that interact with nucleic acids. Labs studying epigenetics routinely use the chromatin IP (ChIP) technique... found this blog helpful be sure to check out Addgene’s blog for other antibody topics.     References...

...open access journals.   Check Out Addgene Videos Over at the Addgene Youtube Channel   After watching ...what online tools people generally like to use to share ideas – with the general goal of seeing if and how...Communication 2.2 (2014): 2. Additional Resources on the Addgene Blog 6 Tools & Tips: Online Social Networking ...Sharing in Research Publications Resources at Addgene.org Check out our Protocol Pages for Video Protocols...

...Addgene Executive director, Joanne Kamens, recently participated in a Reddit AMA (short for “Ask Me Anything... sales-y in posts which falls in line with our general mission to share- as opposed to sell- science. ...serious career dilemmas. Some questions were about general advice on how to meet people, how to transition...content on many of the requested topics to the Addgene blog The blog has many other excellent posts on...and happy to help. Additional Resources on the Addgene Blog: 10 Ways to Share Your Science 6 Tools & ...How We're Sharing Science through Video with the Addgene Youtube Channel Check Out Vidoes of Gender & Science...

...pharmacology (many of which are represented in Addgene's Special Collections). Arguably, oncology has benefited...examining whether the cell lines had isoenzymes and genetic polymorphisms specific to their origins. This technique...out by the commercial repository. Repositories generally ensure cell line identity and purity making it...to the original as possible and so any results generated are reproducible and reliable. When you order ...Abcam Cell Culture Guidelines Resources on the Addgene Blog Learn All about Mammalian Vectors Read up...Using GFP for Protein Localization Resources on Addgene.org Find Plasmids for Mammalian Cells ...

...Science Communication Resources at Addgene.org Check out Careers at Addgene ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting...small tasks (like... preparing plasmids to send to Addgene). Following my advice from above, you should probably...appropriately. Back in my undergrad days, I had general goals for my experiments, but didn’t spend enough... in the comments! Additional Resources on the Addgene Blog Management for Scientists eBook Get Advice...

...engineered ascorbate peroxidase (APEX), used as a genetic tag for electron microscopy, could be used for ...(Che and Khavari, 2017). Find APEX plasmids at Addgene! Since the development of BioID and APEX, optimized...These newer tools are also currently available at Addgene.  BioID2: A smaller improved promiscuous biotin...utilized yeast display-based directed evolution to generate two mutants, the 35 kD TurboID, which has 15 mutations...et al. "Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy." Nature...PMCID: PMC5467174. Additional resources on the Addgene blog Plasmid 101 Protein Expression Plasmid 101...

...partnered with Addgene to make our collection of integrin antibodies available through Addgene’s repository...10.1038/s41467-022-35173-w More resources from Addgene Addgene's Antibody Guide Antibodies 101: Flow Cytometry...development, extended-open integrins enable tissue morphogenesis, organ development, and the shaping of the extracellular...on these cells. What’s next IPI is working with Addgene to accelerate biomedical research by distributing...

...remotely instead of from the lab. Nonetheless, Addgene’s annual Deck the Lab competition drew upon the ...Decoration Competition! #ScienceFun #ZoomParty @Addgene pic.twitter.com/drNBUdlQco — The Tarling-Vallim...CVRHutchinson #SciArt #STEAM #WinterWonderlab @Addgene Make your own here!: https://t.co/AnltWfKuQg pic.twitter.com...description of all the ornaments. #DeckTheLab @Addgene pic.twitter.com/GsAlD6sjM6 — Dr. Anne A Madden ...thank you to everyone who entered. Keep an eye on Addgene’s social media throughout the year for more fun ...

...ways of generating antibodies, for example through the use of less toxic adjuvants; 2) generating complex...and 4) generating mAbs against new cancer biomarkers. We believe that repositories, such as Addgene and ...The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years Data Freedom: The Expansion of...

...Selecting the Right Antibody Resources on Addgene.org Addgene's Antibody Guide  ...controls for biological experiments, starting with general controls and then moving on to controls for antibody-related...interpretation of results. A positive control is generally a sample or group that will have a desired response...address this, block positively charged groups with generally sticky proteins such as bovine serum albumin. ...Central PMCID: PMC2712964. More resources on the Addgene blog Antibodies 101: Validation Antibodies 101:...

... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR...is a single step gRNA multiplexing system that generates vectors containing high numbers of gRNAs in one...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...please let us know! Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

...into lentiviral vectors to generate a kit which is now available at Addgene. “We called it cloning by ...growth, survival, and differentiation.” https://www.addgene.org/kits/sabatini-wood-cancer-pathway-orfs/ Finding...PMCID: PMC4353587. Additional Resources on the Addgene Blog Learn How the Clon Tracer Library Has Been...Technologies from 2015 Additional Resources on Addgene.org Find Other Kits for Your Research Find Pooled...

...Internship with Addgene, I didn’t know a lot about scicomm, but had enjoyed writing a few Addgene guest blog...careers here Resources on Addgene.org Learn more about careers at Addgene ...Twitter @HansPacker. Additional Resources on the Addgene Blog Get tips on how to do your own informational...

...Resources on the Addgene Blog Plasmid Cloning by PCR Perform Site Directed Mutagenesis by PCR Catch up...that the exonuclease activity of the polymerase generates sticky ends in the PCR reactions. These sticky... currently an Outreach Data Curation Intern at Addgene. She is also a graduate student at the University...Background with our Plasmids 101 Posts Resources on Addgene.org Find Empty Vectors for Your Research Find Additional...

...Simplifying Plant Genetic Engineering Resources on Addgene.org Find Plant Vectors at Addgene Find Simple ...experiments outlined in ABRC’s kits vary by module. As a general guideline, experiments that include growing plants...-12 education.     Additional Resources on the Addgene Blog Learn how BioBuilder can bring Synthetic ...

... hear from Addgene’s former blog editor Tyler Ford on how initiative landed him at Addgene. What is initiative... Once in-person, peer-mentoring groups at Addgene have gone virtual!   2. Practice public speaking...almost every career talk I present, I’m asked what Addgene “looks for” when hiring a scientist. Of course,... comments below! Additional resources on the Addgene blog Read our science career blog posts Check ...

...lab used an alternative strategy to develop a genetically encoded antibody based probe that detects the...multi-color imaging. Find the HA frankenbody tool-kit at Addgene to get started with your experiments! What else..., T., Sato, Y., … Stasevich, T. J. (2019). A genetically encoded probe for imaging nascent and mature ...PMCID: PMC6610143. Additional resources on the Addgene blog Learn about visualizing protein turnover ...fluorescent protein blog posts Resources on Addgene.org Read our fluorescent protein guide Find plasmids...

...Resources on the Addgene Blog Perform Site Directed Mutagenesis by PCR Use REPLACR Mutatgenesis to Easily Generate... Generate Insertion and Deletions in a Plasmids Learn How to Verify Your Plasmid Resources on Addgene.org...