Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 1,092 results for: ANT;

Clear keywords
Showing: 1 - 20 of 1092 results

  1. Antibodies

    Type
    Collection
    ... Antibodies Addgene distributes ready-to-use recombinant antibodies to help facilitate...research. All Antibodies Ready to use antibodies? Browse Addgene’s collection of antibodies developed and... applications. Browse Antibodies Guide New to using antibodies? In our Antibody Guide, we define key terms... use your antibody. Antibody Blog Posts Recipient Instructions Addgene Help Center Antibody Protocols ...Overview of Addgene's ready-to-use monoclonal recombinant antibody collection. These affinity reagents are ... for Antibody Plasmids? Browse our collection of affinity reagent plasmids - including antibodies, nanobodies... terms, summarize antibody production and storage techniques, and explain common ...

  2. Antibody Guide

    Type
    Collection
    ... are called recombinant antibodies. Recombinant antibodies are created by cloning antibody genes from ...Science Guides Antibody Guide Antibody Guide View Addgene's Antibody Collection Jump To: Antibody Guide Production... produce antibodies, with the highest variability in antibodies produced. Cells - Antibodies can also ...amounts of a single antibody with little variation. Recombinant plasmids - Antibodies can be produced from...variable region and constant regions of the antibody to be independently manipulated. Antibodies produced this... in a buffer. Antibody Structure Figure 1: Structure of an antibody A standard antibody is made up of ...producing antibodies, can change the constant region, and therefore change the isotype of antibodies they ...

  3. All Antibodies

    Type
    Collection
    ... Antibodies All Antibodies All Antibodies Addgene distributes...distributes ready-to-use recombinant antibodies. These monoclonal antibodies undergo application-specific...each antibody, we provide important details including the protein and plasmid sequence, the antigen sequence...and secondary antibodies. To learn more about antibodies and their use, visit our Antibody Guide . Browse...new antibodies to add to the collection using our Antibody Suggestion Form . Browse All Antibodies ID ... use your antibody. Antibody Blog Posts Recipient Instructions Addgene Help Center Antibody Protocols ...collection of recombinant monoclonal antibodies. Antibodies...

  4. Antibody Production

    Type
    Collection
    ...target antigen is not endogenously-expressed (e.g., using an anti-GFP antibody), the target antigen is first...Addgene's antibody production methods and quality control processes for purified recombinant antibodies. Antibodies...Antibodies Antibody Production Antibody Production Many antibodies distributed by Addgene have been produced...the specific antibody webpage for further information. Production For recombinant antibodies (rAb) encoded... use your antibody. Antibody Blog Posts Recipient Instructions Addgene Help Center Antibody Protocols ...specific antibody in order to generate high quality preparations. Following production, all antibody preps...below. Information for a specific antibody can be found on the antibody's catalog page or by contacting help...

  5. Plant Plasmids and Resources

    Type
    Collection
    ... Plasmid Collections Plant Plasmids Plant Plasmids and Resources Cloning & SynBio ...designed for use in plants. These resources include tools for research using plant systems such as Arabidopsis... assembly of plant multigene constructs; contains yeast two-hybrid and bacterium-to-plant translocation...popular plasmids for gene editing in plants. Please refer to Addgene's Plant CRISPR Resources for a full selection...editing in plants. ID Kit Description Gene Editing Type PI 1000000076 GoldenBraid 2.0 Kit Plant synthetic...editing in dicotyledonous plants. CRISPR Johannes Stuttmann 1000000236 MoClo Plant Parts III: Transformation... our Plant CRISPR Resources for a selection of plasmids expressing different functional variants of Cas9...

  6. Antibody Plasmid Collection

    Type
    Collection
    ...Backbones for mouse recombinant antibodies A panel of recombinant monoclonal antibodies against zebrafish...ready-to-use recombinant antibodies made from plasmids in our collection! These monoclonal antibodies are produced...Learn More Cloning and Expressing Antibodies Antibody Plasmids Antibodies are large proteins made by the ...use synthetically produced recombinant antibodies, created by cloning antibody components into plasmids ...length recombinant monoclonal antibodies (R-mAbs) expressed in mammalian cells. Monoclonal antibodies are...about antibodies and their applications in our Antibody Guide ! Cloning and Expressing Antibodies The following...engineer the constant immunoglobulin domains to obtain recombinant hybridomas, which secrete antibodies as Fab...

  7. CRISPR Plasmids - Plants

    Type
    Collection
    ... CRISPR Plants CRISPR Plasmids: Plants Activate Base Edit Cut Interfere...have been designed for use in plants. CRISPR...following CRISPR plasmids have been designed for use in plants. To find emtpy gRNA plasmids, visit Addgene's Empty...Expression Vectors page and search the table for "Plant". Activate ID Plasmid Gene/Insert Promoter Selectable...

  8. Synthetic Biology - Plant

    Type
    Collection
    ... Plasmid Collections Synthetic Biology Plant Synthetic Biology: Plant SynBio Resources...collection of synthetic biology plasmids for use in plants. Plant Plasmids Search the table by keyword or sort...plasmids for use in plants. Plasmid...

  9. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...Introduction to Antibodies Plasmid-based Recombinant Antibodies Polyclonal Antibodies Resources on addgene.org...polyclonal antibodies are readily available from several sources, fewer sources of recombinant antibodies (rAbs.... Production Recombinant antibody production can take place in bacterial, yeast, plant, or mammalian ...At its core, harvesting recombinant antibodies consists of separating antibodies from media, cells, and...incubated with cell culture supernatant containing antibodies. The antibodies bind the immobilized ligands... Validation Recombinant antibodies should be validated to ensure they bind their antigens specifically...It’s important to note that some antibodies are better suited for certain applications. An antibody that...

  10. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...IgG2a 114557 Anti-TRIP8b (constant) [N269/73R] TRIP8b (constant) Rat Mouse IgG2a 114558 Anti-Synaptotagmin...IgG2a 177514 Anti-TRIP8b (constant) [N212/7R] TRIP8b (constant) Rat Mouse IgG2a 177515 Anti-RBM17/SPF45...IgG2a 177503 Anti-Pan-Gamma-protocadherin-Constant [N159/5R] Pan-Gamma-protocadherin-Constant Mouse IgG2a...applications. A substantial number of these have been cloned, expressed and validated in recombinant form. The...The recombinant monoclonal antibodies or R-mAbs have been engineered such that they are a distinct IgG... by the SV40 polyA tail (SV40 pA). Recombinant Monoclonal Antibody Plasmid Collection The following plasmids...Isotype 114473 Anti-TRIP8b (exon 4) [N212/3R] TRIP8b (exon 4) Rat Mouse IgG2a 114474 Anti-Clcn4 [N222/6R...

  11. Institute for Protein Innovation

    Type
    Collection
    ...Innovation Antibody Collection Axon Guidance Antibodies NEW Synaptic Cleft Antibodies Integrin Antibodies NEW...as well as antibody plasmids to create or express plasmid-based antibodies. Most antibodies in the collection...IPI’s Axon Guidance Antibody Collection comprises recombinant monoclonal antibodies with high specificity...Image credit: IPI Browse Contactin Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended...Browse Netrin, DCC, and IGDCC3 (PUNC) Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended...Applications PI Browse Neuropilin Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended...Applications PI Browse ROBO and Slit Antibodies ID Recombinant Antibody Description Isotype Reactivity Recommended...

  12. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...85048 pmScarlet_Giantin_C1 Golgi apparatus Giantin mScarlet Dorus Gadella 129630 mTurquoise2-Giantin Golgi...apparatus Giantin mTurquoise2 Dorus Gadella 98880 mNeonGreen-Giantin Golgi apparatus Giantin mNeonGreen...Vladislav Verkhusha 164464 H2B-mGreenLantern Chromatin H2B mGreenLantern Gregory Petsko 74257 pKanCMV-mClover3...164461 Mito-mGreenLantern Mitochondria 4x Mitochondrial targeting sequence (COX8) mGreenLantern Gregory Petsko...Plasma membrane (Plants) PIP2a mCherry Maria Harrison 61206 pCMU-PDESr Plasmodesmata (Plants) PDLP1 mCherry...Nefm EGFP Anthony Brown 32909 pEGFP-mNFM Intermediate filaments (neuronal) Nefm EGFP Anthony Brown 11908...William Bement 164459 LifeAct-mGreenLantern Actin filaments LifeAct mGreenLantern Gregory Petsko 212020 pcDNA3...

  13. CRISPR Guide

    Type
    Collection
    ...binding) SpCas9 VRER variant 3' NGCG SpCas9 EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN or NGNG xCas9...LbCpf1) 5' TTTV AsCpf1 RR variant 5' TYCV LbCpf1 RR variant 5' TYCV AsCpf1 RVR variant 5' TATV Campylobacter...PAM sequence often found in plants, expanding the targeting range for plant genome engineering. Read more...more about Cas9 variants . Anti-CRISPR Prolonged Cas9 activity can increase the risk of off-target cutting...like 3xFLAG-tag, PA, and biotin tags, or an anti-Cas9 antibody. The CAPTURE system, developed by the Jian...Species/Variant of Cas9 PAM Sequence* Streptococcus pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant 3' ...10.1089/crispr.2019.0030 PMID: 31742433 Durrant, M. G., Fanton, A., Tycko, J., Hinks, M., Chandrasekaran...

  14. Luciferase Plasmid Collection

    Type
    Collection
    ...Liqun Luo 51970 Nano-lantern/pcDNA3 Nano-lantern CMV Mammalian expression of Nano-lantern Takeharu Nagai 87121...luciferase . Each type of luciferase has advantages and disadvantages depending on the application, and different...our blog . Nano-Lanterns: Fluorescent proteins fused to Renilla luciferase ( Nano-lanterns ) or NanoLuc®...NanoLuc® ( Enhanced Nano-lanterns and Antares ) for in vivo imaging. NanoBRET : Constructs using NanoLuc® as...reporter. Unless otherwise noted, these plasmids are meant for expression in mammalian systems. ID Plasmid ...Creating ORF-Firefly luciferase gene fusions in plants. Renilla luciferase under the control of a CMV ...white screening can be used for screening of transformants in cloning process. Lynne Postovit 64034 pGL4Luc-RLuc...

  15. Immunology Research Plasmids and Resources

    Type
    Collection
    ...specific antigen, it finds and attacks pathogens or infected cells expressing that antigen. Antibodies produced...recognition by T cells of foreign antigens on the surface of antigen presenting cells (APC) including ...immunity through the secretion of antibodies, and also function as antigen presenting cells. B cells in secondary...immunoglobulin heavy constant alpha 2 (A2m marker) - IGHD immunoglobulin heavy constant delta FLJ00382, FLJ46727...IGHE immunoglobulin heavy constant epsilon IgE IGHG1 immunoglobulin heavy constant gamma 1 (G1m marker) -...

  16. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type
    Blog Post
    ...community.     Why recombinant antibodies? Animal-based methods for antibody production have several...Plückthun, 2015a, b). Recombinant antibodies are plasmid-based and offer numerous advantages, including more...polyclonal or monoclonal antibodies. You can learn more about recombinant antibodies in this blog post. Hello...ensure the quality of the antibodies using quantitative assays such as antibody specific activity for each...out our Antibodies 101 blog post Learn more about plasmid-based recombinant monoclonal antibodies Resources...received a grant from the National Institutes of Health that will address this problem.The grant, which is...Addgene to create a much needed open-access recombinant antibody/affinity reagent resource for the neuroscience...

  17. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...17798 pM-ErbB4CTF-PY1 mutant ERBB4 GAL4-BD ALS Marius Sudol 17799 pM-ErbB4CTF-PY3 mutant ERBB4 GAL4-BD ALS...delta-kinase-PY1 mutant ERBB4 GAL4-BD ALS Marius Sudol 17802 pM-ErbB4-delta-kinase-PY3 mutant ERBB4 GAL4-BD... Huntington's Nico Dantuma 23971 VCP(wt)-EGFP VCP His, GFP, HA CMV ALS Nico Dantuma 23972 VCP(R155H)-EGFP... HA CMV ALS Nico Dantuma 23973 VCP(A232E)-EGFP VCP His, GFP, HA CMV ALS Nico Dantuma 23974 VCP(DK0)-EGFP...pHA-deltaN335-Kin1A KIF5A HA CMV ALS Anthony Brown 31607 pKin1A KIF5A CMV ALS Anthony Brown 31717 pRK5M-ADAM10 ADAM10...Babu 128616 Anti-Kv1.1 K+ channel [K20/78R] KCNA1 CMV Episodic ataxia James Trimmer 128627 Anti-Kv4.3 K+ ...telangiectasia Charles Swanton 173580 Lenti-sgAtm#2/Cre ATM CMV Ataxia telangiectasia Charles Swanton 173759 pET-...

  18. Neurodegeneration Research Collection

    Type
    Collection
    ...related antibodies. Find antibodies to highly conserved proteins and other difficult-to-target antigens in ...Protein Innovation Antibody Collection . Explore the recombinant monoclonal antibodies and scFvs for neuroscience...One. 2011) Antibodies Find information on Addgene's collection of plasmid-based antibodies, including ... AAV Viral Preps iPSC Differentiation Factors Antibodies Fluorescent Proteins Other Resources Neurodegenerative...common disorders followed by a list of some of the relevant collections available at Addgene. Amyotrophic ...familial cases, usually inherited in an autosomal dominant manner, are associated with known genetic mutations...Huntington’s disease (HD) is a fatal autosomal dominant genetic disorder that causes the progressive degeneration...

  19. Neuroscience

    Type
    Collection
    ...learn more about antibodies and their use, visit our Antibody Guide . ID Recombinant Antibody Description ...Find recombinant antibodies targeting neuroscience-related proteins. Antibodies...Neuroscience Antibody Collection Browse or search the table below to find antibodies currently available from...neuroscience research. These ready-to-use recombinant monoclonal antibodies undergo application-specific validation...our current collection of Antibody Plasmids , fill out our Suggest an Antibody form, or e-mail [email protected]... Antibodies Neuroscience Neuroscience...Applications PI Do you have suggestions for other antibodies that should be added to this collection? Check...

  20. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...that you want to express a gene; however, we have included a function section for if you want to stably...vectors, are studying a different genetic element, or want to express shRNA. Choose by: Species-Specific Expression...Selectable Markers Species-Specific Expression If you want to drive expression of your favorite gene, you will...be functional in your host organism. Host Relevant Promoters Representative Empty Backbones Mammalian...Also see our Bacterial Expression collection page Plant CaMV35S, RPS5A, Ubiquitin pYPQ203 (pMDC32-Ubi1) ...resistance marker Also see more empty backbones on our Plant Plasmids and Resources collection page Yeast GAL4... to easily identify your protein using an antibody against that epitope. This could allow you...
Showing: 1 - 20 of 1092 results