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We narrowed to 889 results for: ANT;

Showing: 281 - 300 of 889 results

  1. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    Published
    Feb. 16, 2021, 2:15 p.m.
    ...employment, so it’s important to make sure a masters helps you get a job you want. But how do you know...I didn’t plan to get a master’s degree. I wanted to be a scientist and you need a PhD to be a scientist...prepared me to be exactly the type of scientist I wanted to be. But prior to graduate school, I wasn’t sure.... Don’t discount job postings looking for PhD applicants since many will also accept applications from...your work at conferences, help with manuscript and grant preparation, and lead your own research project.... lend itself more readily to a switch. 4. Do you want more autonomy?  Before my master’s, I worked in ... over. Everyday was the same and I was bored. I wanted more variety and more power to influence the direction...

  2. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...IgG) thus targeting MNase to antibody bound proteins (Figure 1). Importantly, since Protein A has weaker... culture, because it is easy to harvest large quantities of cells. However, when working on specific tissues...Washington. CUT&RUN doesn’t require fixation and significantly decreases background noise, reducing the read...dissections. With CUT&RUN, we have had success with significantly fewer wings, such that enough tissue for an ...beads. Nuclei are then incubated with a primary antibody against the protein of interest. The Protein A...is helpful to incubate with a rabbit secondary antibody when using a non-rabbit primary, prior to the ...improvements to the initial CUT&RUN protocol, the most significant being the usage of a strong detergent to permeabilize...

  3. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ...its advantages, most scientists still encounter several struggles with these techniques: unwanted mutations...chemically competent E. coli and transformants selected with the appropriate antibiotic. Although the precise mechanistic... isolation, insertion and amplification of a recombinant DNA without sequence alteration. Molecular cloning...overhangs, insert and vector purification, and, most importantly, excessive time requirements. FastCloning is ...ends. See an overview of the PCR process and the resultant products of the reaction in the figure from Chaokun... technique there is a chance of introducing an unwanted mutation. Tips for high efficiency cloning Now...Annabel HA Parret, and Anastassis Perrakis. "Recombinant cloning strategies for protein expression." Current...

  4. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...grateful to the grant officers at Fast Grants. We received a miraculous grant from Fast Grants, which was ... plasmids and to receive important coronavirus-related deposits. Our valiant lab and shipping staff agreed...collaborations as well as provides visibility for grant-driven research being done in all labs sharing materials...materials via Addgene. Perhaps if each of many grantors supporting research could provide Addgene with... for supporting Addgene by providing a generous grant. This support will enable us to continue all operations...

  5. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...produce recombinant proteins at high levels. In a case where clinical applications of recombinant proteins...most commonly used line expresses SV40 large T antigen and is thus dubbed HEK-293T. Given that the modern-day...HEKs. These systems built around HEKs offer an advantage to use the cell line and we will highlight some...production because they express the SV40 large T-antigen. This allows them to replicate viral plasmids with...viral particles are then filtered to remove any unwanted material (HEK cells, etc.) and are ready to use...also be adapted for serum-free growth, which significantly reduces the cost of production. Research applications...gene expression, growth rates, etc.). Thus, it’s important to maintain short culture periods (less than 20...

  6. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...said. “We know CREB is really important, but where is it really important and when? That is not known.”...injected, and they don’t always end up where you want them to go. Griesbeck is motivated by a particular... adds that this color change has a particular advantage for observing events taking place inside cells...from muscle cells. They identified a toadfish TnC variant which had a high-affinity for calcium binding and...Methods, the FRET changes of these Twitch sensor variants were optimized by testing various linker configurations...large-scale functional screen in bacteria. Sensor variants were then refined by a secondary screen in rat...“trick” to the development of additional color variants. This resulted in the construction of redCALWY...

  7. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ...contributed by guest blogger Joachim Goedart, an assistant professor at the Section of Molecular Cytology...fluorescent protein (GFP) from Aequorea victoria and its variants are genetically encoded fluorescent probes. One...Organized Smooth Endoplasmic Reticulum (OSER) assay (Costantini et al., 2012) measures homodimerization of FPs...the appearance of OSER whorls. This assay is quantitative and is currently the standard for assessing ...2016; Lambert, 2019). These characteristics are important, but have less priority than stickiness. In my...lead to over-expression artifacts. This is an important downside of the technology and should always be...linker length, the specific fluorescent protein variant, and where to add the fluorescent tag. These choices...

  8. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...Addgene’s Science Video Production Specialist, I’m constantly thinking about ways to improve our productions...going to be out of the office for longer than anticipated, we had to think of something else.   ...talking to their webcams.  Build: I sent the participants interview questions they could answer, and I...directing. Everyone’s schedules were busy, so it was important for me to let people record at their leisure. ...for our other videos. For starters, I hadn’t anticipated nearly every webcam recording only 1280x720 resolution... were still many restrictions in place, most importantly, masks.  Besides the muffled voices beneath masks...with them. I knew from my previous cycles that I wanted to be able to talk to people and direct them. For...

  9. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ... were corrupted by unwanted indels on the same allele, probably due to concomitant NHEJ repair. At the... cells, HDR is relatively rare and DSBs are predominantly repaired by NHEJ. While other groups have focused... locus, over 90% of the HDR edited alleles had unwanted insertions/deletions, therefore making those alleles...is accurately edited, the other likely contains unwanted indels. Following this observation, we tested ...introduction of an intended mutation while taking advantage of the improved efficiency provided by blocking...outcomes and in many cases still didn’t get what we wanted. The Indels were gone from most HDR reads that ...to direct the zygosity of the cells. This was important for us because the Alzheimer mutations we study...

  10. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    Published
    June 30, 2020, 1:15 p.m.
    ...format meant that the posters could be viewed publicly and stored in one place if the participants used ...involvement and how much time you want to spend with the conference. “If you want to have the same experience...first sent a pre-workshop questionnaire to the participants to learn about what they were looking to learn... content. In the middle of the workshop, the participants took part in a protocol drawing exercise. The...workshop also used a shared note-taking doc where participants could share their notes together. Figure...session you’re presenting is eye contact. “It’s important to keep your video on and look into the camera...there are many creative ways to adapt and take advantage of the new conference format. Additional resources...

  11. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    .... Sacks, Musicophilia includes case studies of relevant neurological atypicalities, including a patient...and fascinating, all without compromising on the relevant science."     - Randall Carter, Contracts Administrator...Microbes are everywhere and this book emphasizes the important roles microbes play in our ecosystem, examining...relationships between microbes and insects, mammals, and plants, among others. I particularly enjoyed the emphasis...series is the first 3 books of a larger epic of fantasy novels. Set in a world where 1000 years ago the... Norrell by Susanna Clarke "A quasi-historical/fantasy fiction. Two dueling magicians in the 1800s in ...launching this one. On a purely selfish level, I wanted to be challenged to read some books outside of ...

  12. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...the controversial medical issues. Participants gave significantly more favourable ratings to the abstracts... none of the participants had preconceived opinions about “adeolitis.” The participants in my study were...biochemistry, microbiology, epidemiology, and bioanthropology. Studying human health science made me happy...approached me for advice on her baby’s health but adamantly refused to get them vaccinated. A classmate told...told me that they never finish a course of antibiotics, choosing instead to save a few days’ worth of ... affirm or challenge their beliefs. I asked participants to read short abstracts comparing the mortality...texts were otherwise identical. Likewise, when participants read an abstract for a study with findings that...

  13. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ... your least abundant proteins, with the blue and infrared channels used for more abundant proteins or ...compatible with your microscope optics, you will want to compare the excitation and emission spectra of... microscope. Ideally, you would like to have substantial overlap between the excitation and emission filters...lecture at iBiology. Commonly used filter sets & relevant fluorescent proteins Commonly used filter sets... are mTagBFP2, EGFP or one of the improved GFP variants, mRuby2 or TagRFP-T, and an infrared fluorescent...In mammalian cells, one of the improved folding variants of EGFP like mEmerald or Clover is probably best...measurements. Here, EGFP outperforms the improved folding variants, presumably due to the lower growth temperature...

  14. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ...available. Table 1 compares some general advantages and disadvantages of each approach. Table 1: Properties...nighttime displays, these insects have contributed an important tool to the scientific community: luciferases,...levels and improved signal sensitivity. Another advantage: luciferase reporters aren’t susceptible to photobleaching...Japanese firefly (Luciola cruciata), and more. These variant proteins offer different kinetics, substrate requirements...luminescent substrate. This approach preserves the advantages of luminescent assays (low background, no photobleaching... that produce different colors or have other advantageous properties. This approach can improve live-cell...measurements of luminescence from live cells. Luciferase variants with incompatible substrates can also allow multiplexing...

  15. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...macromolecule density is equal to the buoyant force of the solution. Contaminants of lower densities are captured...Iodixanol Gradient Ultracentrifugation First, you’ll want to identify the reader’s goal. Why are they reading...What is the goal, who is my audience, and what do I want to be different for them when I’m done? What are...wait!  Done? Excellent! We asked our workshop participants to do the same thing, and even though only about...between layers are muddled due to the passage of supernatant through each layer. The right panel illustrates...before and after ultracentrifugation. The viral supernatant migrates through increasingly dense layers until..., and we’d love to hear yours as well.  More importantly, you can use these four steps for any type of...

  16. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...comparison of different enzymes. CasPEDIA is meant to be a constantly updating database which serves as a resource... Links to relevant resources The homepage displays a graphic describing CasIDs and relevant properties...field has exploded with dozens of Cas enzymes and variants available. Without a comprehensive resource, it... What are the features? CasPEDIA has collected relevant information on all the Cas enzymes you know and... know for viral packaging limitations. Maybe you want to read literature on the less common applications...experiment. Perhaps you are a structure junky who just wants to learn about Cas domains. CasPEDIA either directly...CRISPR–Cas systems: a burst of class 2 and derived variants. Nature Reviews Microbiology, 18(2), 67–83. https...

  17. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...function in terms of its regions. But equally important is the way the brain connects across these regions...is connected to itself across these regions is important for understanding how the brain functions. The...neurons that receive information from a region (anterograde tracer, Fig. 1B). Tracers are typically delivered...the soma (neuron cell body) toward the axon (anterograde tracer) or upstream from the axon terminals to...University of California, Berkeley engineered an AAV variant that efficiently infects neurons, directs high-...defined population of neurons. For example, say I wanted to access all the neurons that signaled from the... spinal cord (Wang, 2018, Metcalfe, 2022) and substantia nigra (Tervo, 2016). Discrepancies in transduction...

  18. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...cells you want to deliver to, and how you’ll be measuring your experimental output. You’ll also want to keep...serotypes can be transported retrogradely and/or anterogradely along the axon. Others, like AAV1 and AAV9, ...going to be working in several different systems or want to transduce a large number of different cells. ...et al., 2014; Nieuwenhuis et al., 2021). It is important to note that this expression pattern can be affected...however, increases with the size of the region you want to cover - and as your titer changes, so may your... is one that optimizes gene expression where you want it and minimizes expression where you don’t. The...Nonhuman Primates after Intramuscular Injection of Recombinant Adeno-Associated Virus. Journal of Virology, ...

  19. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    Published
    May 11, 2021, 1:15 p.m.
    ...the hM4Di, we used primary antibodies against mCherry, and secondary antibodies coupled to nanogold particles...Fluorescent proteins can also be identified using antibodies and immunohistochemistry techniques. Thus, these...transgenes of interest. Furthermore, fluorophore variants (for example green and red fluorescent proteins...Replacing the mCherry with the HA tag resulted in abundant expression of hM4Di in plasma membranes in both...results indicate that the tag protein may have an important influence on the expression of the transgene of...plasma membrane. Note that the use of the HA tag significantly improves the transport of hM4Di to the plasma...studies suggest that non-fluorescent tags may be advantageous over fluorescent proteins when the fluorescence...

  20. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...Taking advantage of Golden Gate cloning, the Haynes lab’s Protocols.io protocol describes an elegant system...also be applied to other fluorophore/antibody pairs. Because the anti-GFP nanobody cross-reacts with BFP...length can and should be varied as it can have important implications for the fusion protein effectivity...GFP on the surface and “receiver” cells express an anti-GFP nanobody on the cell surface. When the cells...both spatiotemporal protein degradation and quantification of this depletion using imaging-based measurements... the degradation of the AID-tagged protein. To quantify protein degradation the Ward lab used fluorescent...levels of dCas9 expression and contain different antibiotic resistance markers. New CRISPR pooled library...
Showing: 281 - 300 of 889 results