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TypeBlog PostPublishedOct. 17, 2017, 12:55 p.m....interactive images of their proteins. Since the 1980’s, journal articles have been filled with impressive...
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Fluorescent Proteins 101: Introduction to FRET
TypeBlog PostPublishedJune 27, 2017, 1:03 p.m....in drug discovery. References 1. Baird, Geoffrey S., et al. "Circular permutation and receptor insertion... -
Progress Towards a PAM-Free CRISPR
TypeBlog PostPublishedMarch 7, 2024, 2:39 p.m.... Resources References Kwart, D., Paquet, D., Teo, S. et al. Precise and efficient scarless genome editing... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog PostPublishedSept. 26, 2019, 12:50 p.m....tropism of Nanoblades, different viral envelope protein(s) are expressed to pseudotype Nanoblades. The team ... -
Antibodies 101: Conventional vs Spectral Flow Cytometry
TypeBlog PostPublishedAug. 3, 2023, 1:15 p.m....overlapping markers. Compensation/reference control(s) Compensation or “reference” controls are samples ... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog PostPublishedDec. 14, 2021, 2:15 p.m....https://doi.org/10.1016/j.neuron.2013.08.032. Morita S, Noguchi H, Horii T et al. (2016) Targeted DNA demethylation... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog PostPublishedApril 6, 2021, 12:30 p.m....//doi.org/10.1089/hum.2019.277 Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD... -
Hot Plasmids - October 2020
TypeBlog PostPublishedOct. 13, 2020, 1:15 p.m....time that were not previously possible. Bensussen S, et al., iScience 2020. https://doi.org/10.1016/j.... -
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog PostPublishedMay 22, 2015, 4:58 p.m....science.aaa5945. PubMed. DiCarlo, J. E., Chavez, A., Dietz, S. L., Esvelt, K. M. & Church, G. M. RNA-guided gene... -
15 Hot Plasmids from 2017
TypeBlog PostPublishedJan. 9, 2018, 3:02 p.m....their useful reagents shared via Addgene. Narayan S, et al. Cell Reports. 2017. PubMed PMID: 28813671 ...the popular type II CRISPR systems, which include S. pyogenes (Spy) Cas9, been identified. In Rauch, et... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog PostPublishedApril 4, 2019, 12:53 p.m....in several bacteria associated with human disease (S. aureus, Klebsiella pneumoniae, and P. aeruginosa)... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m....DNA-scissors (blog). Blog website. 4. Hsu, Patrick D., Eric S. Lander, and Feng Zhang. "Development and applications... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog PostPublishedJune 22, 2017, 2:30 p.m....PubMed Central PMCID: PMC3074624. 5. Bindels, Daphne S., et al. "mScarlet: a bright monomeric red fluorescent... -
Pooled Library Amplifications
TypeBlog PostPublishedMay 30, 2024, 1:30 p.m....and how many cells to use for your transformation(s). The amount of competent cells you need total and... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog PostPublishedApril 18, 2024, 1:15 p.m....References and resources References Pyhtila, B., Kasowitz, S., Leeson, R., & Barrangou, R. (2023). The Expanding... -
Summer SciComm Series: Modes of Communication
TypeBlog PostPublishedJuly 1, 2022, 1:15 p.m....following four questions to figure out which method(s) work best for you, your audience, and your science... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog PostPublishedApril 23, 2014, 1:08 p.m....Many transfer vectors are SIN. Packaging plasmid(s) contain the minimal number of HIV genes required ... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog PostPublishedJuly 2, 2019, 12:51 p.m....lentivirus system pairs ubiquitous expression of sgRNA(s) and mCherry (vector 1) with neuron-specific expression... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog PostPublishedMay 30, 2019, 12:44 p.m....(2017): 1028-1043. PubMed PMID: 28841410. Qi, Lei S., et al. "Repurposing CRISPR as an RNA-guided platform... -
Plasmids 101: Shuttle Vectors
TypeBlog PostPublishedMay 20, 2025, 1:15 p.m.... can be used across species, for example, in both S. cerevisiae and E. coli. Auxotrophic selection is ...