We narrowed to 704 results for: ANG
-
TypeBlog PostPublishedApril 2, 2020, 6:17 p.m.... origin of replication (which controls the host range and copy number of the plasmid) and typically include...biologists to broadly utilize plasmids across a wide range of applications. Some common plasmid types include...Produce enzymes that will make specific, controlled changes to an organism’s genome (genome engineering) Produce...
-
An Addgene Summer Internship
TypeBlog PostPublishedAug. 8, 2024, 7:22 p.m....wanted to major in biochemistry. There, I had exchanged contact with Amy C., Addgene’s intern coordinator...into perspective just how much one’s environment changes them. Addgene gave me the chance to become a real...opened its arms to me from day one. Addgene has changed my life for the better, and I wouldn’t want it ... -
Embracing Serendipity: A Crucial Element in the PhD Journey
TypeBlog PostPublishedApril 25, 2024, 1:15 p.m.... of disciplines. Cast your net wider, explore tangential subjects, and let curiosity guide your intellectual...beyond your immediate field, fostering a diverse range of perspectives and sparking innovative thinking...embraces the unexpected and encourages exploration of tangents or seemingly unrelated ideas. It's beneficial ... -
Some Like it Hot: Thermostable GeoCas9
TypeBlog PostPublishedSept. 14, 2017, 12:40 p.m....). G. stearothermophilus grows at temperatures ranging from 30-74 °C, with an optimal growth temperature...or G) Spacer Length 20 nt 22 nt Temperature Range for Optimal Activity 35 - 45 °C 50 - 70 °C Genome...were incubated in reaction buffer at temperatures ranging from 25-80 °C. Activity is expressed as Kcleave... -
Choosing a Good Mentor for Scientists
TypeBlog PostPublishedJan. 16, 2014, 2:56 p.m....conflicts are a common reason for scientists to change labs – do as much as you can to ensure a good fit...possible. Attend a lab meeting. Spend some time hanging out with members of the lab. It is a warning sign...advisors and other related resources. Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog PostPublishedSept. 18, 2018, 1:33 p.m....LOV2 and Zdk1 to dissociate due to conformational changes, and as a consequence, EB1 protein function is ...manipulations on cells, and has potential use for a wide range of applications in cellular biology. Van Haren ...and Cas9 cleavage activity Article contributed by Angela Kaczmarczyk Listen to the base editing podcast... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog PostPublishedNov. 12, 2019, 2 p.m....ahead of time included contacting YNP staff to arrange permits to visit places not accessible to the public... around these hot springs make them extremely dangerous, we used telescoping poles to collect water samples...night was fun but also very tiring. It feels a bit strange to be using a micropipette and gloves at a campsite... -
Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation
TypeBlog PostPublishedNov. 8, 2018, 3:47 p.m....study it and see what happens when technological change happens really fast,” Thompson says. To do this... synthesis companies often work within a narrow range for GC content. Rare codons also are not good predictors... Jennifer Tsang... -
What Do I Do Now? Academic v. Non-Academic Career Decisions
TypeBlog PostPublishedMarch 9, 2017, 3:40 p.m....nice to have, not a need to have Must be ready to change projects frequently with little notice or choice...someone else who can do better, no grant writing Job changes can happen regularly (every 4-6 years is common... create interpersonal issues (one bad apple can change entire culture) Specific requirements for hiring... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog PostPublishedSept. 14, 2021, 1:15 p.m..../doi.org/10.2144/000112585 Ding T, Huang C, Liang Z, Ma X, Wang N, Huo Y-X (2020) Reversed paired-gRNA... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog PostPublishedJune 22, 2021, 1:15 p.m....Giandhari J, Pillay S, Wilkinson E, Naidoo Y, Karim F, Ganga Y, Khan K, Bernstein M, Balazs AB, Gosnell BI, Hanekom...03471-w Garcia-Beltran WF, Lam EC, Astudillo MG, Yang D, Miller TE, Feldman J, Hauser BM, Caradonna TM... Jennifer Tsang... -
Antibodies 101: Chimeric Antibodies
TypeBlog PostPublishedApril 25, 2023, 1:15 p.m....region may be changed. Chimeric antibodies can help A chimeric antibody is made by changing an antibody...will react with the same secondary. If you were to change one of the antibody’s isotypes, however, you could... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog PostPublishedJune 17, 2021, 1:17 p.m....activate transcription by causing conformation changes in the AraC dimer. In doing so, the dimer shifts...activation is. Figure 2: A bacteriograph of Michelangelo's "The Creation of Adam." This bacteriograph ... Jennifer Tsang... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog PostPublishedJune 9, 2016, 2:30 p.m....References 1. Gao, Feng, Xiao Z. Shen, Feng Jiang, Yongqiang Wu & Chunyu Han. “DNA-guided genome editing...expressed from a plasmid or in vitro transcribed, and changes in gRNA secondary structure can affect editing ... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog PostPublishedAug. 25, 2015, 2:30 p.m....devotion to seeing your ideas become something tangible. When you think of entrepreneurship in the 21st...company’s development Don’t be afraid to email strangers asking to meet. I have cold emailed hundreds of...including Harvard/MIT faculty, venture capital and angel investors, and business development heads at major... -
Mentoring for Scientists: I Have a Mentor, What Now?
TypeBlog PostPublishedMarch 6, 2014, 7:12 p.m....past the chatting stage you will rarely make any change in your skill sets or activate the helpful accountability...of working with a mentor.The responsibility of arranging meetings and setting the direction in a mentoring...for Choosing a Good Mentor", and more! Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring... -
Creating accessible biology activities in schools with BioBits
TypeBlog PostPublishedAug. 2, 2018, 12:56 p.m....This guest post was contributed by Ally Huang is a 4th year PhD student at MIT. While I had always enjoyed...make learning biology more fun – bit by bit. Ally Huang is a 4th year PhD student at MIT in Biological Engineering... can email her at [email protected]. References 1. Huang, Ally, et al. "BioBits™ Explorer: A modular synthetic... -
FPbase: A new community-editable fluorescent protein database
TypeBlog PostPublishedMay 16, 2018, 1 p.m.... tremendously complicated entities with a large range of characteristics (color, brightness, photostability...FPbase.org FPbase is a project written in Python & Django, using a popular database management system (PostgreSQL...users to see when a given page has unmoderated changes and to view previous versions. Currently, all ... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog PostPublishedNov. 27, 2018, 2 p.m.... these proteins will be tested. Investigating changes in neuronal activity after ischemic brain injury... Tsakanova to image cortical neurons and study changes in neural activity after ischemic brain injury.... diverse groups of scientists working in a wide range of research fields and geographical locations. In... -
Addgene Depositors Get More Citations
TypeBlog PostPublishedApril 20, 2017, 2:30 p.m....MIT Sloan School of Management. Professor Feng Zhang’s original 2013 gene editing paper on CRISPR/Cas ...In addition to publishing in Science, Professor Zhang deposited the associated plasmids with Addgene. ...School of Management. His research focuses on a range of topics including biotech and research tools, ...