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We narrowed to 888 results for: URE

Showing: 701 - 750 of 888 results
  1. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...depending on the size of the conference). This made sure I didn’t zone out and kept me engaged throughout...If you’re presenting a poster at a conference, be sure to practice beforehand. Generally, two types of ...be on their website or the program booklet. Make sure you’re familiar with the policies before posting...
  2. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...available, you now face the challenge of trying to figure out what system might work best for your particular...vector reseals and other incorrect products. This feature biases the reaction in favor of the desired assembly...which one works best for you. Happy cloning (and be sure to deposit your finished constructs at Addgene!)...
  3. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...heterozygote into a homozygote and ensuring that all future offspring inherit the endonuclease gene. As we ...address many pressing global problems in health, agriculture, and conservation. But please be aware that there...biosafety officer should be familiar with appropriate measures. Barriers should be a component of all gene drive...
  4. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...and Fultineer analyzed the gnomAD dataset, which captures a broader swath of human genetic diversity, to...Project: a global resource to map genomic diversity. Nature 604, 437–446 (2022). https://doi.org/10.1038/s41586...weaponization of genetics research by extremists. Nature 610, 444-447 (2022). doi: 10.1038/d41586-022-03252...
  5. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...range of attention spans, skill levels, and STEM exposure. If you expect a lot of kids, you’re going to ... we can make this fun activity relate to STEM.” Figure 1 shows the activities we had for my school’s Math...Perhaps you have young children and you want to make sure their STEM education is supplemented. A 2009 study...
  6. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...vision in a canine model of childhood blindness. Nature Genetics 28, 92-95. PubMed PMID: 11326284. 4. Bennett...L., 2015. Gene Therapy returns to centre stage. Nature Vol 526, p.351. PubMed PMID: 26469046. 6. The Forever... Rev Virol. 2014 Nov;1(1):427-51. DOI: 10.1146/annurev-virology-031413-085355. PubMed PMID: 26958729.  ...
  7. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ... mean a world of mental relaxation. And, I’m not sure I should put this in print, but I actually let my... my daughter skip school a couple of times. Be sure to add time with your family, for yourself and for...just “do science” – we are scientists. We have to figure out a way to keep being ourselves and to be happy...
  8. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...and S. cerevisiae (Tuller et. al., 2010). Figure 1: An overview of peptide synthesis. A ribosome...as Hoogsten base pairs and wobble pairs.  Figure 2: (A) The codon wheel depicts the 64 codons that...PM, Li W-H (1987) The codon adaptation index-a measure of directional synonymous codon usage bias, and...
  9. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...The detectable signal from this reporter can be measured and quantified to determine the presence and quantity...lateral flow strip format. They published this in Nature Biotechnology. Like the SHERLOCK detection system...2020) CRISPR–Cas12-based detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038/s41587-020...
  10. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...behavioral tasks was an elevated plus maze that measures risk aversion/anxiety. The mouse can move freely..., learning, and memory. We’ll cover these in a  future blog post, so stay tuned!  References Alpár, ...rapid and long-lasting antidepressant effects." Nature communications 10.1 (2019): 223. PubMed PMID: 30644390...
  11. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    Published
    July 23, 2019, 12:46 p.m.
    ...transduction was not detected. They also used an ELISA to measure binding of PHP.B to a soluble version of LY6A. ...to the central and peripheral nervous systems." Nature neuroscience 20.8 (2017): 1172. PubMed PMID: 28671695...for widespread gene transfer to the adult brain." Nature biotechnology 34.2 (2016): 204. PubMed PMID: 26829320...
  12. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    Published
    April 2, 2019, 1:44 p.m.
    ... that lack cookie monster are sterile due to a failure of primary spermatocytes to enter meiosis. Testis...Dominguez, Roberto, and Kenneth C. Holmes. "Actin structure and function." (2011). PubMed PMID: 21314430. ...segment number and polarity in Drosophila." Nature287.5785 (1980): 795-801. PubMed PMID: 9390559. Additional...
  13. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ..., the envelope gene, and a polyA tail.   Figure 1: Lentiviral production uses three plasmids: (...in Medical Virology (Li et a., 2018).   Figure 2: To pseudotype with the SARS-CoV-2 spike protein..., Bieniasz PD, Jäger U, Porter CD, Friedman T, McClure MO, Weiss RA (1997) Sensitization of rhabdo-, lenti...
  14. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...Addgene. In vitro screening for the best gRNA Figure 1: Validation of double strand break (DSB) mediated...were fusing, you may have several mosaic mice. To figure out what alleles are present in these mice, you...favourite mammalian model organisms in the near future.  Many thanks to our guest blogger Samantha Young...
  15. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...than the other (Sandomenico et al., 2020). Figure 1: Comparison between the IgG antibody and scFv...of panning and scFv characterization.    Figure 2: Phage display starts with a pooled library of...Bates A, Power CA (2019) David vs. Goliath: The Structure, Function, and Clinical Prospects of Antibody ...
  16. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ... dsDNA breaks: following a cut, the error-prone nature of non-homologous end joining (NHEJ) often leads...database, which relies on CAGE-seq to directly capture the mRNA cap, provides the most accurate mapping...examined the ability to use sequence-based and other features to nominate gRNAs that are likely to be active...
  17. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ...pipeline processing (Nextflow), cloud infrastructure, and secure collaboration.  In that post, we talked...
  18. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...accompanied by new or refreshed figures made using BioRender.com. Figure 1: PRIME editing Please...
  19. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    Published
    Jan. 11, 2024, 2:15 p.m.
    ... significantly away from the functionality and features that previously made the platform a mainstay of...not delete @addgene on X. This will additionally ensure that the @addgene handle is always associated with...
  20. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ...the first day of CRISPR, Addgene gave to me: a miniature CRISPR-Cas system for mammalian use. On the second...Addgene gave to me: programmed genome editing by a miniature CRISPR-Cas12f nuclease. On the eighth day of CRISPR...
  21. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    Published
    Dec. 5, 2023, 2:15 p.m.
    ...sharing all the incredible guest posts and stories featured in our blog in 2023. Some of these were written...Course-Based Undergraduate Research Experiences (CUREs)Author: Jason PellettieriNew Acoustic Reporter Genes...
  22. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...interrogate many scientific questions. But these tiny creatures do tend to come with a rather astonishing ability...system for locating and containing escaped flies. Nature 4:1-22. https://doi.org/4.01.2022/hafd...
  23. 2021 Deck the Lab Winners!

    Type
    Blog Post
    Published
    Jan. 25, 2022, 2:15 p.m.
    ... by Xi Chen (@seleena78)   Honorable Mention: Culture Warrior The Moore lab, who was full of holiday ...honorable mention for their second submission.    Our Cultured Warrior (made by Nate using pipette boxes, wire...
  24. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    Published
    Oct. 8, 2019, 12:33 p.m.
    ..., you will be able to search for your favorite features, such as serotype, promoter, or tool type. Drilling...accepting data for materials distributed by Addgene.  Future directions As the AAV Data Hub grows, we hope to...
  25. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ...guide page. For more protocols and information, be sure to check out the Trono Lab website. If you have ... plasmids you’d like to deposit with Addgene, be sure to visit addgene.org/deposit. Who knows, maybe yours...
  26. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ...performing routine lab maintenance tasks. Hot tip: make sure your PI/facilities are okay with the decorations...@seleena78)   Here’s how to enter: Post your picture or video for either contest on Twitter or Instagram...
  27. Our New Antibody Mascot is....

    Type
    Blog Post
    Published
    Feb. 24, 2022, 2:15 p.m.
    ...  Fig. 1: Meet...well, frankly, we're not sure yet!   This as-yet–unnamed friend is a huge ...mascot, an antibody joining us on our scientific adventures.  You can submit your name suggestions here or...
  28. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    Published
    Nov. 16, 2020, 2:15 p.m.
    ...Fun stats and trivia begins! Tuesday: Depositor features (plasmid deposits and blog posts showcasing the...year in recent years. Despite COVID-19 and lab closures, we’re expecting to reach ~14,000 plasmids deposited...
  29. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    Published
    Jan. 14, 2021, 1:30 p.m.
    ...MicrobeAdventCalendar ornament set (plus more!) It features archaea, bacteria, fungi, viruses, and one microbe...Christmas tree! It is that time of the year again: miniature #Christmas tree goes up at home!This year with...
  30. Editor's Choice, October 2016

    Type
    Blog Post
    Published
    Nov. 4, 2016, 2:30 p.m.
    ...party (see all of our wonderful costumes in the pictures below, yours truly is dressed as Unikitty from...Changing Labor Laws Bring Increased Postdoc Wages,” Future of Research executive director, Gary McDowell, ...
  31. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    Published
    Sept. 1, 2016, 2:30 p.m.
    ... 26:10 : What's been your biggest experimental failure? 26:11 - 31: 32 : What's been your biggest experimental... Advice on Choosing a Good Mentor Learn How the Future of Research Looks to Improve the Research Process...
  32. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    Published
    Feb. 13, 2014, 1 p.m.
    ... are some good guidelines to follow in order to ensure that people can quickly and easily identify what...reading the name. For more information about plasmid features and tips you can use in the lab, check out more...
  33. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    Published
    Dec. 18, 2018, 1:17 p.m.
    ...compared to monomeric Cas9’s. Read the paper in Nature Communications Find the Cas9-Cas9 chimera plasmids... from this Addgene blog post Read the paper in Nature Methods Find the anti-CRISPR plasmids Tagging...
  34. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    Published
    Oct. 6, 2016, 2:30 p.m.
    ...here: https://www.youtube.com/watch?v=lQGAsgjjBDI&feature=youtu.be. 8:17 - 11:10 : The 3rd year of graduate... Advice on Choosing a Good Mentor Learn How the Future of Research Looks to Improve the Research Process...
  35. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    Published
    Nov. 13, 2015, 3:30 p.m.
    ...Seeing a real lab!” And several of our visitors made sure to tell us what we already knew about our Shipping... guests, as did how much work is put into making sure scientists all over the world know that we can help...
  36. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ..., along with links to the relevant scientific literature.  -In light of the current Ebola crisis, Science... anyone interested in EBOV’s past, present, and future, don’t miss Carl Zimmer’s coverage on the subject...
  37. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    Published
    Feb. 23, 2023, 2:15 p.m.
    ...present, but the real promise of Addgene lies in our future. As we near our 20th anniversary, we have many ...wealth of history to learn from, and a compelling future to plan in the changing landscape of science. We...
  38. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ...experiments; large amounts of virus are required to ensure that you get full library coverage in pooled library...and genome-wide libraries for CRISPR screening." Nature methods 11.8 (2014): 783-784. PubMed PMID: 25075903...
  39. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ... RNA binding domains depends on the secondary structure of RNA. As a result, targeting the RNA of interest...better understanding of the phenomenon soon.  Rest assured, as the PUF protein design tools continue to evolve...
  40. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    Published
    March 7, 2019, 2:39 p.m.
    ...started making regulations such as preventing manufacturers from dumping hazardous waste directly into the...levels. Disposables are an accepted part of lab culture, but with the right incentives (for example, by...
  41. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...sharing research materials — we're accelerating the future of science. Want to learn more about our latest...consensus platform for antibody characterization. Nature Protocols. https://doi.org/10.1038/s41596-024-01095...
  42. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ...King soundtrack and writing this post, it is my pleasure to announce that we once again reached new heights... remember the hazards of that chemical in your obscure growth medium, these apps can help make your lab...
  43. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ...Rather, they relied on published studies in the literature to curate the perfect library, obtained the appropriate...It could [be used to screen for] morphological features, migration, or survival in different contexts....
  44. Scientist Networking: What is an Informational Interview?

    Type
    Blog Post
    Published
    July 1, 2014, 3:42 p.m.
    ...focus wholly on the science I love. This immersive nature of academia often means that scientists-in-training... video call. If you do decide to use Skype, make sure to follow the rules of successful, remote conversations...
Showing: 701 - 750 of 888 results